Experiment: KB with Matrine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_4717 and Psyr_4718 are separated by 108 nucleotides Psyr_4718 and Psyr_4719 are separated by 31 nucleotides
Psyr_4717: Psyr_4717 - formyltetrahydrofolate deformylase, at 5,590,242 to 5,591,099
_4717
Psyr_4718: Psyr_4718 - glutathione-independent formaldehyde dehydrogenase, at 5,591,208 to 5,592,407
_4718
Psyr_4719: Psyr_4719 - hypothetical protein, at 5,592,439 to 5,593,485
_4719
Position (kb) 5591
5592
5593 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5590.224 kb on + strand at 5590.224 kb on + strand at 5590.241 kb on + strand at 5590.242 kb on - strand at 5590.242 kb on - strand at 5590.242 kb on - strand at 5590.242 kb on - strand at 5590.341 kb on + strand, within Psyr_4717 at 5590.341 kb on + strand, within Psyr_4717 at 5590.341 kb on + strand, within Psyr_4717 at 5590.341 kb on + strand, within Psyr_4717 at 5590.341 kb on + strand, within Psyr_4717 at 5590.341 kb on + strand, within Psyr_4717 at 5590.342 kb on - strand, within Psyr_4717 at 5590.342 kb on - strand, within Psyr_4717 at 5590.342 kb on - strand, within Psyr_4717 at 5590.342 kb on - strand, within Psyr_4717 at 5590.516 kb on + strand, within Psyr_4717 at 5590.516 kb on + strand, within Psyr_4717 at 5590.516 kb on + strand, within Psyr_4717 at 5590.517 kb on - strand, within Psyr_4717 at 5590.517 kb on - strand, within Psyr_4717 at 5590.574 kb on + strand, within Psyr_4717 at 5590.655 kb on + strand, within Psyr_4717 at 5590.655 kb on + strand, within Psyr_4717 at 5590.656 kb on - strand, within Psyr_4717 at 5590.656 kb on - strand, within Psyr_4717 at 5590.656 kb on - strand, within Psyr_4717 at 5590.659 kb on + strand, within Psyr_4717 at 5590.659 kb on + strand, within Psyr_4717 at 5590.659 kb on + strand, within Psyr_4717 at 5590.659 kb on + strand, within Psyr_4717 at 5590.659 kb on + strand, within Psyr_4717 at 5590.660 kb on - strand, within Psyr_4717 at 5590.660 kb on - strand, within Psyr_4717 at 5590.660 kb on - strand, within Psyr_4717 at 5590.660 kb on - strand, within Psyr_4717 at 5590.660 kb on - strand, within Psyr_4717 at 5590.660 kb on - strand, within Psyr_4717 at 5590.662 kb on + strand, within Psyr_4717 at 5590.662 kb on + strand, within Psyr_4717 at 5590.662 kb on + strand, within Psyr_4717 at 5590.663 kb on - strand, within Psyr_4717 at 5590.663 kb on - strand, within Psyr_4717 at 5590.761 kb on + strand, within Psyr_4717 at 5590.872 kb on + strand, within Psyr_4717 at 5590.872 kb on + strand, within Psyr_4717 at 5590.873 kb on - strand, within Psyr_4717 at 5590.873 kb on - strand, within Psyr_4717 at 5590.911 kb on + strand, within Psyr_4717 at 5590.911 kb on + strand, within Psyr_4717 at 5590.911 kb on + strand, within Psyr_4717 at 5590.911 kb on + strand, within Psyr_4717 at 5590.912 kb on - strand, within Psyr_4717 at 5590.912 kb on - strand, within Psyr_4717 at 5591.097 kb on + strand at 5591.128 kb on + strand at 5591.170 kb on + strand at 5591.170 kb on + strand at 5591.170 kb on + strand at 5591.171 kb on - strand at 5591.189 kb on + strand at 5591.189 kb on + strand at 5591.189 kb on + strand at 5591.190 kb on - strand at 5591.190 kb on - strand at 5591.207 kb on + strand at 5591.207 kb on + strand at 5591.207 kb on + strand at 5591.207 kb on + strand at 5591.207 kb on + strand at 5591.207 kb on + strand at 5591.207 kb on + strand at 5591.207 kb on + strand at 5591.207 kb on + strand at 5591.207 kb on + strand at 5591.207 kb on + strand at 5591.207 kb on + strand at 5591.207 kb on + strand at 5591.207 kb on + strand at 5591.207 kb on + strand at 5591.207 kb on + strand at 5591.207 kb on + strand at 5591.207 kb on + strand at 5591.207 kb on + strand at 5591.207 kb on + strand at 5591.207 kb on + strand at 5591.207 kb on + strand at 5591.207 kb on + strand at 5591.207 kb on + strand at 5591.207 kb on + strand at 5591.207 kb on + strand at 5591.207 kb on + strand at 5591.207 kb on + strand at 5591.207 kb on + strand at 5591.207 kb on + strand at 5591.207 kb on + strand at 5591.207 kb on + strand at 5591.207 kb on + strand at 5591.207 kb on + strand at 5591.207 kb on + strand at 5591.207 kb on + strand at 5591.207 kb on + strand at 5591.207 kb on + strand at 5591.207 kb on + strand at 5591.207 kb on + strand at 5591.207 kb on + strand at 5591.207 kb on + strand at 5591.207 kb on + strand at 5591.208 kb on - strand at 5591.208 kb on - strand at 5591.208 kb on - strand at 5591.208 kb on - strand at 5591.208 kb on - strand at 5591.208 kb on - strand at 5591.208 kb on - strand at 5591.208 kb on - strand at 5591.208 kb on - strand at 5591.208 kb on - strand at 5591.208 kb on - strand at 5591.208 kb on - strand at 5591.208 kb on - strand at 5591.208 kb on - strand at 5591.208 kb on - strand at 5591.208 kb on - strand at 5591.208 kb on - strand at 5591.208 kb on - strand at 5591.208 kb on - strand at 5591.208 kb on - strand at 5591.208 kb on - strand at 5591.208 kb on - strand at 5591.208 kb on - strand at 5591.208 kb on - strand at 5591.208 kb on - strand at 5591.208 kb on - strand at 5591.208 kb on - strand at 5591.208 kb on - strand at 5591.208 kb on - strand at 5591.208 kb on - strand at 5591.208 kb on - strand at 5591.208 kb on - strand at 5591.208 kb on - strand at 5591.208 kb on - strand at 5591.208 kb on - strand at 5591.208 kb on - strand at 5591.208 kb on - strand at 5591.208 kb on - strand at 5591.208 kb on - strand at 5591.208 kb on - strand at 5591.208 kb on - strand at 5591.227 kb on + strand at 5591.227 kb on + strand at 5591.227 kb on + strand at 5591.227 kb on + strand at 5591.228 kb on - strand at 5591.232 kb on + strand at 5591.232 kb on + strand at 5591.232 kb on + strand at 5591.233 kb on - strand at 5591.233 kb on - strand at 5591.369 kb on - strand, within Psyr_4718 at 5591.577 kb on + strand, within Psyr_4718 at 5591.577 kb on + strand, within Psyr_4718 at 5591.577 kb on + strand, within Psyr_4718 at 5591.577 kb on + strand, within Psyr_4718 at 5591.577 kb on + strand, within Psyr_4718 at 5591.583 kb on + strand, within Psyr_4718 at 5591.583 kb on + strand, within Psyr_4718 at 5591.583 kb on + strand, within Psyr_4718 at 5591.583 kb on + strand, within Psyr_4718 at 5591.584 kb on - strand, within Psyr_4718 at 5591.584 kb on - strand, within Psyr_4718 at 5591.584 kb on - strand, within Psyr_4718 at 5591.584 kb on - strand, within Psyr_4718 at 5591.584 kb on - strand, within Psyr_4718 at 5591.622 kb on + strand, within Psyr_4718 at 5591.622 kb on + strand, within Psyr_4718 at 5591.622 kb on + strand, within Psyr_4718 at 5591.622 kb on + strand, within Psyr_4718 at 5591.622 kb on + strand, within Psyr_4718 at 5591.622 kb on + strand, within Psyr_4718 at 5591.623 kb on - strand, within Psyr_4718 at 5591.623 kb on - strand, within Psyr_4718 at 5591.623 kb on - strand, within Psyr_4718 at 5591.778 kb on + strand, within Psyr_4718 at 5591.778 kb on + strand, within Psyr_4718 at 5591.778 kb on + strand, within Psyr_4718 at 5591.778 kb on + strand, within Psyr_4718 at 5591.778 kb on + strand, within Psyr_4718 at 5591.779 kb on - strand, within Psyr_4718 at 5591.996 kb on - strand, within Psyr_4718 at 5591.996 kb on - strand, within Psyr_4718 at 5592.076 kb on + strand, within Psyr_4718 at 5592.077 kb on - strand, within Psyr_4718 at 5592.114 kb on + strand, within Psyr_4718 at 5592.114 kb on + strand, within Psyr_4718 at 5592.114 kb on + strand, within Psyr_4718 at 5592.115 kb on - strand, within Psyr_4718 at 5592.115 kb on - strand, within Psyr_4718 at 5592.115 kb on - strand, within Psyr_4718 at 5592.115 kb on - strand, within Psyr_4718 at 5592.115 kb on - strand, within Psyr_4718 at 5592.115 kb on - strand, within Psyr_4718 at 5592.115 kb on - strand, within Psyr_4718 at 5592.237 kb on + strand, within Psyr_4718 at 5592.237 kb on + strand, within Psyr_4718 at 5592.237 kb on + strand, within Psyr_4718 at 5592.238 kb on - strand, within Psyr_4718 at 5592.238 kb on - strand, within Psyr_4718 at 5592.238 kb on - strand, within Psyr_4718 at 5592.238 kb on - strand, within Psyr_4718 at 5592.238 kb on - strand, within Psyr_4718 at 5592.292 kb on + strand at 5592.293 kb on - strand at 5592.293 kb on - strand at 5592.293 kb on - strand at 5592.293 kb on - strand at 5592.405 kb on + strand at 5592.419 kb on - strand at 5592.440 kb on + strand at 5592.440 kb on + strand at 5592.440 kb on + strand at 5592.441 kb on - strand at 5592.441 kb on - strand at 5592.441 kb on - strand at 5592.441 kb on - strand at 5592.441 kb on - strand at 5592.596 kb on + strand, within Psyr_4719 at 5592.596 kb on + strand, within Psyr_4719 at 5592.612 kb on + strand, within Psyr_4719 at 5592.612 kb on + strand, within Psyr_4719 at 5592.614 kb on + strand, within Psyr_4719 at 5592.614 kb on + strand, within Psyr_4719 at 5592.642 kb on - strand, within Psyr_4719 at 5592.712 kb on - strand, within Psyr_4719 at 5592.724 kb on - strand, within Psyr_4719 at 5592.844 kb on - strand, within Psyr_4719 at 5592.848 kb on - strand, within Psyr_4719 at 5592.848 kb on - strand, within Psyr_4719 at 5592.848 kb on - strand, within Psyr_4719 at 5592.931 kb on + strand, within Psyr_4719 at 5592.972 kb on + strand, within Psyr_4719 at 5593.022 kb on + strand, within Psyr_4719 at 5593.071 kb on + strand at 5593.093 kb on - strand, within Psyr_4719 at 5593.093 kb on - strand, within Psyr_4719 at 5593.121 kb on + strand, within Psyr_4719 at 5593.121 kb on + strand, within Psyr_4719 at 5593.122 kb on - strand, within Psyr_4719 at 5593.122 kb on - strand, within Psyr_4719 at 5593.209 kb on - strand, within Psyr_4719 at 5593.329 kb on + strand, within Psyr_4719 at 5593.399 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Matrine 10 mM remove 5,590,224 + +1.0 5,590,224 + +0.2 5,590,241 + -2.0 5,590,242 - +0.1 5,590,242 - +0.4 5,590,242 - +0.2 5,590,242 - -0.1 5,590,341 + Psyr_4717 0.12 -0.8 5,590,341 + Psyr_4717 0.12 -0.4 5,590,341 + Psyr_4717 0.12 -0.4 5,590,341 + Psyr_4717 0.12 -0.2 5,590,341 + Psyr_4717 0.12 -0.8 5,590,341 + Psyr_4717 0.12 -0.6 5,590,342 - Psyr_4717 0.12 +0.3 5,590,342 - Psyr_4717 0.12 +0.7 5,590,342 - Psyr_4717 0.12 -0.2 5,590,342 - Psyr_4717 0.12 -0.6 5,590,516 + Psyr_4717 0.32 -0.5 5,590,516 + Psyr_4717 0.32 +1.2 5,590,516 + Psyr_4717 0.32 -0.3 5,590,517 - Psyr_4717 0.32 +1.6 5,590,517 - Psyr_4717 0.32 +0.3 5,590,574 + Psyr_4717 0.39 -0.3 5,590,655 + Psyr_4717 0.48 -0.6 5,590,655 + Psyr_4717 0.48 -0.3 5,590,656 - Psyr_4717 0.48 -1.5 5,590,656 - Psyr_4717 0.48 -0.9 5,590,656 - Psyr_4717 0.48 +0.5 5,590,659 + Psyr_4717 0.49 -1.0 5,590,659 + Psyr_4717 0.49 -0.5 5,590,659 + Psyr_4717 0.49 -1.0 5,590,659 + Psyr_4717 0.49 +0.6 5,590,659 + Psyr_4717 0.49 +0.6 5,590,660 - Psyr_4717 0.49 +1.1 5,590,660 - Psyr_4717 0.49 +0.3 5,590,660 - Psyr_4717 0.49 -0.2 5,590,660 - Psyr_4717 0.49 +0.3 5,590,660 - Psyr_4717 0.49 -0.4 5,590,660 - Psyr_4717 0.49 -0.6 5,590,662 + Psyr_4717 0.49 +0.6 5,590,662 + Psyr_4717 0.49 +0.4 5,590,662 + Psyr_4717 0.49 -1.2 5,590,663 - Psyr_4717 0.49 -0.4 5,590,663 - Psyr_4717 0.49 -0.1 5,590,761 + Psyr_4717 0.60 +0.3 5,590,872 + Psyr_4717 0.73 -0.4 5,590,872 + Psyr_4717 0.73 +0.0 5,590,873 - Psyr_4717 0.74 +0.3 5,590,873 - Psyr_4717 0.74 -1.5 5,590,911 + Psyr_4717 0.78 +0.9 5,590,911 + Psyr_4717 0.78 +1.0 5,590,911 + Psyr_4717 0.78 +0.0 5,590,911 + Psyr_4717 0.78 -1.4 5,590,912 - Psyr_4717 0.78 +0.5 5,590,912 - Psyr_4717 0.78 -0.6 5,591,097 + -0.1 5,591,128 + +0.3 5,591,170 + +0.3 5,591,170 + +0.3 5,591,170 + +0.9 5,591,171 - +0.4 5,591,189 + -0.0 5,591,189 + +1.2 5,591,189 + +1.6 5,591,190 - -0.3 5,591,190 - +0.3 5,591,207 + +0.1 5,591,207 + +0.3 5,591,207 + +0.3 5,591,207 + -1.9 5,591,207 + +2.1 5,591,207 + +0.3 5,591,207 + +0.1 5,591,207 + +0.2 5,591,207 + -0.4 5,591,207 + -0.3 5,591,207 + +0.5 5,591,207 + +0.3 5,591,207 + -0.1 5,591,207 + -0.0 5,591,207 + +0.4 5,591,207 + +1.3 5,591,207 + -2.4 5,591,207 + -0.2 5,591,207 + -0.0 5,591,207 + +1.4 5,591,207 + +0.6 5,591,207 + -1.1 5,591,207 + -0.7 5,591,207 + +1.3 5,591,207 + -0.3 5,591,207 + -0.5 5,591,207 + -0.1 5,591,207 + -1.2 5,591,207 + -0.3 5,591,207 + +0.3 5,591,207 + -0.4 5,591,207 + +0.3 5,591,207 + +0.3 5,591,207 + +1.5 5,591,207 + +0.7 5,591,207 + -0.2 5,591,207 + -0.6 5,591,207 + -1.3 5,591,207 + -0.3 5,591,207 + -0.2 5,591,207 + +0.2 5,591,207 + -2.4 5,591,207 + +0.3 5,591,208 - -0.5 5,591,208 - -1.3 5,591,208 - +2.1 5,591,208 - -0.3 5,591,208 - -0.2 5,591,208 - +0.2 5,591,208 - +0.4 5,591,208 - +0.8 5,591,208 - +1.8 5,591,208 - +1.2 5,591,208 - -0.2 5,591,208 - +0.1 5,591,208 - +0.4 5,591,208 - -0.2 5,591,208 - +0.4 5,591,208 - -1.2 5,591,208 - -2.0 5,591,208 - +0.2 5,591,208 - +0.3 5,591,208 - +0.3 5,591,208 - -0.3 5,591,208 - +1.7 5,591,208 - +0.7 5,591,208 - +0.7 5,591,208 - +0.9 5,591,208 - +1.0 5,591,208 - +0.2 5,591,208 - +0.2 5,591,208 - +0.8 5,591,208 - +1.3 5,591,208 - -0.3 5,591,208 - +1.0 5,591,208 - +0.9 5,591,208 - -0.1 5,591,208 - -2.0 5,591,208 - +1.0 5,591,208 - -0.3 5,591,208 - -1.0 5,591,208 - -0.8 5,591,208 - -0.1 5,591,208 - -0.3 5,591,227 + -1.0 5,591,227 + +0.7 5,591,227 + +0.1 5,591,227 + -0.2 5,591,228 - +0.1 5,591,232 + -1.9 5,591,232 + -0.1 5,591,232 + -1.1 5,591,233 - +0.6 5,591,233 - -0.2 5,591,369 - Psyr_4718 0.13 -0.2 5,591,577 + Psyr_4718 0.31 -1.1 5,591,577 + Psyr_4718 0.31 +1.1 5,591,577 + Psyr_4718 0.31 +0.9 5,591,577 + Psyr_4718 0.31 -1.7 5,591,577 + Psyr_4718 0.31 -0.3 5,591,583 + Psyr_4718 0.31 +0.3 5,591,583 + Psyr_4718 0.31 -0.7 5,591,583 + Psyr_4718 0.31 +0.2 5,591,583 + Psyr_4718 0.31 -0.5 5,591,584 - Psyr_4718 0.31 +2.6 5,591,584 - Psyr_4718 0.31 -0.7 5,591,584 - Psyr_4718 0.31 -0.3 5,591,584 - Psyr_4718 0.31 +0.9 5,591,584 - Psyr_4718 0.31 -0.2 5,591,622 + Psyr_4718 0.34 +0.2 5,591,622 + Psyr_4718 0.34 -0.2 5,591,622 + Psyr_4718 0.34 -0.4 5,591,622 + Psyr_4718 0.34 -0.3 5,591,622 + Psyr_4718 0.34 -1.3 5,591,622 + Psyr_4718 0.34 +0.3 5,591,623 - Psyr_4718 0.35 +0.5 5,591,623 - Psyr_4718 0.35 +0.2 5,591,623 - Psyr_4718 0.35 +1.1 5,591,778 + Psyr_4718 0.47 +0.7 5,591,778 + Psyr_4718 0.47 +0.1 5,591,778 + Psyr_4718 0.47 +0.1 5,591,778 + Psyr_4718 0.47 +0.1 5,591,778 + Psyr_4718 0.47 +0.2 5,591,779 - Psyr_4718 0.48 -0.0 5,591,996 - Psyr_4718 0.66 -0.2 5,591,996 - Psyr_4718 0.66 -0.8 5,592,076 + Psyr_4718 0.72 -2.0 5,592,077 - Psyr_4718 0.72 -0.3 5,592,114 + Psyr_4718 0.76 -0.1 5,592,114 + Psyr_4718 0.76 -0.6 5,592,114 + Psyr_4718 0.76 -0.5 5,592,115 - Psyr_4718 0.76 -0.2 5,592,115 - Psyr_4718 0.76 -0.9 5,592,115 - Psyr_4718 0.76 -1.1 5,592,115 - Psyr_4718 0.76 -0.7 5,592,115 - Psyr_4718 0.76 +1.0 5,592,115 - Psyr_4718 0.76 -0.1 5,592,115 - Psyr_4718 0.76 +0.8 5,592,237 + Psyr_4718 0.86 -0.5 5,592,237 + Psyr_4718 0.86 +0.3 5,592,237 + Psyr_4718 0.86 -0.0 5,592,238 - Psyr_4718 0.86 +1.3 5,592,238 - Psyr_4718 0.86 +0.5 5,592,238 - Psyr_4718 0.86 +0.5 5,592,238 - Psyr_4718 0.86 -1.3 5,592,238 - Psyr_4718 0.86 -0.7 5,592,292 + +0.3 5,592,293 - -0.0 5,592,293 - +0.5 5,592,293 - -0.9 5,592,293 - -0.3 5,592,405 + -0.7 5,592,419 - -2.3 5,592,440 + -0.8 5,592,440 + +0.1 5,592,440 + -0.7 5,592,441 - +0.1 5,592,441 - -1.1 5,592,441 - -0.1 5,592,441 - +0.7 5,592,441 - +0.9 5,592,596 + Psyr_4719 0.15 -0.3 5,592,596 + Psyr_4719 0.15 -1.2 5,592,612 + Psyr_4719 0.17 -2.7 5,592,612 + Psyr_4719 0.17 +0.9 5,592,614 + Psyr_4719 0.17 -0.4 5,592,614 + Psyr_4719 0.17 +0.5 5,592,642 - Psyr_4719 0.19 -0.4 5,592,712 - Psyr_4719 0.26 +0.1 5,592,724 - Psyr_4719 0.27 +0.6 5,592,844 - Psyr_4719 0.39 -0.4 5,592,848 - Psyr_4719 0.39 +0.5 5,592,848 - Psyr_4719 0.39 +0.5 5,592,848 - Psyr_4719 0.39 +0.3 5,592,931 + Psyr_4719 0.47 -0.0 5,592,972 + Psyr_4719 0.51 -0.7 5,593,022 + Psyr_4719 0.56 -0.4 5,593,071 + +1.3 5,593,093 - Psyr_4719 0.62 -0.4 5,593,093 - Psyr_4719 0.62 -1.7 5,593,121 + Psyr_4719 0.65 +0.4 5,593,121 + Psyr_4719 0.65 -0.2 5,593,122 - Psyr_4719 0.65 +0.9 5,593,122 - Psyr_4719 0.65 +0.3 5,593,209 - Psyr_4719 0.74 +0.2 5,593,329 + Psyr_4719 0.85 -0.6 5,593,399 - +0.6
Or see this region's nucleotide sequence