Strain Fitness in Pseudomonas syringae pv. syringae B728a around Psyr_1500

Experiment: KB with Gambogic Acid 10 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsyr_1498 and Psyr_1499 are separated by 69 nucleotidesPsyr_1499 and Psyr_1500 are separated by 35 nucleotidesPsyr_1500 and Psyr_1501 are separated by 29 nucleotidesPsyr_1501 and Psyr_1502 overlap by 1 nucleotides Psyr_1498: Psyr_1498 - Heavy metal sensor kinase, at 1,686,402 to 1,687,781 _1498 Psyr_1499: Psyr_1499 - Protein of unknown function DUF, at 1,687,851 to 1,688,312 _1499 Psyr_1500: Psyr_1500 - GCN5-related N-acetyltransferase, at 1,688,348 to 1,688,902 _1500 Psyr_1501: Psyr_1501 - NADPH-dependent FMN reductase, at 1,688,932 to 1,689,666 _1501 Psyr_1502: Psyr_1502 - Low molecular weight phosphotyrosine protein phosphatase, at 1,689,666 to 1,690,136 _1502 Position (kb) 1688 1689Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 1687.365 kb on + strand, within Psyr_1498at 1687.366 kb on - strand, within Psyr_1498at 1687.368 kb on + strand, within Psyr_1498at 1687.369 kb on - strand, within Psyr_1498at 1687.369 kb on - strand, within Psyr_1498at 1687.369 kb on - strand, within Psyr_1498at 1687.402 kb on + strand, within Psyr_1498at 1687.402 kb on + strand, within Psyr_1498at 1687.403 kb on - strand, within Psyr_1498at 1687.403 kb on - strand, within Psyr_1498at 1687.403 kb on - strand, within Psyr_1498at 1687.453 kb on + strand, within Psyr_1498at 1687.454 kb on + strand, within Psyr_1498at 1687.455 kb on - strand, within Psyr_1498at 1687.490 kb on + strand, within Psyr_1498at 1687.490 kb on + strand, within Psyr_1498at 1687.490 kb on + strand, within Psyr_1498at 1687.544 kb on - strand, within Psyr_1498at 1687.576 kb on + strand, within Psyr_1498at 1687.576 kb on + strand, within Psyr_1498at 1687.704 kb on - strandat 1687.706 kb on + strandat 1687.706 kb on + strandat 1687.707 kb on - strandat 1687.707 kb on - strandat 1687.707 kb on - strandat 1687.715 kb on + strandat 1687.715 kb on + strandat 1687.715 kb on + strandat 1687.715 kb on + strandat 1687.715 kb on + strandat 1687.715 kb on + strandat 1687.715 kb on + strandat 1687.715 kb on + strandat 1687.715 kb on + strandat 1687.715 kb on + strandat 1687.716 kb on - strandat 1687.716 kb on - strandat 1688.055 kb on + strand, within Psyr_1499at 1688.055 kb on + strand, within Psyr_1499at 1688.056 kb on - strand, within Psyr_1499at 1688.056 kb on - strand, within Psyr_1499at 1688.056 kb on - strand, within Psyr_1499at 1688.164 kb on + strand, within Psyr_1499at 1688.164 kb on + strand, within Psyr_1499at 1688.294 kb on + strandat 1688.295 kb on - strandat 1688.295 kb on - strandat 1688.295 kb on - strandat 1688.301 kb on + strandat 1688.301 kb on + strandat 1688.301 kb on + strandat 1688.301 kb on + strandat 1688.301 kb on + strandat 1688.302 kb on - strandat 1688.302 kb on - strandat 1688.302 kb on - strandat 1688.304 kb on - strandat 1688.326 kb on - strandat 1688.327 kb on + strandat 1688.327 kb on + strandat 1688.349 kb on + strandat 1688.349 kb on + strandat 1688.349 kb on + strandat 1688.350 kb on - strandat 1688.350 kb on - strandat 1688.350 kb on - strandat 1688.469 kb on + strand, within Psyr_1500at 1688.469 kb on + strand, within Psyr_1500at 1688.469 kb on + strand, within Psyr_1500at 1688.469 kb on + strand, within Psyr_1500at 1688.469 kb on + strand, within Psyr_1500at 1688.469 kb on + strand, within Psyr_1500at 1688.469 kb on + strand, within Psyr_1500at 1688.470 kb on - strand, within Psyr_1500at 1688.470 kb on - strand, within Psyr_1500at 1688.470 kb on - strand, within Psyr_1500at 1688.470 kb on - strand, within Psyr_1500at 1688.470 kb on - strand, within Psyr_1500at 1688.470 kb on - strand, within Psyr_1500at 1688.470 kb on - strand, within Psyr_1500at 1688.470 kb on - strand, within Psyr_1500at 1688.492 kb on + strand, within Psyr_1500at 1688.505 kb on - strand, within Psyr_1500at 1688.505 kb on - strand, within Psyr_1500at 1688.505 kb on - strand, within Psyr_1500at 1688.510 kb on + strand, within Psyr_1500at 1688.593 kb on - strand, within Psyr_1500at 1688.593 kb on - strand, within Psyr_1500at 1688.637 kb on + strand, within Psyr_1500at 1688.637 kb on + strand, within Psyr_1500at 1688.637 kb on + strand, within Psyr_1500at 1688.637 kb on + strand, within Psyr_1500at 1688.637 kb on + strand, within Psyr_1500at 1688.637 kb on + strandat 1688.637 kb on + strand, within Psyr_1500at 1688.637 kb on + strand, within Psyr_1500at 1688.638 kb on - strand, within Psyr_1500at 1688.638 kb on - strand, within Psyr_1500at 1688.638 kb on - strand, within Psyr_1500at 1688.638 kb on - strand, within Psyr_1500at 1688.638 kb on - strand, within Psyr_1500at 1688.638 kb on - strand, within Psyr_1500at 1688.638 kb on - strand, within Psyr_1500at 1688.638 kb on - strand, within Psyr_1500at 1688.638 kb on - strand, within Psyr_1500at 1688.638 kb on - strand, within Psyr_1500at 1688.664 kb on + strand, within Psyr_1500at 1688.664 kb on + strand, within Psyr_1500at 1688.700 kb on + strand, within Psyr_1500at 1688.700 kb on + strand, within Psyr_1500at 1688.742 kb on + strand, within Psyr_1500at 1688.838 kb on + strand, within Psyr_1500at 1688.838 kb on + strand, within Psyr_1500at 1688.838 kb on + strand, within Psyr_1500at 1688.838 kb on + strand, within Psyr_1500at 1688.838 kb on + strand, within Psyr_1500at 1688.838 kb on + strand, within Psyr_1500at 1688.838 kb on + strand, within Psyr_1500at 1688.838 kb on + strand, within Psyr_1500at 1688.838 kb on + strand, within Psyr_1500at 1688.838 kb on + strand, within Psyr_1500at 1688.838 kb on + strand, within Psyr_1500at 1688.838 kb on + strand, within Psyr_1500at 1688.838 kb on + strand, within Psyr_1500at 1688.839 kb on - strand, within Psyr_1500at 1688.839 kb on - strand, within Psyr_1500at 1688.839 kb on - strand, within Psyr_1500at 1688.839 kb on - strand, within Psyr_1500at 1688.839 kb on - strand, within Psyr_1500at 1688.839 kb on - strand, within Psyr_1500at 1688.839 kb on - strand, within Psyr_1500at 1688.839 kb on - strand, within Psyr_1500at 1688.839 kb on - strand, within Psyr_1500at 1688.839 kb on - strand, within Psyr_1500at 1688.839 kb on - strand, within Psyr_1500at 1688.839 kb on - strand, within Psyr_1500at 1688.839 kb on - strand, within Psyr_1500at 1688.842 kb on + strand, within Psyr_1500at 1688.842 kb on + strand, within Psyr_1500at 1688.921 kb on + strandat 1688.983 kb on - strandat 1689.002 kb on - strandat 1689.008 kb on + strand, within Psyr_1501at 1689.008 kb on + strand, within Psyr_1501at 1689.009 kb on - strand, within Psyr_1501at 1689.009 kb on - strand, within Psyr_1501at 1689.009 kb on - strand, within Psyr_1501at 1689.077 kb on + strand, within Psyr_1501at 1689.078 kb on - strand, within Psyr_1501at 1689.078 kb on - strand, within Psyr_1501at 1689.078 kb on - strand, within Psyr_1501at 1689.078 kb on - strand, within Psyr_1501at 1689.080 kb on + strand, within Psyr_1501at 1689.080 kb on + strand, within Psyr_1501at 1689.080 kb on + strand, within Psyr_1501at 1689.227 kb on - strand, within Psyr_1501at 1689.266 kb on - strand, within Psyr_1501at 1689.374 kb on - strand, within Psyr_1501at 1689.374 kb on - strand, within Psyr_1501at 1689.374 kb on - strand, within Psyr_1501at 1689.374 kb on - strand, within Psyr_1501at 1689.383 kb on - strand, within Psyr_1501at 1689.383 kb on - strand, within Psyr_1501at 1689.610 kb on + strandat 1689.610 kb on + strandat 1689.616 kb on + strandat 1689.616 kb on + strandat 1689.616 kb on + strandat 1689.616 kb on + strandat 1689.616 kb on + strandat 1689.617 kb on - strandat 1689.678 kb on + strandat 1689.678 kb on + strandat 1689.678 kb on + strandat 1689.678 kb on + strandat 1689.678 kb on + strandat 1689.678 kb on + strandat 1689.679 kb on - strandat 1689.679 kb on - strandat 1689.679 kb on - strandat 1689.679 kb on - strandat 1689.679 kb on - strandat 1689.679 kb on - strandat 1689.679 kb on - strandat 1689.679 kb on - strandat 1689.679 kb on - strandat 1689.761 kb on + strand, within Psyr_1502at 1689.865 kb on + strand, within Psyr_1502at 1689.865 kb on + strand, within Psyr_1502at 1689.865 kb on + strand, within Psyr_1502at 1689.866 kb on - strand, within Psyr_1502at 1689.866 kb on - strand, within Psyr_1502at 1689.866 kb on - strand, within Psyr_1502at 1689.866 kb on - strand, within Psyr_1502at 1689.866 kb on - strand, within Psyr_1502at 1689.868 kb on - strand, within Psyr_1502

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Per-strain Table

Position Strand Gene LocusTag Fraction KB with Gambogic Acid 10 mM
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1,687,365 + Psyr_1498 0.70 +0.2
1,687,366 - Psyr_1498 0.70 +0.3
1,687,368 + Psyr_1498 0.70 +0.7
1,687,369 - Psyr_1498 0.70 +0.3
1,687,369 - Psyr_1498 0.70 -0.2
1,687,369 - Psyr_1498 0.70 +0.5
1,687,402 + Psyr_1498 0.72 -0.3
1,687,402 + Psyr_1498 0.72 -0.3
1,687,403 - Psyr_1498 0.73 -0.1
1,687,403 - Psyr_1498 0.73 +2.0
1,687,403 - Psyr_1498 0.73 -1.5
1,687,453 + Psyr_1498 0.76 +0.3
1,687,454 + Psyr_1498 0.76 -1.6
1,687,455 - Psyr_1498 0.76 -1.1
1,687,490 + Psyr_1498 0.79 +0.4
1,687,490 + Psyr_1498 0.79 +1.8
1,687,490 + Psyr_1498 0.79 -1.7
1,687,544 - Psyr_1498 0.83 +1.3
1,687,576 + Psyr_1498 0.85 -1.3
1,687,576 + Psyr_1498 0.85 +0.3
1,687,704 - +0.6
1,687,706 + -0.5
1,687,706 + +1.3
1,687,707 - +0.9
1,687,707 - -2.7
1,687,707 - -0.4
1,687,715 + -1.0
1,687,715 + +0.1
1,687,715 + +1.3
1,687,715 + +0.6
1,687,715 + +0.5
1,687,715 + -0.2
1,687,715 + +0.2
1,687,715 + +0.9
1,687,715 + -1.6
1,687,715 + +0.7
1,687,716 - -0.3
1,687,716 - +0.4
1,688,055 + Psyr_1499 0.44 -1.3
1,688,055 + Psyr_1499 0.44 +1.3
1,688,056 - Psyr_1499 0.44 -0.7
1,688,056 - Psyr_1499 0.44 +1.1
1,688,056 - Psyr_1499 0.44 -0.2
1,688,164 + Psyr_1499 0.68 +0.2
1,688,164 + Psyr_1499 0.68 +0.5
1,688,294 + -2.3
1,688,295 - +0.3
1,688,295 - +0.5
1,688,295 - -2.3
1,688,301 + -0.9
1,688,301 + +1.5
1,688,301 + -0.0
1,688,301 + +0.3
1,688,301 + +1.3
1,688,302 - +0.6
1,688,302 - -1.8
1,688,302 - -1.0
1,688,304 - +1.9
1,688,326 - -0.8
1,688,327 + -0.9
1,688,327 + -0.3
1,688,349 + +0.3
1,688,349 + +1.7
1,688,349 + +0.4
1,688,350 - -1.0
1,688,350 - +0.1
1,688,350 - -1.3
1,688,469 + Psyr_1500 0.22 +0.1
1,688,469 + Psyr_1500 0.22 +0.4
1,688,469 + Psyr_1500 0.22 +0.7
1,688,469 + Psyr_1500 0.22 -0.0
1,688,469 + Psyr_1500 0.22 -0.6
1,688,469 + Psyr_1500 0.22 +1.2
1,688,469 + Psyr_1500 0.22 +0.8
1,688,470 - Psyr_1500 0.22 +0.9
1,688,470 - Psyr_1500 0.22 -1.2
1,688,470 - Psyr_1500 0.22 -0.9
1,688,470 - Psyr_1500 0.22 +0.3
1,688,470 - Psyr_1500 0.22 -0.6
1,688,470 - Psyr_1500 0.22 -1.1
1,688,470 - Psyr_1500 0.22 -0.2
1,688,470 - Psyr_1500 0.22 +0.7
1,688,492 + Psyr_1500 0.26 +0.7
1,688,505 - Psyr_1500 0.28 -0.9
1,688,505 - Psyr_1500 0.28 +2.2
1,688,505 - Psyr_1500 0.28 -1.2
1,688,510 + Psyr_1500 0.29 +1.4
1,688,593 - Psyr_1500 0.44 +0.7
1,688,593 - Psyr_1500 0.44 -0.3
1,688,637 + Psyr_1500 0.52 -0.2
1,688,637 + Psyr_1500 0.52 -0.1
1,688,637 + Psyr_1500 0.52 +3.1
1,688,637 + Psyr_1500 0.52 +1.1
1,688,637 + Psyr_1500 0.52 +1.1
1,688,637 + -0.7
1,688,637 + Psyr_1500 0.52 -0.7
1,688,637 + Psyr_1500 0.52 +1.3
1,688,638 - Psyr_1500 0.52 -1.3
1,688,638 - Psyr_1500 0.52 +1.0
1,688,638 - Psyr_1500 0.52 -0.0
1,688,638 - Psyr_1500 0.52 +1.6
1,688,638 - Psyr_1500 0.52 -1.1
1,688,638 - Psyr_1500 0.52 -1.6
1,688,638 - Psyr_1500 0.52 +1.6
1,688,638 - Psyr_1500 0.52 -0.0
1,688,638 - Psyr_1500 0.52 -0.2
1,688,638 - Psyr_1500 0.52 -0.1
1,688,664 + Psyr_1500 0.57 +0.2
1,688,664 + Psyr_1500 0.57 -0.2
1,688,700 + Psyr_1500 0.63 +1.3
1,688,700 + Psyr_1500 0.63 -0.2
1,688,742 + Psyr_1500 0.71 +0.6
1,688,838 + Psyr_1500 0.88 +0.6
1,688,838 + Psyr_1500 0.88 -1.3
1,688,838 + Psyr_1500 0.88 -0.3
1,688,838 + Psyr_1500 0.88 +0.7
1,688,838 + Psyr_1500 0.88 +1.4
1,688,838 + Psyr_1500 0.88 -2.6
1,688,838 + Psyr_1500 0.88 +0.3
1,688,838 + Psyr_1500 0.88 +1.1
1,688,838 + Psyr_1500 0.88 +1.8
1,688,838 + Psyr_1500 0.88 -0.4
1,688,838 + Psyr_1500 0.88 +1.2
1,688,838 + Psyr_1500 0.88 -0.9
1,688,838 + Psyr_1500 0.88 -0.8
1,688,839 - Psyr_1500 0.88 +0.5
1,688,839 - Psyr_1500 0.88 +0.5
1,688,839 - Psyr_1500 0.88 -0.6
1,688,839 - Psyr_1500 0.88 -1.3
1,688,839 - Psyr_1500 0.88 -0.6
1,688,839 - Psyr_1500 0.88 +0.3
1,688,839 - Psyr_1500 0.88 +1.5
1,688,839 - Psyr_1500 0.88 +0.3
1,688,839 - Psyr_1500 0.88 +0.8
1,688,839 - Psyr_1500 0.88 -1.3
1,688,839 - Psyr_1500 0.88 +0.2
1,688,839 - Psyr_1500 0.88 +1.0
1,688,839 - Psyr_1500 0.88 +0.4
1,688,842 + Psyr_1500 0.89 +1.6
1,688,842 + Psyr_1500 0.89 +0.4
1,688,921 + -3.0
1,688,983 - +1.1
1,689,002 - +0.7
1,689,008 + Psyr_1501 0.10 -0.8
1,689,008 + Psyr_1501 0.10 +0.3
1,689,009 - Psyr_1501 0.10 -0.1
1,689,009 - Psyr_1501 0.10 -1.3
1,689,009 - Psyr_1501 0.10 +0.4
1,689,077 + Psyr_1501 0.20 -1.9
1,689,078 - Psyr_1501 0.20 +1.4
1,689,078 - Psyr_1501 0.20 +0.7
1,689,078 - Psyr_1501 0.20 -0.2
1,689,078 - Psyr_1501 0.20 +0.8
1,689,080 + Psyr_1501 0.20 +0.2
1,689,080 + Psyr_1501 0.20 -0.3
1,689,080 + Psyr_1501 0.20 -0.0
1,689,227 - Psyr_1501 0.40 +0.5
1,689,266 - Psyr_1501 0.45 +0.0
1,689,374 - Psyr_1501 0.60 +1.1
1,689,374 - Psyr_1501 0.60 +0.9
1,689,374 - Psyr_1501 0.60 -0.0
1,689,374 - Psyr_1501 0.60 +0.2
1,689,383 - Psyr_1501 0.61 +0.0
1,689,383 - Psyr_1501 0.61 +1.1
1,689,610 + -2.7
1,689,610 + -2.4
1,689,616 + +0.1
1,689,616 + -0.3
1,689,616 + -0.1
1,689,616 + +0.4
1,689,616 + +1.1
1,689,617 - -0.3
1,689,678 + +0.4
1,689,678 + -1.1
1,689,678 + +1.3
1,689,678 + +0.1
1,689,678 + -0.3
1,689,678 + -0.5
1,689,679 - +0.1
1,689,679 - -0.9
1,689,679 - -0.3
1,689,679 - -2.3
1,689,679 - +0.2
1,689,679 - -0.5
1,689,679 - +0.1
1,689,679 - -1.9
1,689,679 - +2.3
1,689,761 + Psyr_1502 0.20 -0.0
1,689,865 + Psyr_1502 0.42 +0.0
1,689,865 + Psyr_1502 0.42 -0.3
1,689,865 + Psyr_1502 0.42 -0.1
1,689,866 - Psyr_1502 0.42 -1.1
1,689,866 - Psyr_1502 0.42 -0.2
1,689,866 - Psyr_1502 0.42 -0.8
1,689,866 - Psyr_1502 0.42 -0.7
1,689,866 - Psyr_1502 0.42 +1.0
1,689,868 - Psyr_1502 0.43 +2.7

Or see this region's nucleotide sequence