Experiment: MoLS4 with L-Tryptophan as nutrient source 10mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt bioA and DVU2560 overlap by 4 nucleotides DVU2560 and DVU2561 overlap by 4 nucleotides DVU2561 and acpP are separated by 99 nucleotides acpP and fabF are separated by 277 nucleotides
DVU2559: bioA - adenosylmethionine--8-amino-7-oxononanoate aminotransferase (TIGR), at 2,670,824 to 2,672,452
bioA
DVU2560: DVU2560 - conserved domain protein (TIGR), at 2,672,449 to 2,672,835
DVU2560
DVU2561: DVU2561 - oxidoreductase, short chain dehydrogenase/reductase family (TIGR), at 2,672,832 to 2,673,860
DVU2561
DVU2562: acpP - acyl carrier protein (TIGR), at 2,673,960 to 2,674,367
acpP
DVU2563: fabF - beta-ketoacyl synthase, putative (TIGR), at 2,674,645 to 2,676,093
fabF
Position (kb)
2672
2673
2674 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2671.838 kb on + strand, within bioA at 2671.939 kb on + strand, within bioA at 2671.951 kb on + strand, within bioA at 2671.961 kb on - strand, within bioA at 2671.967 kb on + strand, within bioA at 2671.967 kb on + strand, within bioA at 2671.969 kb on - strand, within bioA at 2671.975 kb on - strand, within bioA at 2671.995 kb on + strand, within bioA at 2672.071 kb on - strand, within bioA at 2672.117 kb on - strand, within bioA at 2672.127 kb on + strand, within bioA at 2672.127 kb on + strand, within bioA at 2672.140 kb on - strand, within bioA at 2672.160 kb on + strand, within bioA at 2672.285 kb on + strand, within bioA at 2672.285 kb on + strand, within bioA at 2672.293 kb on - strand at 2672.396 kb on - strand at 2672.422 kb on - strand at 2672.456 kb on - strand at 2672.509 kb on - strand, within DVU2560 at 2672.519 kb on - strand, within DVU2560 at 2672.576 kb on - strand, within DVU2560 at 2672.662 kb on + strand, within DVU2560 at 2672.672 kb on + strand, within DVU2560 at 2672.674 kb on - strand, within DVU2560 at 2672.684 kb on + strand, within DVU2560 at 2672.695 kb on - strand, within DVU2560 at 2672.702 kb on - strand, within DVU2560 at 2672.728 kb on + strand, within DVU2560 at 2672.830 kb on + strand at 2672.853 kb on + strand at 2672.904 kb on + strand at 2672.906 kb on + strand at 2672.914 kb on - strand at 2672.914 kb on - strand at 2672.931 kb on - strand at 2672.953 kb on + strand, within DVU2561 at 2673.041 kb on - strand, within DVU2561 at 2673.077 kb on - strand, within DVU2561 at 2673.106 kb on + strand, within DVU2561 at 2673.114 kb on - strand, within DVU2561 at 2673.124 kb on - strand, within DVU2561 at 2673.127 kb on + strand, within DVU2561 at 2673.127 kb on + strand, within DVU2561 at 2673.127 kb on + strand, within DVU2561 at 2673.127 kb on + strand, within DVU2561 at 2673.135 kb on - strand, within DVU2561 at 2673.135 kb on - strand, within DVU2561 at 2673.135 kb on - strand, within DVU2561 at 2673.135 kb on - strand, within DVU2561 at 2673.145 kb on - strand, within DVU2561 at 2673.153 kb on + strand, within DVU2561 at 2673.165 kb on + strand, within DVU2561 at 2673.197 kb on - strand, within DVU2561 at 2673.301 kb on - strand, within DVU2561 at 2673.454 kb on - strand, within DVU2561 at 2673.463 kb on + strand, within DVU2561 at 2673.501 kb on + strand, within DVU2561 at 2673.501 kb on + strand, within DVU2561 at 2673.506 kb on + strand, within DVU2561 at 2673.506 kb on + strand, within DVU2561 at 2673.512 kb on - strand, within DVU2561 at 2673.528 kb on - strand, within DVU2561 at 2673.605 kb on + strand, within DVU2561 at 2673.622 kb on + strand, within DVU2561 at 2673.622 kb on + strand, within DVU2561 at 2673.743 kb on + strand, within DVU2561 at 2673.894 kb on + strand at 2673.895 kb on - strand at 2673.900 kb on - strand at 2673.900 kb on - strand at 2673.902 kb on - strand at 2673.983 kb on - strand at 2674.059 kb on + strand, within acpP at 2674.109 kb on - strand, within acpP at 2674.109 kb on - strand, within acpP at 2674.143 kb on + strand, within acpP at 2674.186 kb on - strand, within acpP at 2674.240 kb on - strand, within acpP at 2674.304 kb on + strand, within acpP at 2674.344 kb on + strand at 2674.349 kb on + strand at 2674.378 kb on - strand at 2674.394 kb on + strand at 2674.427 kb on + strand at 2674.429 kb on + strand at 2674.471 kb on + strand at 2674.531 kb on + strand at 2674.539 kb on - strand at 2674.541 kb on + strand at 2674.541 kb on + strand at 2674.544 kb on - strand at 2674.546 kb on + strand at 2674.549 kb on - strand at 2674.642 kb on + strand at 2674.715 kb on - strand at 2674.732 kb on + strand at 2674.754 kb on - strand at 2674.785 kb on + strand at 2674.793 kb on - strand, within fabF at 2674.806 kb on + strand, within fabF
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Tryptophan as nutrient source 10mM remove 2,671,838 + bioA DVU2559 0.62 -1.0 2,671,939 + bioA DVU2559 0.68 +0.5 2,671,951 + bioA DVU2559 0.69 -1.0 2,671,961 - bioA DVU2559 0.70 -1.2 2,671,967 + bioA DVU2559 0.70 +0.2 2,671,967 + bioA DVU2559 0.70 -1.2 2,671,969 - bioA DVU2559 0.70 -0.9 2,671,975 - bioA DVU2559 0.71 +0.3 2,671,995 + bioA DVU2559 0.72 -0.7 2,672,071 - bioA DVU2559 0.77 -1.6 2,672,117 - bioA DVU2559 0.79 -0.8 2,672,127 + bioA DVU2559 0.80 +3.0 2,672,127 + bioA DVU2559 0.80 +2.4 2,672,140 - bioA DVU2559 0.81 -1.2 2,672,160 + bioA DVU2559 0.82 -2.0 2,672,285 + bioA DVU2559 0.90 +3.0 2,672,285 + bioA DVU2559 0.90 -0.9 2,672,293 - -0.7 2,672,396 - -0.8 2,672,422 - -0.6 2,672,456 - -0.7 2,672,509 - DVU2560 0.16 -1.0 2,672,519 - DVU2560 0.18 -1.4 2,672,576 - DVU2560 0.33 -1.9 2,672,662 + DVU2560 0.55 -0.4 2,672,672 + DVU2560 0.58 -1.1 2,672,674 - DVU2560 0.58 +1.1 2,672,684 + DVU2560 0.61 -1.5 2,672,695 - DVU2560 0.64 -0.9 2,672,702 - DVU2560 0.65 -2.8 2,672,728 + DVU2560 0.72 +1.2 2,672,830 + -1.2 2,672,853 + -2.0 2,672,904 + +2.4 2,672,906 + -0.9 2,672,914 - +1.2 2,672,914 - -0.9 2,672,931 - -1.5 2,672,953 + DVU2561 0.12 -0.4 2,673,041 - DVU2561 0.20 +0.2 2,673,077 - DVU2561 0.24 -0.1 2,673,106 + DVU2561 0.27 -1.2 2,673,114 - DVU2561 0.27 -0.1 2,673,124 - DVU2561 0.28 -0.7 2,673,127 + DVU2561 0.29 -1.6 2,673,127 + DVU2561 0.29 -1.3 2,673,127 + DVU2561 0.29 -1.7 2,673,127 + DVU2561 0.29 -0.4 2,673,135 - DVU2561 0.29 -0.8 2,673,135 - DVU2561 0.29 -2.5 2,673,135 - DVU2561 0.29 -0.9 2,673,135 - DVU2561 0.29 -1.3 2,673,145 - DVU2561 0.30 -0.6 2,673,153 + DVU2561 0.31 -3.1 2,673,165 + DVU2561 0.32 -2.1 2,673,197 - DVU2561 0.35 -1.9 2,673,301 - DVU2561 0.46 +0.2 2,673,454 - DVU2561 0.60 -1.5 2,673,463 + DVU2561 0.61 -1.7 2,673,501 + DVU2561 0.65 -1.7 2,673,501 + DVU2561 0.65 -0.7 2,673,506 + DVU2561 0.66 +0.1 2,673,506 + DVU2561 0.66 -1.2 2,673,512 - DVU2561 0.66 -1.2 2,673,528 - DVU2561 0.68 +4.0 2,673,605 + DVU2561 0.75 -0.5 2,673,622 + DVU2561 0.77 -0.9 2,673,622 + DVU2561 0.77 -1.4 2,673,743 + DVU2561 0.89 -1.4 2,673,894 + -0.2 2,673,895 - -0.6 2,673,900 - -0.5 2,673,900 - -1.1 2,673,902 - -0.7 2,673,983 - -1.6 2,674,059 + acpP DVU2562 0.24 +1.2 2,674,109 - acpP DVU2562 0.37 +0.3 2,674,109 - acpP DVU2562 0.37 -1.4 2,674,143 + acpP DVU2562 0.45 -1.1 2,674,186 - acpP DVU2562 0.55 -0.7 2,674,240 - acpP DVU2562 0.69 -2.4 2,674,304 + acpP DVU2562 0.84 -0.9 2,674,344 + +0.5 2,674,349 + +2.0 2,674,378 - -0.7 2,674,394 + +0.2 2,674,427 + +0.8 2,674,429 + +2.4 2,674,471 + +0.1 2,674,531 + -0.1 2,674,539 - -1.5 2,674,541 + -0.9 2,674,541 + -0.3 2,674,544 - -1.1 2,674,546 + -0.2 2,674,549 - +0.3 2,674,642 + -0.9 2,674,715 - +0.3 2,674,732 + +0.1 2,674,754 - -1.1 2,674,785 + -3.3 2,674,793 - fabF DVU2563 0.10 -1.4 2,674,806 + fabF DVU2563 0.11 -0.7
Or see this region's nucleotide sequence