Experiment: McCas_H2_complete perchlorate 250 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02005 and MMP_RS02010 are separated by 5 nucleotides MMP_RS02010 and MMP_RS02015 are separated by 28 nucleotides MMP_RS02015 and MMP_RS02020 are separated by 5 nucleotides MMP_RS02020 and MMP_RS02025 are separated by 4 nucleotides
MMP_RS02005: MMP_RS02005 - RNA ligase, at 373,010 to 374,194
_RS02005
MMP_RS02010: MMP_RS02010 - PHP domain-containing protein, at 374,200 to 374,844
_RS02010
MMP_RS02015: MMP_RS02015 - amidohydrolase, at 374,873 to 376,156
_RS02015
MMP_RS02020: MMP_RS02020 - gamma-glutamylcyclotransferase, at 376,162 to 376,485
_RS02020
MMP_RS02025: MMP_RS02025 - DNA-directed DNA polymerase, at 376,490 to 378,844
_RS02025
Position (kb) 374
375
376
377 Strain fitness (log2 ratio) -1
0
1
2
3
4 at 374.061 kb on - strand, within MMP_RS02005 at 374.141 kb on - strand at 374.181 kb on - strand at 374.472 kb on - strand, within MMP_RS02010 at 374.474 kb on - strand, within MMP_RS02010 at 374.475 kb on + strand, within MMP_RS02010 at 374.476 kb on - strand, within MMP_RS02010 at 374.477 kb on + strand, within MMP_RS02010 at 374.485 kb on - strand, within MMP_RS02010 at 374.487 kb on - strand, within MMP_RS02010 at 374.492 kb on + strand, within MMP_RS02010 at 374.532 kb on - strand, within MMP_RS02010 at 374.576 kb on - strand, within MMP_RS02010 at 374.577 kb on + strand, within MMP_RS02010 at 374.599 kb on - strand, within MMP_RS02010 at 374.599 kb on - strand, within MMP_RS02010 at 374.698 kb on - strand, within MMP_RS02010 at 374.869 kb on - strand at 374.875 kb on + strand at 374.902 kb on - strand at 374.914 kb on - strand at 374.918 kb on + strand at 374.921 kb on - strand at 374.943 kb on - strand at 374.963 kb on - strand at 374.965 kb on - strand at 374.982 kb on + strand at 375.019 kb on - strand, within MMP_RS02015 at 375.038 kb on + strand, within MMP_RS02015 at 375.074 kb on - strand, within MMP_RS02015 at 375.077 kb on + strand, within MMP_RS02015 at 375.479 kb on - strand, within MMP_RS02015 at 375.480 kb on + strand, within MMP_RS02015 at 375.518 kb on - strand, within MMP_RS02015 at 375.626 kb on + strand, within MMP_RS02015 at 375.665 kb on - strand, within MMP_RS02015 at 375.667 kb on - strand, within MMP_RS02015 at 375.835 kb on + strand, within MMP_RS02015 at 376.049 kb on + strand at 376.127 kb on + strand at 376.131 kb on - strand at 376.132 kb on + strand at 376.179 kb on + strand at 376.221 kb on - strand, within MMP_RS02020 at 376.300 kb on - strand, within MMP_RS02020 at 376.318 kb on + strand, within MMP_RS02020 at 376.385 kb on - strand, within MMP_RS02020 at 376.479 kb on + strand at 376.568 kb on - strand at 376.679 kb on - strand at 376.682 kb on + strand at 376.682 kb on + strand at 376.691 kb on - strand at 376.691 kb on - strand at 376.691 kb on - strand at 376.730 kb on - strand, within MMP_RS02025 at 376.768 kb on - strand, within MMP_RS02025 at 376.789 kb on - strand, within MMP_RS02025 at 376.974 kb on + strand, within MMP_RS02025
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_H2_complete perchlorate 250 mM remove 374,061 - MMP_RS02005 0.89 -1.3 374,141 - +0.8 374,181 - -0.7 374,472 - MMP_RS02010 0.42 -0.5 374,474 - MMP_RS02010 0.42 -1.5 374,475 + MMP_RS02010 0.43 -1.2 374,476 - MMP_RS02010 0.43 +1.1 374,477 + MMP_RS02010 0.43 +0.5 374,485 - MMP_RS02010 0.44 +1.5 374,487 - MMP_RS02010 0.44 +2.4 374,492 + MMP_RS02010 0.45 +0.4 374,532 - MMP_RS02010 0.51 +3.4 374,576 - MMP_RS02010 0.58 +2.7 374,577 + MMP_RS02010 0.58 +1.7 374,599 - MMP_RS02010 0.62 +0.2 374,599 - MMP_RS02010 0.62 +1.6 374,698 - MMP_RS02010 0.77 +2.8 374,869 - +1.2 374,875 + +4.2 374,902 - -0.5 374,914 - +1.1 374,918 + +2.4 374,921 - +1.3 374,943 - +0.3 374,963 - +2.2 374,965 - +0.4 374,982 + -1.2 375,019 - MMP_RS02015 0.11 +0.0 375,038 + MMP_RS02015 0.13 +0.6 375,074 - MMP_RS02015 0.16 +0.0 375,077 + MMP_RS02015 0.16 -1.5 375,479 - MMP_RS02015 0.47 -0.8 375,480 + MMP_RS02015 0.47 -0.6 375,518 - MMP_RS02015 0.50 -0.7 375,626 + MMP_RS02015 0.59 -0.1 375,665 - MMP_RS02015 0.62 -1.4 375,667 - MMP_RS02015 0.62 +0.1 375,835 + MMP_RS02015 0.75 -0.5 376,049 + +1.0 376,127 + -0.9 376,131 - -1.4 376,132 + +1.6 376,179 + -0.7 376,221 - MMP_RS02020 0.18 +0.4 376,300 - MMP_RS02020 0.43 -0.1 376,318 + MMP_RS02020 0.48 -0.9 376,385 - MMP_RS02020 0.69 +0.3 376,479 + +0.4 376,568 - +0.4 376,679 - +0.6 376,682 + +0.5 376,682 + -1.5 376,691 - -0.0 376,691 - +0.1 376,691 - -0.5 376,730 - MMP_RS02025 0.10 -1.2 376,768 - MMP_RS02025 0.12 +0.0 376,789 - MMP_RS02025 0.13 +0.6 376,974 + MMP_RS02025 0.21 +0.3
Or see this region's nucleotide sequence