Experiment: McCas_H2_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02445 and MMP_RS02450 are separated by 273 nucleotides MMP_RS02450 and MMP_RS02455 are separated by 12 nucleotides
MMP_RS02445: MMP_RS02445 - putative DNA binding domain-containing protein, at 456,701 to 458,044
_RS02445
MMP_RS02450: MMP_RS02450 - hypothetical protein, at 458,318 to 458,512
_RS02450
MMP_RS02455: MMP_RS02455 - hypothetical protein, at 458,525 to 459,142
_RS02455
Position (kb) 456
457
458
459 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 455.803 kb on - strand at 455.804 kb on + strand at 455.813 kb on - strand at 455.813 kb on - strand at 455.814 kb on + strand at 455.817 kb on - strand at 455.817 kb on - strand at 455.817 kb on - strand at 455.818 kb on + strand at 455.822 kb on - strand at 455.822 kb on - strand at 455.822 kb on - strand at 455.823 kb on + strand at 455.823 kb on + strand at 455.825 kb on + strand at 455.825 kb on + strand at 455.825 kb on + strand at 455.827 kb on - strand at 455.835 kb on - strand at 455.835 kb on - strand at 455.836 kb on + strand at 455.836 kb on + strand at 455.836 kb on + strand at 455.836 kb on + strand at 455.840 kb on + strand at 455.856 kb on + strand at 455.859 kb on - strand at 455.860 kb on + strand at 455.860 kb on + strand at 455.864 kb on - strand at 455.865 kb on + strand at 455.901 kb on - strand at 456.003 kb on - strand at 456.009 kb on - strand at 456.021 kb on + strand at 456.027 kb on - strand at 456.028 kb on + strand at 456.064 kb on - strand at 456.065 kb on + strand at 456.065 kb on + strand at 456.119 kb on - strand at 456.119 kb on - strand at 456.128 kb on - strand at 456.128 kb on - strand at 456.128 kb on - strand at 456.173 kb on - strand at 456.202 kb on + strand at 456.239 kb on - strand at 456.239 kb on - strand at 456.240 kb on + strand at 456.240 kb on + strand at 456.240 kb on + strand at 456.245 kb on + strand at 456.274 kb on + strand at 456.274 kb on + strand at 456.328 kb on - strand at 456.331 kb on + strand at 456.475 kb on - strand at 456.493 kb on - strand at 456.494 kb on + strand at 456.550 kb on - strand at 456.551 kb on + strand at 456.554 kb on - strand at 456.554 kb on - strand at 456.557 kb on + strand at 456.557 kb on + strand at 456.559 kb on + strand at 456.559 kb on + strand at 456.559 kb on + strand at 456.559 kb on + strand at 456.559 kb on + strand at 456.625 kb on + strand at 456.655 kb on + strand at 456.666 kb on - strand at 456.673 kb on - strand at 456.674 kb on + strand at 456.678 kb on - strand at 456.678 kb on - strand at 456.678 kb on - strand at 456.680 kb on - strand at 456.681 kb on + strand at 456.736 kb on + strand at 456.738 kb on - strand at 456.739 kb on + strand at 456.755 kb on - strand at 456.758 kb on + strand at 456.805 kb on + strand at 456.975 kb on - strand, within MMP_RS02445 at 457.028 kb on - strand, within MMP_RS02445 at 457.028 kb on - strand, within MMP_RS02445 at 457.028 kb on - strand, within MMP_RS02445 at 457.029 kb on + strand, within MMP_RS02445 at 457.229 kb on + strand, within MMP_RS02445 at 457.239 kb on - strand, within MMP_RS02445 at 457.240 kb on + strand, within MMP_RS02445 at 457.240 kb on + strand, within MMP_RS02445 at 457.243 kb on + strand, within MMP_RS02445 at 457.289 kb on + strand, within MMP_RS02445 at 457.289 kb on + strand, within MMP_RS02445 at 457.318 kb on - strand, within MMP_RS02445 at 457.319 kb on + strand, within MMP_RS02445 at 457.334 kb on - strand, within MMP_RS02445 at 457.450 kb on + strand, within MMP_RS02445 at 457.519 kb on - strand, within MMP_RS02445 at 457.560 kb on - strand, within MMP_RS02445 at 457.561 kb on + strand, within MMP_RS02445 at 457.564 kb on - strand, within MMP_RS02445 at 457.564 kb on - strand, within MMP_RS02445 at 457.564 kb on - strand, within MMP_RS02445 at 457.564 kb on - strand, within MMP_RS02445 at 457.564 kb on - strand, within MMP_RS02445 at 457.565 kb on + strand, within MMP_RS02445 at 457.565 kb on + strand, within MMP_RS02445 at 457.566 kb on - strand, within MMP_RS02445 at 457.566 kb on - strand, within MMP_RS02445 at 457.566 kb on - strand, within MMP_RS02445 at 457.567 kb on + strand, within MMP_RS02445 at 457.567 kb on + strand, within MMP_RS02445 at 457.605 kb on - strand, within MMP_RS02445 at 457.606 kb on + strand, within MMP_RS02445 at 457.611 kb on - strand, within MMP_RS02445 at 457.611 kb on - strand, within MMP_RS02445 at 457.622 kb on - strand, within MMP_RS02445 at 457.675 kb on + strand, within MMP_RS02445 at 457.716 kb on - strand, within MMP_RS02445 at 457.879 kb on + strand, within MMP_RS02445 at 457.919 kb on + strand at 457.932 kb on - strand at 457.942 kb on - strand at 457.943 kb on + strand at 457.996 kb on + strand at 457.996 kb on + strand at 458.048 kb on + strand at 458.048 kb on + strand at 458.048 kb on + strand at 458.054 kb on + strand at 458.054 kb on + strand at 458.064 kb on - strand at 458.087 kb on + strand at 458.098 kb on + strand at 458.136 kb on + strand at 458.136 kb on + strand at 458.214 kb on - strand at 458.231 kb on + strand at 458.250 kb on + strand at 458.308 kb on - strand at 458.347 kb on + strand, within MMP_RS02450 at 458.361 kb on - strand, within MMP_RS02450 at 458.371 kb on - strand, within MMP_RS02450 at 458.392 kb on - strand, within MMP_RS02450 at 458.392 kb on - strand, within MMP_RS02450 at 458.398 kb on + strand, within MMP_RS02450 at 458.398 kb on + strand, within MMP_RS02450 at 458.403 kb on + strand, within MMP_RS02450 at 458.468 kb on + strand, within MMP_RS02450 at 458.532 kb on + strand at 458.628 kb on - strand, within MMP_RS02455 at 458.629 kb on + strand, within MMP_RS02455 at 458.630 kb on - strand, within MMP_RS02455 at 458.760 kb on + strand, within MMP_RS02455 at 458.783 kb on - strand, within MMP_RS02455 at 458.787 kb on - strand, within MMP_RS02455 at 458.787 kb on - strand, within MMP_RS02455 at 458.960 kb on + strand, within MMP_RS02455 at 458.963 kb on - strand, within MMP_RS02455 at 458.974 kb on - strand, within MMP_RS02455 at 458.974 kb on - strand, within MMP_RS02455 at 458.996 kb on + strand, within MMP_RS02455
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_H2_complete Antimycin A 0.01 mM remove 455,803 - +0.9 455,804 + +1.0 455,813 - +0.4 455,813 - -1.7 455,814 + -1.5 455,817 - +1.3 455,817 - -0.9 455,817 - -1.4 455,818 + +1.1 455,822 - +1.3 455,822 - +1.0 455,822 - +1.2 455,823 + +1.0 455,823 + -0.1 455,825 + -0.7 455,825 + +1.0 455,825 + +0.1 455,827 - -2.2 455,835 - +0.0 455,835 - -0.2 455,836 + +0.2 455,836 + -0.5 455,836 + +1.4 455,836 + -1.5 455,840 + -0.1 455,856 + +0.8 455,859 - +0.7 455,860 + +0.9 455,860 + +0.6 455,864 - +1.4 455,865 + +0.8 455,901 - -0.5 456,003 - -1.2 456,009 - -1.1 456,021 + +1.8 456,027 - -0.6 456,028 + +0.5 456,064 - -0.9 456,065 + +0.0 456,065 + +0.1 456,119 - +1.4 456,119 - -1.0 456,128 - -0.2 456,128 - -2.4 456,128 - +0.2 456,173 - +0.8 456,202 + +1.1 456,239 - +0.8 456,239 - +0.0 456,240 + +1.5 456,240 + +0.4 456,240 + +0.9 456,245 + +1.9 456,274 + +0.1 456,274 + +0.8 456,328 - -0.8 456,331 + +0.9 456,475 - +0.3 456,493 - +2.8 456,494 + -1.2 456,550 - -0.2 456,551 + -0.6 456,554 - -1.3 456,554 - +1.9 456,557 + +2.0 456,557 + +0.2 456,559 + +1.6 456,559 + +2.0 456,559 + -0.9 456,559 + +1.1 456,559 + -0.6 456,625 + +0.5 456,655 + -0.4 456,666 - +0.4 456,673 - +0.1 456,674 + -0.4 456,678 - -0.0 456,678 - +0.4 456,678 - -0.6 456,680 - -0.3 456,681 + +4.2 456,736 + +1.0 456,738 - +0.3 456,739 + -1.1 456,755 - +1.0 456,758 + +0.2 456,805 + -0.8 456,975 - MMP_RS02445 0.20 -0.8 457,028 - MMP_RS02445 0.24 +0.3 457,028 - MMP_RS02445 0.24 -0.2 457,028 - MMP_RS02445 0.24 +0.1 457,029 + MMP_RS02445 0.24 +1.2 457,229 + MMP_RS02445 0.39 +0.2 457,239 - MMP_RS02445 0.40 +0.5 457,240 + MMP_RS02445 0.40 +0.8 457,240 + MMP_RS02445 0.40 -0.3 457,243 + MMP_RS02445 0.40 +1.0 457,289 + MMP_RS02445 0.44 +3.5 457,289 + MMP_RS02445 0.44 -0.1 457,318 - MMP_RS02445 0.46 +0.7 457,319 + MMP_RS02445 0.46 -0.2 457,334 - MMP_RS02445 0.47 -0.4 457,450 + MMP_RS02445 0.56 +1.2 457,519 - MMP_RS02445 0.61 +2.2 457,560 - MMP_RS02445 0.64 -0.6 457,561 + MMP_RS02445 0.64 +1.4 457,564 - MMP_RS02445 0.64 +0.5 457,564 - MMP_RS02445 0.64 +0.7 457,564 - MMP_RS02445 0.64 +0.3 457,564 - MMP_RS02445 0.64 -0.6 457,564 - MMP_RS02445 0.64 -0.9 457,565 + MMP_RS02445 0.64 +1.4 457,565 + MMP_RS02445 0.64 +1.2 457,566 - MMP_RS02445 0.64 +0.4 457,566 - MMP_RS02445 0.64 +0.2 457,566 - MMP_RS02445 0.64 +1.0 457,567 + MMP_RS02445 0.64 +1.1 457,567 + MMP_RS02445 0.64 +2.0 457,605 - MMP_RS02445 0.67 +0.6 457,606 + MMP_RS02445 0.67 -0.4 457,611 - MMP_RS02445 0.68 +1.0 457,611 - MMP_RS02445 0.68 -0.1 457,622 - MMP_RS02445 0.69 +0.0 457,675 + MMP_RS02445 0.72 +0.4 457,716 - MMP_RS02445 0.76 -1.6 457,879 + MMP_RS02445 0.88 +1.0 457,919 + -1.2 457,932 - -4.2 457,942 - -0.7 457,943 + -1.0 457,996 + -0.3 457,996 + -1.2 458,048 + +0.5 458,048 + +0.4 458,048 + -2.1 458,054 + +2.0 458,054 + +0.9 458,064 - +1.0 458,087 + +0.2 458,098 + +0.4 458,136 + -0.5 458,136 + +0.4 458,214 - -0.3 458,231 + +0.9 458,250 + +0.3 458,308 - +0.9 458,347 + MMP_RS02450 0.15 +0.1 458,361 - MMP_RS02450 0.22 +0.8 458,371 - MMP_RS02450 0.27 +0.2 458,392 - MMP_RS02450 0.38 +1.0 458,392 - MMP_RS02450 0.38 -0.9 458,398 + MMP_RS02450 0.41 -0.3 458,398 + MMP_RS02450 0.41 -2.4 458,403 + MMP_RS02450 0.44 +0.5 458,468 + MMP_RS02450 0.77 -1.0 458,532 + -1.8 458,628 - MMP_RS02455 0.17 +0.2 458,629 + MMP_RS02455 0.17 -1.5 458,630 - MMP_RS02455 0.17 -1.4 458,760 + MMP_RS02455 0.38 +1.3 458,783 - MMP_RS02455 0.42 -0.3 458,787 - MMP_RS02455 0.42 +2.2 458,787 - MMP_RS02455 0.42 +0.7 458,960 + MMP_RS02455 0.70 +0.8 458,963 - MMP_RS02455 0.71 -1.0 458,974 - MMP_RS02455 0.73 +0.8 458,974 - MMP_RS02455 0.73 +0.6 458,996 + MMP_RS02455 0.76 +0.8
Or see this region's nucleotide sequence