Experiment: KB with Matrine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_4735 and Psyr_4736 overlap by 8 nucleotides Psyr_4736 and Psyr_4737 are separated by 109 nucleotides Psyr_4737 and Psyr_4738 are separated by 190 nucleotides
Psyr_4735: Psyr_4735 - GTP-binding protein, HSR1-related protein, at 5,613,980 to 5,615,368
_4735
Psyr_4736: Psyr_4736 - conserved hypothetical protein, at 5,615,361 to 5,616,740
_4736
Psyr_4737: Psyr_4737 - dihydrofolate reductase, at 5,616,850 to 5,617,362
_4737
Psyr_4738: Psyr_4738 - conserved hypothetical protein, at 5,617,553 to 5,618,611
_4738
Position (kb) 5615
5616
5617 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5614.527 kb on + strand, within Psyr_4735 at 5614.528 kb on - strand, within Psyr_4735 at 5614.956 kb on + strand, within Psyr_4735 at 5614.957 kb on - strand, within Psyr_4735 at 5614.992 kb on + strand, within Psyr_4735 at 5614.992 kb on + strand, within Psyr_4735 at 5614.992 kb on + strand, within Psyr_4735 at 5614.992 kb on + strand, within Psyr_4735 at 5614.992 kb on + strand, within Psyr_4735 at 5614.992 kb on + strand, within Psyr_4735 at 5614.992 kb on + strand, within Psyr_4735 at 5614.992 kb on + strand, within Psyr_4735 at 5614.993 kb on - strand, within Psyr_4735 at 5614.993 kb on - strand, within Psyr_4735 at 5614.993 kb on - strand, within Psyr_4735 at 5614.993 kb on - strand, within Psyr_4735 at 5614.993 kb on - strand, within Psyr_4735 at 5614.993 kb on - strand, within Psyr_4735 at 5615.178 kb on + strand, within Psyr_4735 at 5615.287 kb on + strand at 5615.287 kb on + strand at 5615.287 kb on + strand at 5615.288 kb on - strand at 5615.288 kb on - strand at 5615.288 kb on - strand at 5615.288 kb on - strand at 5615.317 kb on + strand at 5615.317 kb on + strand at 5615.654 kb on + strand, within Psyr_4736 at 5615.654 kb on + strand, within Psyr_4736 at 5615.724 kb on + strand, within Psyr_4736 at 5615.848 kb on + strand, within Psyr_4736 at 5616.350 kb on + strand, within Psyr_4736 at 5616.351 kb on - strand, within Psyr_4736 at 5616.775 kb on - strand at 5616.775 kb on - strand at 5617.441 kb on + strand at 5617.442 kb on - strand at 5617.442 kb on - strand at 5617.487 kb on - strand at 5617.487 kb on - strand at 5617.503 kb on + strand at 5617.504 kb on - strand at 5617.504 kb on - strand at 5617.553 kb on - strand at 5617.700 kb on + strand, within Psyr_4738 at 5617.701 kb on - strand, within Psyr_4738
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Matrine 10 mM remove 5,614,527 + Psyr_4735 0.39 +1.1 5,614,528 - Psyr_4735 0.39 +0.4 5,614,956 + Psyr_4735 0.70 +0.0 5,614,957 - Psyr_4735 0.70 -0.8 5,614,992 + Psyr_4735 0.73 +0.5 5,614,992 + Psyr_4735 0.73 -0.3 5,614,992 + Psyr_4735 0.73 -0.1 5,614,992 + Psyr_4735 0.73 +0.1 5,614,992 + Psyr_4735 0.73 +1.1 5,614,992 + Psyr_4735 0.73 -1.8 5,614,992 + Psyr_4735 0.73 -0.6 5,614,992 + Psyr_4735 0.73 -1.4 5,614,993 - Psyr_4735 0.73 +0.6 5,614,993 - Psyr_4735 0.73 -0.7 5,614,993 - Psyr_4735 0.73 +0.0 5,614,993 - Psyr_4735 0.73 -0.2 5,614,993 - Psyr_4735 0.73 +0.7 5,614,993 - Psyr_4735 0.73 +0.7 5,615,178 + Psyr_4735 0.86 -2.3 5,615,287 + +0.5 5,615,287 + +0.6 5,615,287 + -0.9 5,615,288 - -0.4 5,615,288 - -1.9 5,615,288 - +0.6 5,615,288 - -1.0 5,615,317 + -0.9 5,615,317 + +0.1 5,615,654 + Psyr_4736 0.21 -0.3 5,615,654 + Psyr_4736 0.21 -0.0 5,615,724 + Psyr_4736 0.26 -2.0 5,615,848 + Psyr_4736 0.35 +0.3 5,616,350 + Psyr_4736 0.72 -0.9 5,616,351 - Psyr_4736 0.72 -0.5 5,616,775 - +0.1 5,616,775 - +0.6 5,617,441 + -0.5 5,617,442 - +1.9 5,617,442 - -0.4 5,617,487 - -0.1 5,617,487 - -1.7 5,617,503 + +0.1 5,617,504 - +0.1 5,617,504 - -1.4 5,617,553 - -0.3 5,617,700 + Psyr_4738 0.14 +0.4 5,617,701 - Psyr_4738 0.14 -0.9
Or see this region's nucleotide sequence