Experiment: McCas_H2_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02530 and MMP_RS02535 overlap by 4 nucleotides MMP_RS02535 and MMP_RS02540 are separated by 148 nucleotides MMP_RS02540 and MMP_RS02545 are separated by 586 nucleotides
MMP_RS02530: MMP_RS02530 - type II toxin-antitoxin system mRNA interferase toxin RelE/StbE family, at 473,717 to 474,019
_RS02530
MMP_RS02535: MMP_RS02535 - DUF2683 family protein, at 474,016 to 474,264
_RS02535
MMP_RS02540: MMP_RS02540 - DUF2283 domain-containing protein, at 474,413 to 474,790
_RS02540
MMP_RS02545: MMP_RS02545 - hypothetical protein, at 475,377 to 475,787
_RS02545
Position (kb)
474
475 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 473.445 kb on + strand at 473.450 kb on - strand at 473.460 kb on - strand at 473.650 kb on + strand at 473.754 kb on - strand, within MMP_RS02530 at 473.755 kb on + strand, within MMP_RS02530 at 473.776 kb on + strand, within MMP_RS02530 at 473.787 kb on - strand, within MMP_RS02530 at 473.787 kb on - strand, within MMP_RS02530 at 473.787 kb on - strand, within MMP_RS02530 at 473.788 kb on + strand, within MMP_RS02530 at 473.789 kb on - strand, within MMP_RS02530 at 473.838 kb on - strand, within MMP_RS02530 at 473.948 kb on + strand, within MMP_RS02530 at 473.989 kb on - strand at 474.169 kb on - strand at 474.284 kb on + strand at 474.306 kb on - strand at 474.319 kb on - strand at 474.323 kb on - strand at 474.324 kb on + strand at 474.367 kb on + strand at 474.368 kb on - strand at 474.369 kb on + strand at 474.374 kb on - strand at 474.375 kb on + strand at 474.657 kb on + strand, within MMP_RS02540 at 474.714 kb on - strand, within MMP_RS02540 at 474.742 kb on + strand, within MMP_RS02540 at 474.742 kb on + strand, within MMP_RS02540 at 474.746 kb on - strand, within MMP_RS02540 at 474.746 kb on - strand, within MMP_RS02540 at 474.746 kb on - strand, within MMP_RS02540 at 474.747 kb on + strand, within MMP_RS02540 at 474.766 kb on + strand at 474.814 kb on - strand at 474.814 kb on - strand at 474.839 kb on - strand at 474.840 kb on + strand at 474.841 kb on - strand at 474.841 kb on - strand at 474.846 kb on - strand at 474.847 kb on + strand at 474.847 kb on + strand at 474.852 kb on + strand at 474.880 kb on + strand at 474.883 kb on - strand at 474.900 kb on + strand at 475.037 kb on - strand at 475.271 kb on - strand at 475.350 kb on - strand at 475.413 kb on + strand at 475.414 kb on - strand at 475.415 kb on + strand at 475.459 kb on + strand, within MMP_RS02545 at 475.480 kb on + strand, within MMP_RS02545 at 475.504 kb on + strand, within MMP_RS02545 at 475.548 kb on - strand, within MMP_RS02545 at 475.605 kb on + strand, within MMP_RS02545 at 475.710 kb on - strand, within MMP_RS02545 at 475.724 kb on + strand, within MMP_RS02545
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_H2_complete Antimycin A 0.01 mM remove 473,445 + -0.6 473,450 - +4.3 473,460 - -0.8 473,650 + -0.5 473,754 - MMP_RS02530 0.12 +0.4 473,755 + MMP_RS02530 0.13 -1.2 473,776 + MMP_RS02530 0.19 +0.2 473,787 - MMP_RS02530 0.23 -1.3 473,787 - MMP_RS02530 0.23 -0.4 473,787 - MMP_RS02530 0.23 +1.8 473,788 + MMP_RS02530 0.23 +0.1 473,789 - MMP_RS02530 0.24 -1.7 473,838 - MMP_RS02530 0.40 -0.6 473,948 + MMP_RS02530 0.76 +0.9 473,989 - +1.0 474,169 - -1.7 474,284 + -2.6 474,306 - -2.2 474,319 - -1.9 474,323 - -0.6 474,324 + +0.4 474,367 + +1.2 474,368 - -0.8 474,369 + -0.0 474,374 - -0.7 474,375 + -0.2 474,657 + MMP_RS02540 0.65 +0.3 474,714 - MMP_RS02540 0.80 -1.1 474,742 + MMP_RS02540 0.87 +0.1 474,742 + MMP_RS02540 0.87 -0.3 474,746 - MMP_RS02540 0.88 -1.2 474,746 - MMP_RS02540 0.88 -2.0 474,746 - MMP_RS02540 0.88 -1.5 474,747 + MMP_RS02540 0.88 +0.6 474,766 + -0.7 474,814 - +0.6 474,814 - +2.5 474,839 - -0.5 474,840 + +0.7 474,841 - +0.7 474,841 - -0.2 474,846 - -1.0 474,847 + -0.0 474,847 + +0.3 474,852 + +0.4 474,880 + +1.6 474,883 - +3.0 474,900 + +0.5 475,037 - +1.3 475,271 - +0.8 475,350 - -1.1 475,413 + +0.2 475,414 - -0.1 475,415 + -1.2 475,459 + MMP_RS02545 0.20 -0.7 475,480 + MMP_RS02545 0.25 -0.8 475,504 + MMP_RS02545 0.31 +1.2 475,548 - MMP_RS02545 0.42 -2.5 475,605 + MMP_RS02545 0.55 -1.0 475,710 - MMP_RS02545 0.81 -1.6 475,724 + MMP_RS02545 0.84 -1.1
Or see this region's nucleotide sequence