Experiment: KB with Matrine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_4198 and Psyr_4199 are separated by 98 nucleotides Psyr_4199 and Psyr_4200 are separated by 63 nucleotides Psyr_4200 and Psyr_4201 overlap by 8 nucleotides Psyr_4201 and Psyr_4202 are separated by 7 nucleotides
Psyr_4198: Psyr_4198 - Ferric-uptake regulator, at 4,995,282 to 4,995,689
_4198
Psyr_4199: Psyr_4199 - SmpA/OmlA, at 4,995,788 to 4,996,309
_4199
Psyr_4200: Psyr_4200 - Protein of unknown function UPF0125, at 4,996,373 to 4,996,687
_4200
Psyr_4201: Psyr_4201 - cyclase/dehydrase, at 4,996,680 to 4,997,114
_4201
Psyr_4202: Psyr_4202 - Sodium:neurotransmitter symporter, at 4,997,122 to 4,998,558
_4202
Position (kb)
4996
4997 Strain fitness (log2 ratio)
-2
-1
0
1 at 4996.455 kb on + strand, within Psyr_4200 at 4996.456 kb on - strand, within Psyr_4200 at 4996.523 kb on - strand, within Psyr_4200 at 4996.523 kb on - strand, within Psyr_4200 at 4996.613 kb on + strand, within Psyr_4200 at 4996.614 kb on - strand, within Psyr_4200 at 4996.632 kb on - strand, within Psyr_4200 at 4996.632 kb on - strand, within Psyr_4200 at 4996.632 kb on - strand, within Psyr_4200 at 4996.643 kb on + strand, within Psyr_4200 at 4996.644 kb on - strand, within Psyr_4200 at 4996.653 kb on + strand, within Psyr_4200 at 4996.653 kb on + strand, within Psyr_4200 at 4996.654 kb on - strand, within Psyr_4200 at 4996.654 kb on - strand, within Psyr_4200 at 4996.654 kb on - strand, within Psyr_4200 at 4996.664 kb on + strand at 4996.665 kb on - strand at 4997.101 kb on - strand at 4997.175 kb on - strand at 4997.270 kb on - strand, within Psyr_4202 at 4997.270 kb on - strand, within Psyr_4202 at 4997.272 kb on + strand, within Psyr_4202 at 4997.272 kb on + strand, within Psyr_4202 at 4997.272 kb on + strand, within Psyr_4202 at 4997.273 kb on - strand, within Psyr_4202 at 4997.273 kb on - strand, within Psyr_4202 at 4997.273 kb on - strand, within Psyr_4202 at 4997.367 kb on - strand, within Psyr_4202 at 4997.367 kb on - strand, within Psyr_4202 at 4997.367 kb on - strand, within Psyr_4202 at 4997.418 kb on + strand, within Psyr_4202 at 4997.418 kb on + strand, within Psyr_4202 at 4997.418 kb on + strand, within Psyr_4202 at 4997.419 kb on - strand, within Psyr_4202 at 4997.461 kb on - strand, within Psyr_4202 at 4997.496 kb on + strand, within Psyr_4202 at 4997.517 kb on - strand, within Psyr_4202 at 4997.537 kb on - strand, within Psyr_4202 at 4997.576 kb on - strand, within Psyr_4202 at 4997.628 kb on - strand, within Psyr_4202
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Matrine 10 mM remove 4,996,455 + Psyr_4200 0.26 -0.2 4,996,456 - Psyr_4200 0.26 -1.0 4,996,523 - Psyr_4200 0.48 +1.0 4,996,523 - Psyr_4200 0.48 -1.3 4,996,613 + Psyr_4200 0.76 -1.5 4,996,614 - Psyr_4200 0.77 +1.5 4,996,632 - Psyr_4200 0.82 -1.6 4,996,632 - Psyr_4200 0.82 +0.2 4,996,632 - Psyr_4200 0.82 -0.5 4,996,643 + Psyr_4200 0.86 +0.6 4,996,644 - Psyr_4200 0.86 -0.8 4,996,653 + Psyr_4200 0.89 -1.5 4,996,653 + Psyr_4200 0.89 +0.0 4,996,654 - Psyr_4200 0.89 -0.1 4,996,654 - Psyr_4200 0.89 +0.8 4,996,654 - Psyr_4200 0.89 -0.6 4,996,664 + -0.9 4,996,665 - +0.8 4,997,101 - +0.3 4,997,175 - +0.4 4,997,270 - Psyr_4202 0.10 -1.4 4,997,270 - Psyr_4202 0.10 -0.0 4,997,272 + Psyr_4202 0.10 +0.6 4,997,272 + Psyr_4202 0.10 +0.3 4,997,272 + Psyr_4202 0.10 +0.8 4,997,273 - Psyr_4202 0.11 -2.1 4,997,273 - Psyr_4202 0.11 -0.3 4,997,273 - Psyr_4202 0.11 +1.2 4,997,367 - Psyr_4202 0.17 +1.7 4,997,367 - Psyr_4202 0.17 -0.8 4,997,367 - Psyr_4202 0.17 +0.3 4,997,418 + Psyr_4202 0.21 +1.3 4,997,418 + Psyr_4202 0.21 -0.8 4,997,418 + Psyr_4202 0.21 -0.9 4,997,419 - Psyr_4202 0.21 +0.3 4,997,461 - Psyr_4202 0.24 +0.9 4,997,496 + Psyr_4202 0.26 -0.2 4,997,517 - Psyr_4202 0.27 -0.2 4,997,537 - Psyr_4202 0.29 -1.0 4,997,576 - Psyr_4202 0.32 -1.5 4,997,628 - Psyr_4202 0.35 -0.4
Or see this region's nucleotide sequence