Experiment: McCas_H2_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00710 and MMP_RS00715 are separated by 180 nucleotides MMP_RS00715 and MMP_RS00720 are separated by 27 nucleotides MMP_RS00720 and MMP_RS00725 are separated by 40 nucleotides
MMP_RS00710: MMP_RS00710 - hypothetical protein, at 127,981 to 128,889
_RS00710
MMP_RS00715: MMP_RS00715 - formate-dependent phosphoribosylglycinamide formyltransferase, at 129,070 to 130,236
_RS00715
MMP_RS00720: MMP_RS00720 - metal-dependent hydrolase, at 130,264 to 130,938
_RS00720
MMP_RS00725: MMP_RS00725 - 5 10-methenyltetrahydromethanopterin hydrogenase cofactor biosynthesis protein HmdC, at 130,979 to 132,511
_RS00725
Position (kb) 129
130
131 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 128.205 kb on - strand, within MMP_RS00710 at 128.546 kb on + strand, within MMP_RS00710 at 128.546 kb on + strand, within MMP_RS00710 at 128.777 kb on + strand, within MMP_RS00710 at 128.800 kb on - strand at 128.800 kb on - strand at 128.829 kb on - strand at 128.858 kb on + strand at 128.858 kb on + strand at 128.859 kb on - strand at 128.859 kb on - strand at 128.967 kb on - strand at 128.989 kb on - strand at 128.990 kb on + strand at 128.995 kb on + strand at 128.996 kb on - strand at 128.996 kb on - strand at 128.996 kb on - strand at 128.999 kb on - strand at 129.001 kb on - strand at 129.028 kb on - strand at 129.225 kb on - strand at 129.638 kb on - strand at 129.875 kb on - strand at 130.010 kb on - strand at 130.115 kb on - strand at 130.146 kb on - strand at 130.284 kb on + strand at 130.292 kb on + strand at 130.477 kb on + strand, within MMP_RS00720 at 130.534 kb on + strand, within MMP_RS00720 at 130.650 kb on - strand, within MMP_RS00720 at 130.683 kb on - strand, within MMP_RS00720 at 130.684 kb on + strand, within MMP_RS00720 at 130.685 kb on - strand, within MMP_RS00720 at 130.748 kb on + strand, within MMP_RS00720 at 130.751 kb on - strand, within MMP_RS00720 at 130.752 kb on + strand, within MMP_RS00720 at 130.773 kb on - strand, within MMP_RS00720 at 130.790 kb on - strand, within MMP_RS00720 at 130.791 kb on + strand, within MMP_RS00720 at 130.807 kb on - strand, within MMP_RS00720 at 130.808 kb on + strand, within MMP_RS00720 at 130.819 kb on - strand, within MMP_RS00720 at 130.831 kb on - strand, within MMP_RS00720 at 130.887 kb on - strand at 130.907 kb on + strand at 130.918 kb on + strand at 130.986 kb on + strand at 131.041 kb on - strand at 131.092 kb on - strand at 131.101 kb on + strand at 131.101 kb on + strand at 131.101 kb on + strand at 131.123 kb on - strand at 131.173 kb on + strand, within MMP_RS00725 at 131.173 kb on + strand, within MMP_RS00725 at 131.174 kb on - strand, within MMP_RS00725 at 131.174 kb on - strand, within MMP_RS00725 at 131.175 kb on + strand, within MMP_RS00725
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_H2_complete Antimycin A 0.01 mM remove 128,205 - MMP_RS00710 0.25 +0.9 128,546 + MMP_RS00710 0.62 -1.8 128,546 + MMP_RS00710 0.62 +0.7 128,777 + MMP_RS00710 0.88 -0.2 128,800 - +0.4 128,800 - -0.3 128,829 - +1.1 128,858 + -1.1 128,858 + +0.5 128,859 - -1.3 128,859 - +3.4 128,967 - +1.0 128,989 - +0.2 128,990 + -2.2 128,995 + +1.6 128,996 - +0.1 128,996 - +1.0 128,996 - +1.0 128,999 - -2.0 129,001 - -0.6 129,028 - -1.0 129,225 - -1.9 129,638 - +0.5 129,875 - +0.6 130,010 - -0.4 130,115 - -2.6 130,146 - -2.1 130,284 + +0.4 130,292 + -1.7 130,477 + MMP_RS00720 0.32 -0.1 130,534 + MMP_RS00720 0.40 -0.6 130,650 - MMP_RS00720 0.57 -0.3 130,683 - MMP_RS00720 0.62 -1.9 130,684 + MMP_RS00720 0.62 -0.4 130,685 - MMP_RS00720 0.62 -0.5 130,748 + MMP_RS00720 0.72 -0.4 130,751 - MMP_RS00720 0.72 -0.5 130,752 + MMP_RS00720 0.72 -1.1 130,773 - MMP_RS00720 0.75 -2.1 130,790 - MMP_RS00720 0.78 -1.5 130,791 + MMP_RS00720 0.78 +0.1 130,807 - MMP_RS00720 0.80 -0.1 130,808 + MMP_RS00720 0.81 -0.5 130,819 - MMP_RS00720 0.82 -1.7 130,831 - MMP_RS00720 0.84 -0.9 130,887 - +0.5 130,907 + -1.0 130,918 + +0.8 130,986 + -0.8 131,041 - -0.6 131,092 - -1.0 131,101 + -1.4 131,101 + -1.1 131,101 + -2.1 131,123 - -1.4 131,173 + MMP_RS00725 0.13 -0.7 131,173 + MMP_RS00725 0.13 -0.5 131,174 - MMP_RS00725 0.13 -0.6 131,174 - MMP_RS00725 0.13 -0.4 131,175 + MMP_RS00725 0.13 -2.6
Or see this region's nucleotide sequence