Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS05660

Experiment: RCH2_defined_Glucose_noNitrogen in EcoFAB_3.5mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS05650 and BWI76_RS05655 are separated by 583 nucleotidesBWI76_RS05655 and BWI76_RS05660 are separated by 10 nucleotidesBWI76_RS05660 and BWI76_RS05665 are separated by 17 nucleotides BWI76_RS05650: BWI76_RS05650 - mannonate dehydratase, at 1,186,395 to 1,187,579 _RS05650 BWI76_RS05655: BWI76_RS05655 - hypothetical protein, at 1,188,163 to 1,188,420 _RS05655 BWI76_RS05660: BWI76_RS05660 - N-acetyltransferase, at 1,188,431 to 1,188,982 _RS05660 BWI76_RS05665: BWI76_RS05665 - SAM-dependent methyltransferase, at 1,189,000 to 1,190,628 _RS05665 Position (kb) 1188 1189Strain fitness (log2 ratio) -2 -1 0 1 2at 1187.552 kb on + strandat 1187.560 kb on - strandat 1187.606 kb on + strandat 1187.669 kb on - strandat 1187.778 kb on - strandat 1187.824 kb on + strandat 1187.837 kb on + strandat 1187.837 kb on + strandat 1187.845 kb on - strandat 1187.845 kb on - strandat 1187.845 kb on - strandat 1187.845 kb on - strandat 1188.010 kb on + strandat 1188.204 kb on + strand, within BWI76_RS05655at 1188.368 kb on - strand, within BWI76_RS05655at 1188.377 kb on - strand, within BWI76_RS05655at 1188.494 kb on + strand, within BWI76_RS05660at 1188.494 kb on + strand, within BWI76_RS05660at 1188.637 kb on + strand, within BWI76_RS05660at 1188.680 kb on - strand, within BWI76_RS05660at 1188.680 kb on - strand, within BWI76_RS05660at 1188.725 kb on + strand, within BWI76_RS05660at 1188.741 kb on + strand, within BWI76_RS05660at 1188.741 kb on + strand, within BWI76_RS05660at 1188.751 kb on + strand, within BWI76_RS05660at 1188.785 kb on - strand, within BWI76_RS05660at 1188.816 kb on + strand, within BWI76_RS05660at 1188.824 kb on - strand, within BWI76_RS05660at 1188.844 kb on - strand, within BWI76_RS05660at 1188.853 kb on + strand, within BWI76_RS05660at 1188.955 kb on + strandat 1189.046 kb on + strandat 1189.360 kb on - strand, within BWI76_RS05665at 1189.504 kb on + strand, within BWI76_RS05665at 1189.512 kb on - strand, within BWI76_RS05665at 1189.585 kb on + strand, within BWI76_RS05665at 1189.619 kb on - strand, within BWI76_RS05665at 1189.647 kb on + strand, within BWI76_RS05665at 1189.754 kb on + strand, within BWI76_RS05665at 1189.968 kb on - strand, within BWI76_RS05665

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_Glucose_noNitrogen in EcoFAB_3.5mL
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1,187,552 + +0.5
1,187,560 - +0.6
1,187,606 + +1.4
1,187,669 - -0.9
1,187,778 - +0.7
1,187,824 + +1.4
1,187,837 + -0.4
1,187,837 + +1.6
1,187,845 - +0.4
1,187,845 - -0.1
1,187,845 - +0.3
1,187,845 - +0.9
1,188,010 + -0.2
1,188,204 + BWI76_RS05655 0.16 -1.0
1,188,368 - BWI76_RS05655 0.79 -0.8
1,188,377 - BWI76_RS05655 0.83 -0.7
1,188,494 + BWI76_RS05660 0.11 -0.4
1,188,494 + BWI76_RS05660 0.11 +0.6
1,188,637 + BWI76_RS05660 0.37 +0.1
1,188,680 - BWI76_RS05660 0.45 +2.5
1,188,680 - BWI76_RS05660 0.45 +0.3
1,188,725 + BWI76_RS05660 0.53 -0.2
1,188,741 + BWI76_RS05660 0.56 +1.0
1,188,741 + BWI76_RS05660 0.56 +1.9
1,188,751 + BWI76_RS05660 0.58 +1.1
1,188,785 - BWI76_RS05660 0.64 -0.3
1,188,816 + BWI76_RS05660 0.70 +1.7
1,188,824 - BWI76_RS05660 0.71 +0.5
1,188,844 - BWI76_RS05660 0.75 +0.6
1,188,853 + BWI76_RS05660 0.76 +0.2
1,188,955 + -0.2
1,189,046 + +0.7
1,189,360 - BWI76_RS05665 0.22 -1.9
1,189,504 + BWI76_RS05665 0.31 +0.5
1,189,512 - BWI76_RS05665 0.31 -0.8
1,189,585 + BWI76_RS05665 0.36 +1.1
1,189,619 - BWI76_RS05665 0.38 +0.5
1,189,647 + BWI76_RS05665 0.40 -1.2
1,189,754 + BWI76_RS05665 0.46 +2.6
1,189,968 - BWI76_RS05665 0.59 -1.1

Or see this region's nucleotide sequence