Experiment: KB with Gambogic Acid 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0055 and Psyr_0056 are separated by 77 nucleotides Psyr_0056 and Psyr_0057 overlap by 10 nucleotides Psyr_0057 and Psyr_0058 are separated by 4 nucleotides Psyr_0058 and Psyr_0059 are separated by 216 nucleotides
Psyr_0055: Psyr_0055 - FKBP-type peptidyl-prolyl isomerase, N-terminal:Peptidylprolyl isomerase, FKBP-type, at 56,138 to 56,815
_0055
Psyr_0056: Psyr_0056 - Regulator of RNA polymerase sigma(70) subunit, Rsd/AlgQ, at 56,893 to 57,393
_0056
Psyr_0057: Psyr_0057 - conserved hypothetical protein, at 57,384 to 57,536
_0057
Psyr_0058: Psyr_0058 - Disulfide bond formation protein DsbB, at 57,541 to 58,068
_0058
Psyr_0059: Psyr_0059 - HemY, N-terminal:HemY, N-terminal:HemY, N-terminal, at 58,285 to 59,529
_0059
Position (kb) 57
58
59 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 56.579 kb on + strand, within Psyr_0055 at 56.580 kb on - strand, within Psyr_0055 at 56.580 kb on - strand, within Psyr_0055 at 56.580 kb on - strand, within Psyr_0055 at 56.732 kb on + strand, within Psyr_0055 at 56.732 kb on + strand, within Psyr_0055 at 56.732 kb on + strand, within Psyr_0055 at 56.733 kb on - strand, within Psyr_0055 at 56.771 kb on - strand at 56.782 kb on - strand at 56.782 kb on - strand at 56.813 kb on + strand at 56.813 kb on + strand at 56.813 kb on + strand at 56.814 kb on - strand at 56.814 kb on - strand at 56.814 kb on - strand at 56.856 kb on + strand at 56.856 kb on + strand at 56.856 kb on + strand at 56.856 kb on + strand at 56.857 kb on - strand at 56.857 kb on - strand at 56.857 kb on - strand at 56.857 kb on - strand at 56.873 kb on + strand at 56.873 kb on + strand at 56.873 kb on + strand at 56.873 kb on + strand at 56.874 kb on - strand at 56.874 kb on - strand at 56.874 kb on - strand at 56.928 kb on + strand at 56.928 kb on + strand at 56.929 kb on - strand at 57.092 kb on + strand, within Psyr_0056 at 57.161 kb on + strand, within Psyr_0056 at 57.161 kb on + strand, within Psyr_0056 at 57.161 kb on + strand, within Psyr_0056 at 57.188 kb on + strand, within Psyr_0056 at 57.188 kb on + strand, within Psyr_0056 at 57.188 kb on + strand, within Psyr_0056 at 57.188 kb on + strand, within Psyr_0056 at 57.188 kb on + strand, within Psyr_0056 at 57.188 kb on + strand, within Psyr_0056 at 57.188 kb on + strand, within Psyr_0056 at 57.189 kb on - strand, within Psyr_0056 at 57.189 kb on - strand, within Psyr_0056 at 57.189 kb on - strand, within Psyr_0056 at 57.189 kb on - strand, within Psyr_0056 at 57.189 kb on - strand, within Psyr_0056 at 57.269 kb on + strand, within Psyr_0056 at 57.269 kb on + strand, within Psyr_0056 at 57.270 kb on - strand, within Psyr_0056 at 57.270 kb on - strand, within Psyr_0056 at 57.416 kb on - strand, within Psyr_0057 at 57.416 kb on - strand, within Psyr_0057 at 57.416 kb on - strand, within Psyr_0057 at 57.416 kb on - strand, within Psyr_0057 at 57.416 kb on - strand, within Psyr_0057 at 57.438 kb on + strand, within Psyr_0057 at 57.439 kb on - strand, within Psyr_0057 at 57.439 kb on - strand, within Psyr_0057 at 57.439 kb on - strand, within Psyr_0057 at 57.439 kb on - strand, within Psyr_0057 at 57.457 kb on + strand, within Psyr_0057 at 57.457 kb on + strand, within Psyr_0057 at 57.457 kb on + strand, within Psyr_0057 at 57.457 kb on + strand, within Psyr_0057 at 57.457 kb on + strand, within Psyr_0057 at 57.457 kb on + strand, within Psyr_0057 at 57.457 kb on + strand, within Psyr_0057 at 57.457 kb on + strand, within Psyr_0057 at 57.457 kb on + strand, within Psyr_0057 at 57.458 kb on - strand, within Psyr_0057 at 57.458 kb on - strand, within Psyr_0057 at 57.458 kb on - strand, within Psyr_0057 at 57.458 kb on - strand, within Psyr_0057 at 57.485 kb on + strand, within Psyr_0057 at 57.485 kb on + strand, within Psyr_0057 at 57.512 kb on + strand, within Psyr_0057 at 57.513 kb on - strand, within Psyr_0057 at 57.513 kb on - strand, within Psyr_0057 at 57.523 kb on + strand at 57.523 kb on + strand at 57.523 kb on + strand at 57.532 kb on + strand at 57.532 kb on + strand at 57.533 kb on - strand at 57.533 kb on - strand at 57.533 kb on - strand at 57.533 kb on - strand at 57.533 kb on - strand at 57.533 kb on - strand at 57.624 kb on + strand, within Psyr_0058 at 57.624 kb on + strand, within Psyr_0058 at 57.625 kb on - strand, within Psyr_0058 at 57.625 kb on - strand, within Psyr_0058 at 57.625 kb on - strand, within Psyr_0058 at 57.625 kb on - strand, within Psyr_0058 at 57.625 kb on - strand, within Psyr_0058 at 57.625 kb on - strand, within Psyr_0058 at 57.625 kb on - strand, within Psyr_0058 at 57.625 kb on - strand, within Psyr_0058 at 57.658 kb on - strand, within Psyr_0058 at 57.723 kb on + strand, within Psyr_0058 at 57.723 kb on + strand, within Psyr_0058 at 57.723 kb on + strand, within Psyr_0058 at 57.724 kb on - strand, within Psyr_0058 at 57.724 kb on - strand, within Psyr_0058 at 57.724 kb on - strand, within Psyr_0058 at 57.746 kb on - strand, within Psyr_0058 at 57.746 kb on - strand, within Psyr_0058 at 57.976 kb on + strand, within Psyr_0058 at 57.977 kb on - strand, within Psyr_0058 at 57.992 kb on + strand, within Psyr_0058 at 57.992 kb on + strand, within Psyr_0058 at 57.993 kb on - strand, within Psyr_0058 at 57.993 kb on - strand, within Psyr_0058 at 57.993 kb on - strand, within Psyr_0058 at 58.064 kb on + strand at 58.064 kb on + strand at 58.064 kb on + strand at 58.064 kb on + strand at 58.064 kb on + strand at 58.064 kb on + strand at 58.064 kb on + strand at 58.064 kb on + strand at 58.064 kb on + strand at 58.064 kb on + strand at 58.064 kb on + strand at 58.065 kb on - strand at 58.065 kb on - strand at 58.065 kb on - strand at 58.065 kb on - strand at 58.065 kb on - strand at 58.065 kb on - strand at 58.065 kb on - strand at 58.065 kb on - strand at 58.065 kb on - strand at 58.065 kb on - strand at 58.065 kb on - strand at 58.065 kb on - strand at 58.065 kb on - strand at 58.065 kb on - strand at 58.065 kb on - strand at 58.065 kb on - strand at 58.065 kb on - strand at 58.065 kb on - strand at 58.099 kb on - strand at 58.194 kb on + strand at 58.194 kb on + strand at 58.194 kb on + strand at 58.194 kb on + strand at 58.195 kb on - strand at 58.195 kb on - strand at 58.195 kb on - strand at 58.206 kb on + strand at 58.206 kb on + strand at 58.206 kb on + strand at 58.206 kb on + strand at 58.497 kb on + strand, within Psyr_0059 at 58.497 kb on + strand, within Psyr_0059 at 58.497 kb on + strand, within Psyr_0059 at 58.497 kb on + strand, within Psyr_0059 at 58.497 kb on + strand, within Psyr_0059 at 58.497 kb on + strand, within Psyr_0059 at 58.497 kb on + strand, within Psyr_0059 at 58.497 kb on + strand, within Psyr_0059 at 58.497 kb on + strand, within Psyr_0059 at 58.498 kb on - strand, within Psyr_0059 at 58.498 kb on - strand, within Psyr_0059 at 58.498 kb on - strand, within Psyr_0059 at 58.498 kb on - strand, within Psyr_0059 at 58.498 kb on - strand, within Psyr_0059 at 58.498 kb on - strand, within Psyr_0059 at 58.498 kb on - strand, within Psyr_0059 at 58.498 kb on - strand, within Psyr_0059 at 58.498 kb on - strand, within Psyr_0059 at 58.498 kb on - strand, within Psyr_0059 at 58.498 kb on - strand, within Psyr_0059 at 58.498 kb on - strand, within Psyr_0059 at 58.498 kb on - strand, within Psyr_0059 at 58.601 kb on + strand, within Psyr_0059 at 58.625 kb on + strand, within Psyr_0059 at 58.625 kb on + strand, within Psyr_0059 at 58.625 kb on + strand, within Psyr_0059 at 58.625 kb on + strand, within Psyr_0059 at 58.625 kb on + strand, within Psyr_0059 at 58.700 kb on + strand, within Psyr_0059 at 58.938 kb on - strand, within Psyr_0059 at 58.938 kb on - strand, within Psyr_0059
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Gambogic Acid 10 mM remove 56,579 + Psyr_0055 0.65 -0.4 56,580 - Psyr_0055 0.65 -0.0 56,580 - Psyr_0055 0.65 -2.8 56,580 - Psyr_0055 0.65 -0.6 56,732 + Psyr_0055 0.88 +1.1 56,732 + Psyr_0055 0.88 -1.3 56,732 + Psyr_0055 0.88 +0.4 56,733 - Psyr_0055 0.88 +0.8 56,771 - -2.2 56,782 - +0.4 56,782 - -0.0 56,813 + -0.4 56,813 + +0.5 56,813 + -1.6 56,814 - +1.4 56,814 - -0.6 56,814 - +0.4 56,856 + +0.4 56,856 + +0.2 56,856 + -1.1 56,856 + -0.3 56,857 - +0.7 56,857 - +0.3 56,857 - -0.0 56,857 - -0.1 56,873 + -0.3 56,873 + +0.4 56,873 + -0.5 56,873 + -0.7 56,874 - +0.3 56,874 - -1.3 56,874 - +1.4 56,928 + -0.0 56,928 + +0.7 56,929 - -0.5 57,092 + Psyr_0056 0.40 +0.5 57,161 + Psyr_0056 0.53 +0.2 57,161 + Psyr_0056 0.53 +0.4 57,161 + Psyr_0056 0.53 -0.2 57,188 + Psyr_0056 0.59 -2.4 57,188 + Psyr_0056 0.59 -0.4 57,188 + Psyr_0056 0.59 +0.8 57,188 + Psyr_0056 0.59 +0.8 57,188 + Psyr_0056 0.59 -0.1 57,188 + Psyr_0056 0.59 +0.4 57,188 + Psyr_0056 0.59 +0.5 57,189 - Psyr_0056 0.59 -1.2 57,189 - Psyr_0056 0.59 -0.4 57,189 - Psyr_0056 0.59 -0.4 57,189 - Psyr_0056 0.59 -1.0 57,189 - Psyr_0056 0.59 +0.0 57,269 + Psyr_0056 0.75 -0.7 57,269 + Psyr_0056 0.75 -0.4 57,270 - Psyr_0056 0.75 -0.6 57,270 - Psyr_0056 0.75 +1.9 57,416 - Psyr_0057 0.21 +1.4 57,416 - Psyr_0057 0.21 +2.7 57,416 - Psyr_0057 0.21 -0.9 57,416 - Psyr_0057 0.21 -1.9 57,416 - Psyr_0057 0.21 +0.5 57,438 + Psyr_0057 0.35 +0.2 57,439 - Psyr_0057 0.36 -0.6 57,439 - Psyr_0057 0.36 +0.8 57,439 - Psyr_0057 0.36 -0.3 57,439 - Psyr_0057 0.36 -1.0 57,457 + Psyr_0057 0.48 +1.2 57,457 + Psyr_0057 0.48 -0.1 57,457 + Psyr_0057 0.48 +0.2 57,457 + Psyr_0057 0.48 -0.2 57,457 + Psyr_0057 0.48 +1.3 57,457 + Psyr_0057 0.48 -0.2 57,457 + Psyr_0057 0.48 -0.5 57,457 + Psyr_0057 0.48 +0.8 57,457 + Psyr_0057 0.48 -1.6 57,458 - Psyr_0057 0.48 +1.4 57,458 - Psyr_0057 0.48 -0.0 57,458 - Psyr_0057 0.48 -0.6 57,458 - Psyr_0057 0.48 -1.6 57,485 + Psyr_0057 0.66 -0.9 57,485 + Psyr_0057 0.66 -0.1 57,512 + Psyr_0057 0.84 +0.3 57,513 - Psyr_0057 0.84 -0.4 57,513 - Psyr_0057 0.84 +0.5 57,523 + -0.2 57,523 + +0.7 57,523 + +0.4 57,532 + -0.9 57,532 + +0.4 57,533 - +0.5 57,533 - +1.4 57,533 - -0.5 57,533 - -0.2 57,533 - -0.1 57,533 - +0.2 57,624 + Psyr_0058 0.16 -0.9 57,624 + Psyr_0058 0.16 -0.2 57,625 - Psyr_0058 0.16 -1.6 57,625 - Psyr_0058 0.16 -0.2 57,625 - Psyr_0058 0.16 +0.7 57,625 - Psyr_0058 0.16 -0.7 57,625 - Psyr_0058 0.16 +0.4 57,625 - Psyr_0058 0.16 +0.2 57,625 - Psyr_0058 0.16 -0.6 57,625 - Psyr_0058 0.16 +0.5 57,658 - Psyr_0058 0.22 +0.1 57,723 + Psyr_0058 0.34 +0.4 57,723 + Psyr_0058 0.34 +0.3 57,723 + Psyr_0058 0.34 -0.6 57,724 - Psyr_0058 0.35 -0.6 57,724 - Psyr_0058 0.35 -1.5 57,724 - Psyr_0058 0.35 +0.6 57,746 - Psyr_0058 0.39 +1.0 57,746 - Psyr_0058 0.39 +0.7 57,976 + Psyr_0058 0.82 -0.9 57,977 - Psyr_0058 0.83 +0.2 57,992 + Psyr_0058 0.85 -0.4 57,992 + Psyr_0058 0.85 -2.3 57,993 - Psyr_0058 0.86 -0.3 57,993 - Psyr_0058 0.86 -0.3 57,993 - Psyr_0058 0.86 -0.5 58,064 + +0.6 58,064 + +0.4 58,064 + +2.2 58,064 + +0.2 58,064 + +0.0 58,064 + +0.4 58,064 + +0.7 58,064 + -0.3 58,064 + -0.2 58,064 + +1.0 58,064 + -0.0 58,065 - +0.2 58,065 - -1.0 58,065 - -0.1 58,065 - +0.6 58,065 - -2.7 58,065 - +1.6 58,065 - +1.2 58,065 - -1.4 58,065 - +0.7 58,065 - -0.1 58,065 - +0.9 58,065 - +0.2 58,065 - -0.6 58,065 - -0.4 58,065 - +0.9 58,065 - +1.7 58,065 - +0.1 58,065 - +0.8 58,099 - +1.1 58,194 + -1.1 58,194 + +0.9 58,194 + +0.1 58,194 + -0.3 58,195 - +1.4 58,195 - +0.3 58,195 - +0.8 58,206 + +0.7 58,206 + -0.9 58,206 + -0.6 58,206 + +0.3 58,497 + Psyr_0059 0.17 -2.5 58,497 + Psyr_0059 0.17 -0.4 58,497 + Psyr_0059 0.17 -1.5 58,497 + Psyr_0059 0.17 -0.7 58,497 + Psyr_0059 0.17 +1.4 58,497 + Psyr_0059 0.17 +0.3 58,497 + Psyr_0059 0.17 -0.6 58,497 + Psyr_0059 0.17 -0.6 58,497 + Psyr_0059 0.17 -1.0 58,498 - Psyr_0059 0.17 +0.2 58,498 - Psyr_0059 0.17 +0.9 58,498 - Psyr_0059 0.17 +2.0 58,498 - Psyr_0059 0.17 +0.4 58,498 - Psyr_0059 0.17 -1.1 58,498 - Psyr_0059 0.17 -1.2 58,498 - Psyr_0059 0.17 -0.5 58,498 - Psyr_0059 0.17 +0.5 58,498 - Psyr_0059 0.17 +0.3 58,498 - Psyr_0059 0.17 +0.3 58,498 - Psyr_0059 0.17 +0.5 58,498 - Psyr_0059 0.17 +0.7 58,498 - Psyr_0059 0.17 -0.6 58,601 + Psyr_0059 0.25 +1.0 58,625 + Psyr_0059 0.27 -0.1 58,625 + Psyr_0059 0.27 -1.0 58,625 + Psyr_0059 0.27 -0.6 58,625 + Psyr_0059 0.27 +0.9 58,625 + Psyr_0059 0.27 -2.4 58,700 + Psyr_0059 0.33 -1.4 58,938 - Psyr_0059 0.52 -0.4 58,938 - Psyr_0059 0.52 -1.0
Or see this region's nucleotide sequence