Experiment: McCas_H2_complete Irgasan 0.025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS04515 and MMP_RS04520 are separated by 443 nucleotides MMP_RS04520 and MMP_RS04525 are separated by 31 nucleotides MMP_RS04525 and MMP_RS04530 are separated by 41 nucleotides
MMP_RS04515: MMP_RS04515 - betaine/proline/choline family ABC transporter ATP-binding protein, at 859,546 to 860,715
_RS04515
MMP_RS04520: MMP_RS04520 - nucleoside deaminase, at 861,159 to 861,596
_RS04520
MMP_RS04525: MMP_RS04525 - D-2-hydroxyacid dehydrogenase, at 861,628 to 862,584
_RS04525
MMP_RS04530: MMP_RS04530 - ABC transporter permease, at 862,626 to 863,819
_RS04530
Position (kb)
861
862
863 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 860.703 kb on - strand at 860.704 kb on + strand at 860.716 kb on + strand at 860.717 kb on - strand at 860.766 kb on - strand at 860.795 kb on - strand at 860.796 kb on + strand at 860.819 kb on + strand at 860.837 kb on + strand at 860.837 kb on + strand at 860.848 kb on - strand at 860.855 kb on - strand at 860.856 kb on + strand at 860.875 kb on - strand at 860.880 kb on - strand at 860.881 kb on + strand at 860.885 kb on + strand at 860.943 kb on + strand at 861.008 kb on + strand at 861.088 kb on - strand at 861.088 kb on - strand at 861.088 kb on - strand at 861.397 kb on - strand, within MMP_RS04520 at 861.423 kb on - strand, within MMP_RS04520 at 861.514 kb on - strand, within MMP_RS04520 at 861.599 kb on - strand at 861.642 kb on + strand at 861.715 kb on - strand at 861.715 kb on - strand at 861.716 kb on + strand at 861.740 kb on - strand, within MMP_RS04525 at 861.774 kb on - strand, within MMP_RS04525 at 861.775 kb on + strand, within MMP_RS04525 at 861.780 kb on - strand, within MMP_RS04525 at 861.781 kb on + strand, within MMP_RS04525 at 861.786 kb on + strand, within MMP_RS04525 at 861.786 kb on + strand, within MMP_RS04525 at 861.825 kb on + strand, within MMP_RS04525 at 861.829 kb on - strand, within MMP_RS04525 at 861.829 kb on - strand, within MMP_RS04525 at 861.829 kb on - strand, within MMP_RS04525 at 861.830 kb on + strand, within MMP_RS04525 at 861.840 kb on - strand, within MMP_RS04525 at 861.853 kb on - strand, within MMP_RS04525 at 861.894 kb on + strand, within MMP_RS04525 at 861.900 kb on + strand, within MMP_RS04525 at 861.945 kb on + strand, within MMP_RS04525 at 861.956 kb on - strand, within MMP_RS04525 at 861.956 kb on - strand, within MMP_RS04525 at 861.957 kb on + strand, within MMP_RS04525 at 861.985 kb on - strand, within MMP_RS04525 at 861.985 kb on - strand, within MMP_RS04525 at 861.986 kb on + strand, within MMP_RS04525 at 861.986 kb on + strand, within MMP_RS04525 at 861.987 kb on - strand, within MMP_RS04525 at 861.999 kb on + strand, within MMP_RS04525 at 862.145 kb on - strand, within MMP_RS04525 at 862.145 kb on - strand, within MMP_RS04525 at 862.145 kb on - strand, within MMP_RS04525 at 862.146 kb on + strand, within MMP_RS04525 at 862.146 kb on + strand, within MMP_RS04525 at 862.146 kb on + strand, within MMP_RS04525 at 862.152 kb on + strand, within MMP_RS04525 at 862.152 kb on + strand, within MMP_RS04525 at 862.446 kb on + strand, within MMP_RS04525 at 862.446 kb on + strand, within MMP_RS04525 at 862.456 kb on + strand, within MMP_RS04525 at 862.461 kb on - strand, within MMP_RS04525 at 862.461 kb on - strand, within MMP_RS04525 at 862.465 kb on - strand, within MMP_RS04525 at 862.465 kb on - strand, within MMP_RS04525 at 862.465 kb on - strand, within MMP_RS04525 at 862.465 kb on - strand, within MMP_RS04525 at 862.466 kb on + strand, within MMP_RS04525 at 862.466 kb on + strand, within MMP_RS04525 at 862.466 kb on + strand, within MMP_RS04525 at 862.467 kb on - strand, within MMP_RS04525 at 862.467 kb on - strand, within MMP_RS04525 at 862.467 kb on - strand, within MMP_RS04525 at 862.467 kb on - strand, within MMP_RS04525 at 862.468 kb on + strand, within MMP_RS04525 at 862.468 kb on + strand, within MMP_RS04525 at 862.468 kb on + strand, within MMP_RS04525 at 862.470 kb on + strand, within MMP_RS04525 at 862.470 kb on + strand, within MMP_RS04525 at 862.470 kb on + strand, within MMP_RS04525 at 862.470 kb on + strand, within MMP_RS04525 at 862.564 kb on + strand at 862.566 kb on + strand at 862.578 kb on - strand at 862.585 kb on - strand at 862.589 kb on - strand at 862.594 kb on + strand at 862.594 kb on + strand at 862.667 kb on + strand at 862.693 kb on - strand at 862.714 kb on - strand at 862.714 kb on - strand at 862.715 kb on + strand at 862.715 kb on + strand at 862.718 kb on - strand at 862.767 kb on - strand, within MMP_RS04530 at 862.771 kb on - strand, within MMP_RS04530 at 862.771 kb on - strand, within MMP_RS04530 at 862.771 kb on - strand, within MMP_RS04530 at 862.883 kb on + strand, within MMP_RS04530 at 862.976 kb on + strand, within MMP_RS04530 at 863.006 kb on + strand, within MMP_RS04530 at 863.263 kb on + strand, within MMP_RS04530 at 863.263 kb on + strand, within MMP_RS04530 at 863.267 kb on - strand, within MMP_RS04530 at 863.309 kb on + strand, within MMP_RS04530 at 863.510 kb on - strand, within MMP_RS04530
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_H2_complete Irgasan 0.025 mM remove 860,703 - +0.3 860,704 + +1.0 860,716 + +0.2 860,717 - -0.1 860,766 - -0.1 860,795 - +1.2 860,796 + -3.7 860,819 + +0.7 860,837 + +0.3 860,837 + +0.5 860,848 - +0.1 860,855 - -1.1 860,856 + -0.0 860,875 - -1.9 860,880 - +0.9 860,881 + +1.4 860,885 + -0.6 860,943 + -1.0 861,008 + +0.3 861,088 - -0.6 861,088 - -0.6 861,088 - -0.1 861,397 - MMP_RS04520 0.54 +1.1 861,423 - MMP_RS04520 0.60 +0.7 861,514 - MMP_RS04520 0.81 +0.2 861,599 - -0.2 861,642 + +1.0 861,715 - -2.6 861,715 - -1.0 861,716 + -4.5 861,740 - MMP_RS04525 0.12 -0.2 861,774 - MMP_RS04525 0.15 +0.2 861,775 + MMP_RS04525 0.15 -0.6 861,780 - MMP_RS04525 0.16 +0.1 861,781 + MMP_RS04525 0.16 +0.2 861,786 + MMP_RS04525 0.17 -0.5 861,786 + MMP_RS04525 0.17 +0.4 861,825 + MMP_RS04525 0.21 +0.4 861,829 - MMP_RS04525 0.21 +0.3 861,829 - MMP_RS04525 0.21 -1.4 861,829 - MMP_RS04525 0.21 -1.1 861,830 + MMP_RS04525 0.21 -0.4 861,840 - MMP_RS04525 0.22 +1.3 861,853 - MMP_RS04525 0.24 -1.8 861,894 + MMP_RS04525 0.28 -1.2 861,900 + MMP_RS04525 0.28 +0.4 861,945 + MMP_RS04525 0.33 -0.9 861,956 - MMP_RS04525 0.34 -2.1 861,956 - MMP_RS04525 0.34 -0.2 861,957 + MMP_RS04525 0.34 -0.6 861,985 - MMP_RS04525 0.37 +0.9 861,985 - MMP_RS04525 0.37 +0.2 861,986 + MMP_RS04525 0.37 -1.8 861,986 + MMP_RS04525 0.37 +1.9 861,987 - MMP_RS04525 0.38 +0.2 861,999 + MMP_RS04525 0.39 -2.6 862,145 - MMP_RS04525 0.54 +0.8 862,145 - MMP_RS04525 0.54 -0.4 862,145 - MMP_RS04525 0.54 +0.6 862,146 + MMP_RS04525 0.54 +0.7 862,146 + MMP_RS04525 0.54 +0.4 862,146 + MMP_RS04525 0.54 -0.5 862,152 + MMP_RS04525 0.55 +2.0 862,152 + MMP_RS04525 0.55 -0.1 862,446 + MMP_RS04525 0.85 -1.3 862,446 + MMP_RS04525 0.85 +1.6 862,456 + MMP_RS04525 0.87 -0.8 862,461 - MMP_RS04525 0.87 -0.2 862,461 - MMP_RS04525 0.87 -0.1 862,465 - MMP_RS04525 0.87 -1.2 862,465 - MMP_RS04525 0.87 -1.8 862,465 - MMP_RS04525 0.87 +1.3 862,465 - MMP_RS04525 0.87 +0.4 862,466 + MMP_RS04525 0.88 -0.1 862,466 + MMP_RS04525 0.88 -2.7 862,466 + MMP_RS04525 0.88 -0.1 862,467 - MMP_RS04525 0.88 +2.8 862,467 - MMP_RS04525 0.88 -2.3 862,467 - MMP_RS04525 0.88 +0.4 862,467 - MMP_RS04525 0.88 -0.2 862,468 + MMP_RS04525 0.88 +0.8 862,468 + MMP_RS04525 0.88 +1.1 862,468 + MMP_RS04525 0.88 -1.1 862,470 + MMP_RS04525 0.88 +1.0 862,470 + MMP_RS04525 0.88 +0.2 862,470 + MMP_RS04525 0.88 -0.1 862,470 + MMP_RS04525 0.88 -0.2 862,564 + -0.6 862,566 + +0.1 862,578 - +1.2 862,585 - -0.9 862,589 - +0.2 862,594 + -0.7 862,594 + +0.2 862,667 + -0.8 862,693 - -0.7 862,714 - +0.0 862,714 - -0.8 862,715 + -0.9 862,715 + -0.2 862,718 - -0.4 862,767 - MMP_RS04530 0.12 -1.1 862,771 - MMP_RS04530 0.12 +0.3 862,771 - MMP_RS04530 0.12 -1.7 862,771 - MMP_RS04530 0.12 -0.0 862,883 + MMP_RS04530 0.22 -0.1 862,976 + MMP_RS04530 0.29 +0.3 863,006 + MMP_RS04530 0.32 -2.7 863,263 + MMP_RS04530 0.53 -0.0 863,263 + MMP_RS04530 0.53 +0.6 863,267 - MMP_RS04530 0.54 -0.1 863,309 + MMP_RS04530 0.57 +0.0 863,510 - MMP_RS04530 0.74 +0.9
Or see this region's nucleotide sequence