Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS20400

Experiment: RCH2_defined_Glucose_noNitrogen in EcoFAB_3.5mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS20395 and BWI76_RS20400 are separated by 40 nucleotidesBWI76_RS20400 and BWI76_RS20405 are separated by 206 nucleotides BWI76_RS20395: BWI76_RS20395 - flagella biosynthesis regulator, at 4,269,029 to 4,269,988 _RS20395 BWI76_RS20400: BWI76_RS20400 - arabinose transporter, at 4,270,029 to 4,271,204 _RS20400 BWI76_RS20405: BWI76_RS20405 - beta-ketoacyl-[acyl-carrier-protein] synthase I, at 4,271,411 to 4,272,628 _RS20405 Position (kb) 4270 4271 4272Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 4269.086 kb on - strandat 4269.090 kb on + strandat 4269.093 kb on - strandat 4269.145 kb on - strand, within BWI76_RS20395at 4269.174 kb on + strand, within BWI76_RS20395at 4269.236 kb on + strand, within BWI76_RS20395at 4269.419 kb on + strand, within BWI76_RS20395at 4269.516 kb on - strand, within BWI76_RS20395at 4269.722 kb on - strand, within BWI76_RS20395at 4270.344 kb on - strand, within BWI76_RS20400at 4270.372 kb on - strand, within BWI76_RS20400at 4270.442 kb on + strand, within BWI76_RS20400at 4270.450 kb on - strand, within BWI76_RS20400at 4270.935 kb on + strand, within BWI76_RS20400at 4271.116 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_Glucose_noNitrogen in EcoFAB_3.5mL
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4,269,086 - -0.5
4,269,090 + -1.9
4,269,093 - -2.6
4,269,145 - BWI76_RS20395 0.12 +0.7
4,269,174 + BWI76_RS20395 0.15 +0.4
4,269,236 + BWI76_RS20395 0.22 -0.7
4,269,419 + BWI76_RS20395 0.41 +0.4
4,269,516 - BWI76_RS20395 0.51 -0.1
4,269,722 - BWI76_RS20395 0.72 +1.5
4,270,344 - BWI76_RS20400 0.27 +0.3
4,270,372 - BWI76_RS20400 0.29 -1.4
4,270,442 + BWI76_RS20400 0.35 +4.0
4,270,450 - BWI76_RS20400 0.36 +0.4
4,270,935 + BWI76_RS20400 0.77 +0.4
4,271,116 + +0.0

Or see this region's nucleotide sequence