Experiment: KB with Gambogic Acid 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2848 and Psyr_2849 are separated by 515 nucleotides Psyr_2849 and Psyr_2850 are separated by 82 nucleotides Psyr_2850 and Psyr_2851 are separated by 81 nucleotides
Psyr_2848: Psyr_2848 - hypothetical protein, at 3,421,497 to 3,421,922
_2848
Psyr_2849: Psyr_2849 - CDS, at 3,422,438 to 3,423,085
_2849
Psyr_2850: Psyr_2850 - hypothetical protein, at 3,423,168 to 3,423,527
_2850
Psyr_2851: Psyr_2851 - conserved hypothetical protein, at 3,423,609 to 3,423,962
_2851
Position (kb)
3422
3423
3424 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3421.438 kb on + strand at 3421.442 kb on + strand at 3421.443 kb on - strand at 3421.469 kb on + strand at 3421.469 kb on + strand at 3421.469 kb on + strand at 3421.470 kb on - strand at 3421.470 kb on - strand at 3421.533 kb on + strand at 3421.557 kb on + strand, within Psyr_2848 at 3421.558 kb on - strand, within Psyr_2848 at 3421.558 kb on - strand, within Psyr_2848 at 3421.622 kb on - strand, within Psyr_2848 at 3421.653 kb on - strand, within Psyr_2848 at 3421.696 kb on - strand, within Psyr_2848 at 3421.797 kb on + strand, within Psyr_2848 at 3421.797 kb on + strand, within Psyr_2848 at 3421.797 kb on + strand, within Psyr_2848 at 3421.798 kb on - strand, within Psyr_2848 at 3421.798 kb on - strand, within Psyr_2848 at 3421.893 kb on - strand at 3421.910 kb on - strand at 3421.924 kb on - strand at 3421.928 kb on - strand at 3421.955 kb on + strand at 3421.955 kb on + strand at 3421.955 kb on + strand at 3421.955 kb on + strand at 3421.955 kb on + strand at 3421.955 kb on + strand at 3421.955 kb on + strand at 3421.955 kb on + strand at 3421.955 kb on + strand at 3421.955 kb on + strand at 3421.956 kb on - strand at 3421.956 kb on - strand at 3421.956 kb on - strand at 3421.956 kb on - strand at 3421.956 kb on - strand at 3421.956 kb on - strand at 3421.956 kb on - strand at 3421.995 kb on + strand at 3421.997 kb on + strand at 3421.997 kb on + strand at 3421.997 kb on + strand at 3421.997 kb on + strand at 3422.001 kb on + strand at 3422.002 kb on - strand at 3422.002 kb on - strand at 3422.005 kb on + strand at 3422.099 kb on + strand at 3422.104 kb on - strand at 3422.185 kb on + strand at 3422.185 kb on + strand at 3422.185 kb on + strand at 3422.186 kb on - strand at 3422.186 kb on - strand at 3422.186 kb on - strand at 3422.186 kb on - strand at 3422.205 kb on + strand at 3422.205 kb on + strand at 3422.205 kb on + strand at 3422.205 kb on + strand at 3422.206 kb on - strand at 3422.206 kb on - strand at 3422.206 kb on - strand at 3422.367 kb on + strand at 3422.367 kb on + strand at 3422.367 kb on + strand at 3422.367 kb on + strand at 3422.367 kb on + strand at 3422.367 kb on + strand at 3422.367 kb on + strand at 3422.367 kb on + strand at 3422.367 kb on + strand at 3422.367 kb on + strand at 3422.367 kb on + strand at 3422.367 kb on + strand at 3422.367 kb on + strand at 3422.368 kb on - strand at 3422.368 kb on - strand at 3422.368 kb on - strand at 3422.368 kb on - strand at 3422.368 kb on - strand at 3422.368 kb on - strand at 3422.368 kb on - strand at 3422.368 kb on - strand at 3422.368 kb on - strand at 3422.373 kb on + strand at 3422.373 kb on + strand at 3422.373 kb on + strand at 3422.373 kb on + strand at 3423.114 kb on + strand at 3423.146 kb on + strand at 3423.146 kb on + strand at 3423.184 kb on + strand at 3423.184 kb on + strand at 3423.184 kb on + strand at 3423.184 kb on + strand at 3423.184 kb on + strand at 3423.184 kb on + strand at 3423.184 kb on + strand at 3423.185 kb on - strand at 3423.185 kb on - strand at 3423.185 kb on - strand at 3423.185 kb on - strand at 3423.185 kb on - strand at 3423.185 kb on - strand at 3423.189 kb on - strand at 3423.189 kb on - strand at 3423.236 kb on - strand, within Psyr_2850 at 3423.337 kb on - strand, within Psyr_2850 at 3423.337 kb on - strand, within Psyr_2850 at 3423.337 kb on - strand, within Psyr_2850 at 3423.360 kb on + strand, within Psyr_2850 at 3423.360 kb on + strand, within Psyr_2850 at 3423.360 kb on + strand, within Psyr_2850 at 3423.360 kb on + strand, within Psyr_2850 at 3423.361 kb on - strand, within Psyr_2850 at 3423.361 kb on - strand, within Psyr_2850 at 3423.492 kb on + strand at 3423.594 kb on + strand at 3423.607 kb on + strand at 3423.826 kb on - strand, within Psyr_2851 at 3423.845 kb on - strand, within Psyr_2851 at 3423.873 kb on + strand, within Psyr_2851 at 3423.874 kb on - strand, within Psyr_2851 at 3423.993 kb on + strand at 3423.993 kb on + strand at 3424.083 kb on - strand at 3424.083 kb on - strand at 3424.083 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Gambogic Acid 10 mM remove 3,421,438 + +0.2 3,421,442 + -3.5 3,421,443 - -0.2 3,421,469 + +0.6 3,421,469 + -0.8 3,421,469 + -1.5 3,421,470 - -1.2 3,421,470 - -0.9 3,421,533 + +0.6 3,421,557 + Psyr_2848 0.14 -3.0 3,421,558 - Psyr_2848 0.14 -1.8 3,421,558 - Psyr_2848 0.14 -2.2 3,421,622 - Psyr_2848 0.29 -1.8 3,421,653 - Psyr_2848 0.37 +0.9 3,421,696 - Psyr_2848 0.47 +0.2 3,421,797 + Psyr_2848 0.70 -0.8 3,421,797 + Psyr_2848 0.70 -3.1 3,421,797 + Psyr_2848 0.70 -2.9 3,421,798 - Psyr_2848 0.71 -0.5 3,421,798 - Psyr_2848 0.71 -1.3 3,421,893 - +1.2 3,421,910 - -1.8 3,421,924 - +1.2 3,421,928 - +0.2 3,421,955 + -1.0 3,421,955 + -0.2 3,421,955 + +0.1 3,421,955 + +0.8 3,421,955 + +0.9 3,421,955 + -0.7 3,421,955 + +0.2 3,421,955 + +1.0 3,421,955 + -1.8 3,421,955 + -1.6 3,421,956 - -0.3 3,421,956 - -0.4 3,421,956 - +1.0 3,421,956 - -0.2 3,421,956 - +0.5 3,421,956 - +0.4 3,421,956 - +1.2 3,421,995 + -0.4 3,421,997 + -0.3 3,421,997 + -0.3 3,421,997 + +0.9 3,421,997 + -0.7 3,422,001 + -0.3 3,422,002 - -0.2 3,422,002 - +0.2 3,422,005 + -0.1 3,422,099 + -1.5 3,422,104 - -1.2 3,422,185 + +0.2 3,422,185 + +0.6 3,422,185 + -0.2 3,422,186 - -0.8 3,422,186 - -1.2 3,422,186 - +0.0 3,422,186 - -1.1 3,422,205 + -1.0 3,422,205 + +0.8 3,422,205 + -1.8 3,422,205 + -0.4 3,422,206 - +0.3 3,422,206 - +0.3 3,422,206 - +0.4 3,422,367 + -0.7 3,422,367 + -1.2 3,422,367 + +0.0 3,422,367 + -0.4 3,422,367 + +0.6 3,422,367 + -0.0 3,422,367 + -0.7 3,422,367 + +1.2 3,422,367 + -0.8 3,422,367 + +2.4 3,422,367 + +1.0 3,422,367 + -0.1 3,422,367 + +1.2 3,422,368 - +0.2 3,422,368 - -0.5 3,422,368 - -0.0 3,422,368 - -1.1 3,422,368 - +0.4 3,422,368 - +0.4 3,422,368 - -1.7 3,422,368 - -1.3 3,422,368 - +0.5 3,422,373 + +0.1 3,422,373 + +1.2 3,422,373 + -0.7 3,422,373 + +2.1 3,423,114 + -0.4 3,423,146 + -1.6 3,423,146 + +1.2 3,423,184 + +1.4 3,423,184 + +0.6 3,423,184 + +1.8 3,423,184 + -1.5 3,423,184 + -0.4 3,423,184 + -0.3 3,423,184 + -0.7 3,423,185 - -0.7 3,423,185 - -0.0 3,423,185 - -2.5 3,423,185 - +0.3 3,423,185 - -1.5 3,423,185 - +0.6 3,423,189 - +0.8 3,423,189 - +1.0 3,423,236 - Psyr_2850 0.19 -0.1 3,423,337 - Psyr_2850 0.47 -1.4 3,423,337 - Psyr_2850 0.47 +0.9 3,423,337 - Psyr_2850 0.47 -0.3 3,423,360 + Psyr_2850 0.53 +0.2 3,423,360 + Psyr_2850 0.53 -1.8 3,423,360 + Psyr_2850 0.53 -1.4 3,423,360 + Psyr_2850 0.53 -0.6 3,423,361 - Psyr_2850 0.54 -0.8 3,423,361 - Psyr_2850 0.54 +1.7 3,423,492 + +1.8 3,423,594 + -1.9 3,423,607 + +3.3 3,423,826 - Psyr_2851 0.61 +0.2 3,423,845 - Psyr_2851 0.67 +0.3 3,423,873 + Psyr_2851 0.75 -1.0 3,423,874 - Psyr_2851 0.75 +0.1 3,423,993 + +0.2 3,423,993 + -0.1 3,424,083 - -2.2 3,424,083 - +2.2 3,424,083 - -0.2
Or see this region's nucleotide sequence