Experiment: Ferric citrate electron acceptor
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tyrP and hisI are separated by 579 nucleotides hisI and hisF are separated by 82 nucleotides hisF and hisA overlap by 19 nucleotides
SO2065: tyrP - tyrosine-specific transport protein (NCBI ptt file), at 2,162,328 to 2,163,515
tyrP
SO2067: hisI - phosphoribosyl-ATP pyrophosphatase/phosphoribosyl-AMP cyclohydrolase (NCBI ptt file), at 2,164,095 to 2,164,730
hisI
SO2068: hisF - hisF protein (cyclase) (NCBI ptt file), at 2,164,813 to 2,165,586
hisF
SO2069: hisA - phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase (NCBI ptt file), at 2,165,568 to 2,166,305
hisA
Position (kb)
2164
2165 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2163.101 kb on - strand, within tyrP at 2163.101 kb on - strand, within tyrP at 2163.354 kb on + strand, within tyrP at 2163.359 kb on + strand, within tyrP at 2163.424 kb on - strand at 2163.424 kb on - strand at 2163.458 kb on + strand at 2163.630 kb on + strand at 2163.657 kb on + strand at 2163.703 kb on - strand at 2163.876 kb on - strand at 2164.152 kb on + strand at 2164.153 kb on - strand at 2164.207 kb on + strand, within hisI at 2164.318 kb on + strand, within hisI at 2164.511 kb on + strand, within hisI at 2164.600 kb on + strand, within hisI at 2164.800 kb on + strand at 2164.800 kb on + strand at 2164.851 kb on + strand at 2164.917 kb on - strand, within hisF at 2164.924 kb on + strand, within hisF at 2164.953 kb on + strand, within hisF at 2165.133 kb on - strand, within hisF at 2165.306 kb on - strand, within hisF at 2165.328 kb on - strand, within hisF at 2165.390 kb on + strand, within hisF at 2165.398 kb on - strand, within hisF at 2165.421 kb on - strand, within hisF at 2165.528 kb on + strand at 2165.529 kb on - strand at 2165.561 kb on - strand at 2165.583 kb on + strand at 2165.602 kb on + strand at 2165.660 kb on + strand, within hisA at 2165.695 kb on - strand, within hisA
Per-strain Table
Position Strand Gene LocusTag Fraction Ferric citrate electron acceptor remove 2,163,101 - tyrP SO2065 0.65 -1.5 2,163,101 - tyrP SO2065 0.65 +2.8 2,163,354 + tyrP SO2065 0.86 +0.1 2,163,359 + tyrP SO2065 0.87 +1.1 2,163,424 - -0.2 2,163,424 - -0.3 2,163,458 + +1.2 2,163,630 + +0.4 2,163,657 + +0.6 2,163,703 - +4.6 2,163,876 - +1.5 2,164,152 + -2.0 2,164,153 - -0.6 2,164,207 + hisI SO2067 0.18 -3.0 2,164,318 + hisI SO2067 0.35 -1.6 2,164,511 + hisI SO2067 0.65 -1.8 2,164,600 + hisI SO2067 0.79 -1.2 2,164,800 + +0.9 2,164,800 + -0.4 2,164,851 + +1.5 2,164,917 - hisF SO2068 0.13 +0.2 2,164,924 + hisF SO2068 0.14 +0.4 2,164,953 + hisF SO2068 0.18 +1.5 2,165,133 - hisF SO2068 0.41 -1.4 2,165,306 - hisF SO2068 0.64 +0.0 2,165,328 - hisF SO2068 0.67 -0.4 2,165,390 + hisF SO2068 0.75 -0.8 2,165,398 - hisF SO2068 0.76 -1.7 2,165,421 - hisF SO2068 0.79 -1.0 2,165,528 + +0.1 2,165,529 - -0.8 2,165,561 - -1.0 2,165,583 + +0.2 2,165,602 + +1.8 2,165,660 + hisA SO2069 0.12 +0.3 2,165,695 - hisA SO2069 0.17 -0.8
Or see this region's nucleotide sequence