Experiment: RCH2_defined_Glucose_noNitrogen in EcoFAB_3.5mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS03090 and BWI76_RS03095 are separated by 48 nucleotides BWI76_RS03095 and BWI76_RS03100 are separated by 41 nucleotides BWI76_RS03100 and BWI76_RS03105 are separated by 24 nucleotides
BWI76_RS03090: BWI76_RS03090 - 3D-(3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase IolD (EC 3.7.1.22) <small>(from data)</small>, at 635,179 to 637,119
_RS03090
BWI76_RS03095: BWI76_RS03095 - Inositol 2-dehydrogenase (EC 1.1.1.18) <small>(from data)</small>, at 637,168 to 638,181
_RS03095
BWI76_RS03100: BWI76_RS03100 - protein iolH, at 638,223 to 639,107
_RS03100
BWI76_RS03105: BWI76_RS03105 - myo-inosose-2 dehydratase, at 639,132 to 640,031
_RS03105
Position (kb) 637
638
639 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 636.326 kb on + strand, within BWI76_RS03090 at 636.425 kb on + strand, within BWI76_RS03090 at 636.610 kb on - strand, within BWI76_RS03090 at 636.627 kb on - strand, within BWI76_RS03090 at 636.701 kb on + strand, within BWI76_RS03090 at 636.701 kb on + strand, within BWI76_RS03090 at 636.701 kb on + strand, within BWI76_RS03090 at 636.701 kb on + strand, within BWI76_RS03090 at 636.701 kb on + strand, within BWI76_RS03090 at 636.703 kb on + strand, within BWI76_RS03090 at 636.709 kb on - strand, within BWI76_RS03090 at 636.709 kb on - strand, within BWI76_RS03090 at 636.709 kb on - strand, within BWI76_RS03090 at 636.848 kb on + strand, within BWI76_RS03090 at 636.985 kb on - strand at 637.410 kb on - strand, within BWI76_RS03095 at 637.439 kb on + strand, within BWI76_RS03095 at 637.923 kb on + strand, within BWI76_RS03095 at 638.203 kb on + strand at 638.221 kb on - strand at 638.257 kb on + strand at 638.597 kb on - strand, within BWI76_RS03100 at 638.782 kb on + strand, within BWI76_RS03100 at 638.801 kb on - strand, within BWI76_RS03100 at 638.887 kb on - strand, within BWI76_RS03100 at 638.951 kb on + strand, within BWI76_RS03100 at 638.959 kb on - strand, within BWI76_RS03100 at 639.037 kb on - strand at 639.070 kb on + strand at 639.071 kb on - strand at 639.071 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_Glucose_noNitrogen in EcoFAB_3.5mL remove 636,326 + BWI76_RS03090 0.59 +1.4 636,425 + BWI76_RS03090 0.64 -0.3 636,610 - BWI76_RS03090 0.74 -0.3 636,627 - BWI76_RS03090 0.75 +1.0 636,701 + BWI76_RS03090 0.78 -1.0 636,701 + BWI76_RS03090 0.78 -0.2 636,701 + BWI76_RS03090 0.78 -0.3 636,701 + BWI76_RS03090 0.78 +0.8 636,701 + BWI76_RS03090 0.78 -0.6 636,703 + BWI76_RS03090 0.79 +0.6 636,709 - BWI76_RS03090 0.79 +3.1 636,709 - BWI76_RS03090 0.79 +1.3 636,709 - BWI76_RS03090 0.79 +1.2 636,848 + BWI76_RS03090 0.86 -0.3 636,985 - +0.0 637,410 - BWI76_RS03095 0.24 +0.9 637,439 + BWI76_RS03095 0.27 -0.4 637,923 + BWI76_RS03095 0.74 -0.3 638,203 + +0.7 638,221 - +2.2 638,257 + +0.3 638,597 - BWI76_RS03100 0.42 -3.3 638,782 + BWI76_RS03100 0.63 -2.2 638,801 - BWI76_RS03100 0.65 -2.0 638,887 - BWI76_RS03100 0.75 -0.4 638,951 + BWI76_RS03100 0.82 -0.4 638,959 - BWI76_RS03100 0.83 +0.2 639,037 - +0.2 639,070 + -0.2 639,071 - +1.2 639,071 - -0.4
Or see this region's nucleotide sequence