Experiment: KB with Gambogic Acid 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2305 and Psyr_2306 are separated by 61 nucleotides Psyr_2306 and Psyr_2307 are separated by 6 nucleotides Psyr_2307 and Psyr_2308 are separated by 129 nucleotides Psyr_2308 and Psyr_2309 are separated by 63 nucleotides
Psyr_2305: Psyr_2305 - Natural resistance-associated macrophage protein, at 2,680,305 to 2,681,624
_2305
Psyr_2306: Psyr_2306 - General substrate transporter:Major facilitator superfamily, at 2,681,686 to 2,683,137
_2306
Psyr_2307: Psyr_2307 - conserved hypothetical protein, at 2,683,144 to 2,683,365
_2307
Psyr_2308: Psyr_2308 - conserved hypothetical protein, at 2,683,495 to 2,683,698
_2308
Psyr_2309: Psyr_2309 - conserved hypothetical protein, at 2,683,762 to 2,684,304
_2309
Position (kb)
2681
2682
2683
2684 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2680.822 kb on + strand, within Psyr_2305 at 2680.823 kb on - strand, within Psyr_2305 at 2680.823 kb on - strand, within Psyr_2305 at 2680.950 kb on + strand, within Psyr_2305 at 2680.950 kb on + strand, within Psyr_2305 at 2680.950 kb on + strand, within Psyr_2305 at 2680.950 kb on + strand, within Psyr_2305 at 2680.951 kb on - strand, within Psyr_2305 at 2680.992 kb on - strand, within Psyr_2305 at 2680.998 kb on + strand, within Psyr_2305 at 2680.998 kb on + strand, within Psyr_2305 at 2681.603 kb on - strand at 2681.603 kb on - strand at 2681.603 kb on - strand at 2681.911 kb on + strand, within Psyr_2306 at 2681.912 kb on - strand, within Psyr_2306 at 2681.912 kb on - strand, within Psyr_2306 at 2681.912 kb on - strand, within Psyr_2306 at 2681.912 kb on - strand, within Psyr_2306 at 2681.912 kb on - strand, within Psyr_2306 at 2681.913 kb on + strand, within Psyr_2306 at 2682.004 kb on + strand, within Psyr_2306 at 2682.004 kb on + strand, within Psyr_2306 at 2682.004 kb on + strand, within Psyr_2306 at 2682.004 kb on + strand, within Psyr_2306 at 2682.004 kb on + strand, within Psyr_2306 at 2682.005 kb on - strand, within Psyr_2306 at 2682.005 kb on - strand, within Psyr_2306 at 2682.005 kb on - strand, within Psyr_2306 at 2682.005 kb on - strand, within Psyr_2306 at 2682.042 kb on + strand, within Psyr_2306 at 2682.042 kb on + strand, within Psyr_2306 at 2682.043 kb on - strand, within Psyr_2306 at 2682.043 kb on - strand, within Psyr_2306 at 2682.043 kb on - strand, within Psyr_2306 at 2682.043 kb on - strand, within Psyr_2306 at 2682.097 kb on + strand, within Psyr_2306 at 2682.165 kb on + strand, within Psyr_2306 at 2682.166 kb on - strand, within Psyr_2306 at 2682.348 kb on - strand, within Psyr_2306 at 2682.389 kb on + strand, within Psyr_2306 at 2682.389 kb on + strand, within Psyr_2306 at 2682.419 kb on + strand, within Psyr_2306 at 2682.420 kb on - strand, within Psyr_2306 at 2682.420 kb on - strand, within Psyr_2306 at 2682.563 kb on - strand, within Psyr_2306 at 2682.584 kb on - strand, within Psyr_2306 at 2682.584 kb on - strand, within Psyr_2306 at 2682.584 kb on - strand, within Psyr_2306 at 2682.607 kb on + strand, within Psyr_2306 at 2682.607 kb on + strand, within Psyr_2306 at 2682.607 kb on + strand, within Psyr_2306 at 2682.607 kb on + strand, within Psyr_2306 at 2682.607 kb on + strand, within Psyr_2306 at 2682.607 kb on + strand, within Psyr_2306 at 2682.607 kb on + strand, within Psyr_2306 at 2682.607 kb on + strand, within Psyr_2306 at 2682.607 kb on + strand, within Psyr_2306 at 2682.607 kb on + strand, within Psyr_2306 at 2682.608 kb on - strand, within Psyr_2306 at 2682.608 kb on - strand, within Psyr_2306 at 2682.608 kb on - strand, within Psyr_2306 at 2682.608 kb on - strand, within Psyr_2306 at 2682.608 kb on - strand, within Psyr_2306 at 2682.608 kb on - strand, within Psyr_2306 at 2682.608 kb on - strand, within Psyr_2306 at 2682.638 kb on - strand, within Psyr_2306 at 2682.733 kb on + strand, within Psyr_2306 at 2682.734 kb on - strand, within Psyr_2306 at 2682.734 kb on - strand, within Psyr_2306 at 2682.734 kb on - strand, within Psyr_2306 at 2682.734 kb on - strand, within Psyr_2306 at 2682.862 kb on + strand, within Psyr_2306 at 2682.863 kb on - strand, within Psyr_2306 at 2682.923 kb on - strand, within Psyr_2306 at 2683.244 kb on + strand, within Psyr_2307 at 2683.307 kb on + strand, within Psyr_2307 at 2683.307 kb on + strand, within Psyr_2307 at 2683.307 kb on + strand, within Psyr_2307 at 2683.307 kb on + strand, within Psyr_2307 at 2683.307 kb on + strand, within Psyr_2307 at 2683.307 kb on + strand, within Psyr_2307 at 2683.307 kb on + strand, within Psyr_2307 at 2683.307 kb on + strand, within Psyr_2307 at 2683.307 kb on + strand, within Psyr_2307 at 2683.308 kb on - strand, within Psyr_2307 at 2683.308 kb on - strand, within Psyr_2307 at 2683.308 kb on - strand, within Psyr_2307 at 2683.308 kb on - strand, within Psyr_2307 at 2683.308 kb on - strand, within Psyr_2307 at 2683.308 kb on - strand, within Psyr_2307 at 2683.356 kb on + strand at 2683.356 kb on + strand at 2683.356 kb on + strand at 2683.356 kb on + strand at 2683.356 kb on + strand at 2683.356 kb on + strand at 2683.356 kb on + strand at 2683.357 kb on - strand at 2683.357 kb on - strand at 2683.357 kb on - strand at 2683.357 kb on - strand at 2683.357 kb on - strand at 2683.357 kb on - strand at 2683.376 kb on + strand at 2683.377 kb on - strand at 2683.392 kb on + strand at 2683.392 kb on + strand at 2683.392 kb on + strand at 2683.393 kb on - strand at 2683.395 kb on + strand at 2683.453 kb on + strand at 2683.457 kb on + strand at 2683.457 kb on + strand at 2683.458 kb on - strand at 2683.458 kb on - strand at 2683.497 kb on - strand at 2683.675 kb on - strand, within Psyr_2308 at 2683.692 kb on + strand at 2683.692 kb on + strand at 2683.692 kb on + strand at 2683.692 kb on + strand at 2683.693 kb on - strand at 2683.928 kb on + strand, within Psyr_2309 at 2683.928 kb on + strand, within Psyr_2309 at 2683.928 kb on + strand, within Psyr_2309 at 2683.929 kb on - strand, within Psyr_2309 at 2683.929 kb on - strand, within Psyr_2309 at 2683.929 kb on - strand, within Psyr_2309 at 2683.929 kb on - strand, within Psyr_2309 at 2683.929 kb on - strand, within Psyr_2309 at 2684.102 kb on + strand, within Psyr_2309 at 2684.112 kb on + strand, within Psyr_2309 at 2684.129 kb on + strand, within Psyr_2309
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Gambogic Acid 10 mM remove 2,680,822 + Psyr_2305 0.39 +0.2 2,680,823 - Psyr_2305 0.39 -0.1 2,680,823 - Psyr_2305 0.39 -0.5 2,680,950 + Psyr_2305 0.49 -0.3 2,680,950 + Psyr_2305 0.49 -1.2 2,680,950 + Psyr_2305 0.49 +0.7 2,680,950 + Psyr_2305 0.49 -0.3 2,680,951 - Psyr_2305 0.49 -0.8 2,680,992 - Psyr_2305 0.52 -0.6 2,680,998 + Psyr_2305 0.53 +0.7 2,680,998 + Psyr_2305 0.53 -0.4 2,681,603 - -2.2 2,681,603 - +0.6 2,681,603 - -0.0 2,681,911 + Psyr_2306 0.15 -1.7 2,681,912 - Psyr_2306 0.16 -0.3 2,681,912 - Psyr_2306 0.16 +0.8 2,681,912 - Psyr_2306 0.16 -0.3 2,681,912 - Psyr_2306 0.16 -1.8 2,681,912 - Psyr_2306 0.16 -1.3 2,681,913 + Psyr_2306 0.16 -2.2 2,682,004 + Psyr_2306 0.22 +0.5 2,682,004 + Psyr_2306 0.22 +0.9 2,682,004 + Psyr_2306 0.22 -0.1 2,682,004 + Psyr_2306 0.22 +1.6 2,682,004 + Psyr_2306 0.22 +0.3 2,682,005 - Psyr_2306 0.22 -1.9 2,682,005 - Psyr_2306 0.22 +0.6 2,682,005 - Psyr_2306 0.22 -0.4 2,682,005 - Psyr_2306 0.22 +0.1 2,682,042 + Psyr_2306 0.25 -1.8 2,682,042 + Psyr_2306 0.25 -2.3 2,682,043 - Psyr_2306 0.25 +0.6 2,682,043 - Psyr_2306 0.25 +1.2 2,682,043 - Psyr_2306 0.25 -0.2 2,682,043 - Psyr_2306 0.25 +0.8 2,682,097 + Psyr_2306 0.28 +0.4 2,682,165 + Psyr_2306 0.33 +0.3 2,682,166 - Psyr_2306 0.33 -0.2 2,682,348 - Psyr_2306 0.46 +1.9 2,682,389 + Psyr_2306 0.48 +1.2 2,682,389 + Psyr_2306 0.48 -0.4 2,682,419 + Psyr_2306 0.50 +0.4 2,682,420 - Psyr_2306 0.51 +1.5 2,682,420 - Psyr_2306 0.51 +2.9 2,682,563 - Psyr_2306 0.60 -0.7 2,682,584 - Psyr_2306 0.62 +0.3 2,682,584 - Psyr_2306 0.62 -0.4 2,682,584 - Psyr_2306 0.62 -0.6 2,682,607 + Psyr_2306 0.63 +0.6 2,682,607 + Psyr_2306 0.63 -2.0 2,682,607 + Psyr_2306 0.63 +1.3 2,682,607 + Psyr_2306 0.63 -0.2 2,682,607 + Psyr_2306 0.63 +0.6 2,682,607 + Psyr_2306 0.63 +0.3 2,682,607 + Psyr_2306 0.63 +0.2 2,682,607 + Psyr_2306 0.63 -1.8 2,682,607 + Psyr_2306 0.63 -1.9 2,682,607 + Psyr_2306 0.63 -0.4 2,682,608 - Psyr_2306 0.63 +1.2 2,682,608 - Psyr_2306 0.63 +1.3 2,682,608 - Psyr_2306 0.63 +1.2 2,682,608 - Psyr_2306 0.63 -0.3 2,682,608 - Psyr_2306 0.63 -1.2 2,682,608 - Psyr_2306 0.63 +2.2 2,682,608 - Psyr_2306 0.63 +0.2 2,682,638 - Psyr_2306 0.66 -0.7 2,682,733 + Psyr_2306 0.72 +0.8 2,682,734 - Psyr_2306 0.72 +0.9 2,682,734 - Psyr_2306 0.72 +1.4 2,682,734 - Psyr_2306 0.72 +0.0 2,682,734 - Psyr_2306 0.72 -0.1 2,682,862 + Psyr_2306 0.81 +1.1 2,682,863 - Psyr_2306 0.81 -1.6 2,682,923 - Psyr_2306 0.85 +0.3 2,683,244 + Psyr_2307 0.45 -0.2 2,683,307 + Psyr_2307 0.73 -1.7 2,683,307 + Psyr_2307 0.73 +1.0 2,683,307 + Psyr_2307 0.73 +2.2 2,683,307 + Psyr_2307 0.73 +1.2 2,683,307 + Psyr_2307 0.73 +0.3 2,683,307 + Psyr_2307 0.73 -0.8 2,683,307 + Psyr_2307 0.73 +1.9 2,683,307 + Psyr_2307 0.73 -0.1 2,683,307 + Psyr_2307 0.73 -0.8 2,683,308 - Psyr_2307 0.74 +0.7 2,683,308 - Psyr_2307 0.74 -0.4 2,683,308 - Psyr_2307 0.74 -1.4 2,683,308 - Psyr_2307 0.74 -1.5 2,683,308 - Psyr_2307 0.74 -0.2 2,683,308 - Psyr_2307 0.74 -0.3 2,683,356 + -0.0 2,683,356 + -1.8 2,683,356 + -1.0 2,683,356 + -1.9 2,683,356 + -1.4 2,683,356 + +0.8 2,683,356 + +1.2 2,683,357 - +1.2 2,683,357 - -1.5 2,683,357 - -1.8 2,683,357 - -1.8 2,683,357 - +1.0 2,683,357 - +0.6 2,683,376 + -0.7 2,683,377 - -1.2 2,683,392 + -1.4 2,683,392 + -0.5 2,683,392 + -1.3 2,683,393 - +0.3 2,683,395 + +0.5 2,683,453 + -2.7 2,683,457 + +0.0 2,683,457 + +0.1 2,683,458 - -0.3 2,683,458 - -2.5 2,683,497 - -1.2 2,683,675 - Psyr_2308 0.88 +1.1 2,683,692 + +0.9 2,683,692 + +0.4 2,683,692 + +0.9 2,683,692 + +0.2 2,683,693 - +1.8 2,683,928 + Psyr_2309 0.31 -1.3 2,683,928 + Psyr_2309 0.31 -0.6 2,683,928 + Psyr_2309 0.31 -1.5 2,683,929 - Psyr_2309 0.31 -0.6 2,683,929 - Psyr_2309 0.31 -0.2 2,683,929 - Psyr_2309 0.31 -0.2 2,683,929 - Psyr_2309 0.31 +0.2 2,683,929 - Psyr_2309 0.31 -0.4 2,684,102 + Psyr_2309 0.63 +2.2 2,684,112 + Psyr_2309 0.64 -0.7 2,684,129 + Psyr_2309 0.68 +0.3
Or see this region's nucleotide sequence