Experiment: Ferric citrate electron acceptor
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hypE and hypD overlap by 8 nucleotides hypD and hypC are separated by 62 nucleotides hypC and hypB overlap by 1 nucleotides
SO2090: hypE - hydrogenase expression/formation protein HypE (NCBI ptt file), at 2,186,020 to 2,187,039
hypE
SO2091: hypD - hydrogenase expression/formation protein HypD (NCBI ptt file), at 2,187,032 to 2,188,171
hypD
SO2092: hypC - hydrogenase assembly chaperone hypC/hupF (NCBI ptt file), at 2,188,234 to 2,188,479
hypC
SO2093: hypB - hydrogenase accessory protein HypB (NCBI ptt file), at 2,188,479 to 2,189,273
hypB
Position (kb)
2187
2188
2189 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2186.151 kb on + strand, within hypE at 2186.267 kb on + strand, within hypE at 2186.274 kb on - strand, within hypE at 2186.454 kb on + strand, within hypE at 2186.631 kb on - strand, within hypE at 2186.788 kb on + strand, within hypE at 2186.788 kb on + strand, within hypE at 2186.863 kb on - strand, within hypE at 2186.869 kb on + strand, within hypE at 2186.920 kb on + strand, within hypE at 2187.035 kb on + strand at 2187.094 kb on + strand at 2187.094 kb on + strand at 2187.154 kb on + strand, within hypD at 2187.207 kb on + strand, within hypD at 2187.207 kb on + strand, within hypD at 2187.313 kb on + strand, within hypD at 2187.313 kb on + strand, within hypD at 2187.313 kb on + strand at 2187.313 kb on + strand, within hypD at 2187.537 kb on - strand, within hypD at 2187.557 kb on + strand, within hypD at 2187.566 kb on + strand, within hypD at 2187.595 kb on - strand, within hypD at 2187.718 kb on + strand, within hypD at 2187.780 kb on + strand, within hypD at 2187.880 kb on + strand, within hypD at 2187.880 kb on + strand, within hypD at 2187.903 kb on + strand, within hypD at 2187.949 kb on + strand, within hypD at 2188.070 kb on - strand at 2188.075 kb on + strand at 2188.289 kb on - strand, within hypC at 2188.404 kb on + strand, within hypC at 2188.404 kb on + strand, within hypC at 2188.404 kb on + strand, within hypC at 2188.509 kb on - strand at 2188.853 kb on + strand, within hypB at 2188.861 kb on - strand, within hypB at 2188.861 kb on - strand, within hypB at 2188.954 kb on + strand, within hypB at 2188.975 kb on + strand, within hypB at 2188.996 kb on + strand, within hypB at 2189.004 kb on - strand, within hypB at 2189.060 kb on - strand, within hypB at 2189.079 kb on + strand, within hypB at 2189.079 kb on + strand, within hypB at 2189.158 kb on - strand, within hypB
Per-strain Table
Position Strand Gene LocusTag Fraction Ferric citrate electron acceptor remove 2,186,151 + hypE SO2090 0.13 -0.0 2,186,267 + hypE SO2090 0.24 +0.1 2,186,274 - hypE SO2090 0.25 -0.1 2,186,454 + hypE SO2090 0.43 -0.0 2,186,631 - hypE SO2090 0.60 +0.8 2,186,788 + hypE SO2090 0.75 +0.3 2,186,788 + hypE SO2090 0.75 -0.7 2,186,863 - hypE SO2090 0.83 +1.0 2,186,869 + hypE SO2090 0.83 +0.7 2,186,920 + hypE SO2090 0.88 +0.3 2,187,035 + +2.4 2,187,094 + -0.2 2,187,094 + -0.0 2,187,154 + hypD SO2091 0.11 -0.1 2,187,207 + hypD SO2091 0.15 +0.2 2,187,207 + hypD SO2091 0.15 +0.7 2,187,313 + hypD SO2091 0.25 +0.4 2,187,313 + hypD SO2091 0.25 +1.1 2,187,313 + -0.2 2,187,313 + hypD SO2091 0.25 +1.5 2,187,537 - hypD SO2091 0.44 +1.1 2,187,557 + hypD SO2091 0.46 +2.5 2,187,566 + hypD SO2091 0.47 -0.4 2,187,595 - hypD SO2091 0.49 -1.5 2,187,718 + hypD SO2091 0.60 +0.7 2,187,780 + hypD SO2091 0.66 +1.4 2,187,880 + hypD SO2091 0.74 -1.4 2,187,880 + hypD SO2091 0.74 -0.0 2,187,903 + hypD SO2091 0.76 +0.4 2,187,949 + hypD SO2091 0.80 -1.2 2,188,070 - -2.0 2,188,075 + +0.7 2,188,289 - hypC SO2092 0.22 +0.2 2,188,404 + hypC SO2092 0.69 +1.2 2,188,404 + hypC SO2092 0.69 +0.6 2,188,404 + hypC SO2092 0.69 +0.8 2,188,509 - -1.8 2,188,853 + hypB SO2093 0.47 +0.5 2,188,861 - hypB SO2093 0.48 +0.6 2,188,861 - hypB SO2093 0.48 -0.2 2,188,954 + hypB SO2093 0.60 -0.5 2,188,975 + hypB SO2093 0.62 -0.3 2,188,996 + hypB SO2093 0.65 -0.2 2,189,004 - hypB SO2093 0.66 +1.8 2,189,060 - hypB SO2093 0.73 +0.1 2,189,079 + hypB SO2093 0.75 -1.4 2,189,079 + hypB SO2093 0.75 -1.4 2,189,158 - hypB SO2093 0.85 +1.8
Or see this region's nucleotide sequence