Experiment: KB with Matrine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2736 and Psyr_2737 are separated by 164 nucleotides Psyr_2737 and Psyr_2738 are separated by 50 nucleotides Psyr_2738 and Psyr_2739 are separated by 202 nucleotides Psyr_2739 and Psyr_2740 are separated by 142 nucleotides
Psyr_2736: Psyr_2736 - glycosidase, putative, at 3,321,952 to 3,324,015
_2736
Psyr_2737: Psyr_2737 - hypothetical protein, at 3,324,180 to 3,324,695
_2737
Psyr_2738: Psyr_2738 - regulatory protein, LysR, at 3,324,746 to 3,325,069
_2738
Psyr_2739: Psyr_2739 - ThiJ/PfpI, at 3,325,272 to 3,325,877
_2739
Psyr_2740: Psyr_2740 - transcriptional regulator, AraC family with amidase-like domain protein, at 3,326,020 to 3,326,973
_2740
Position (kb)
3324
3325
3326 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3323.993 kb on + strand at 3323.993 kb on + strand at 3323.994 kb on - strand at 3323.994 kb on - strand at 3324.079 kb on + strand at 3324.079 kb on + strand at 3324.079 kb on + strand at 3324.079 kb on + strand at 3324.079 kb on + strand at 3324.080 kb on - strand at 3324.080 kb on - strand at 3324.080 kb on - strand at 3324.080 kb on - strand at 3324.080 kb on - strand at 3324.080 kb on - strand at 3324.080 kb on - strand at 3324.435 kb on + strand, within Psyr_2737 at 3324.496 kb on + strand, within Psyr_2737 at 3324.496 kb on + strand, within Psyr_2737 at 3324.564 kb on + strand, within Psyr_2737 at 3324.565 kb on - strand, within Psyr_2737 at 3324.565 kb on - strand, within Psyr_2737 at 3324.568 kb on + strand, within Psyr_2737 at 3324.569 kb on - strand, within Psyr_2737 at 3324.598 kb on + strand, within Psyr_2737 at 3324.652 kb on - strand at 3324.835 kb on + strand, within Psyr_2738 at 3324.835 kb on + strand, within Psyr_2738 at 3324.835 kb on + strand, within Psyr_2738 at 3324.835 kb on + strand, within Psyr_2738 at 3324.835 kb on + strand, within Psyr_2738 at 3324.836 kb on - strand, within Psyr_2738 at 3324.836 kb on - strand, within Psyr_2738 at 3324.836 kb on - strand, within Psyr_2738 at 3324.945 kb on + strand, within Psyr_2738 at 3324.946 kb on - strand, within Psyr_2738 at 3324.946 kb on - strand, within Psyr_2738 at 3324.946 kb on - strand, within Psyr_2738 at 3325.110 kb on + strand at 3325.110 kb on + strand at 3325.237 kb on + strand at 3325.237 kb on + strand at 3325.237 kb on + strand at 3325.238 kb on - strand at 3325.238 kb on - strand at 3325.238 kb on - strand at 3325.238 kb on - strand at 3325.313 kb on + strand at 3325.382 kb on + strand, within Psyr_2739 at 3325.383 kb on - strand, within Psyr_2739 at 3325.806 kb on + strand, within Psyr_2739 at 3325.806 kb on + strand, within Psyr_2739 at 3325.806 kb on + strand, within Psyr_2739 at 3325.806 kb on + strand, within Psyr_2739 at 3325.806 kb on + strand, within Psyr_2739 at 3325.807 kb on - strand, within Psyr_2739 at 3325.807 kb on - strand, within Psyr_2739 at 3325.855 kb on + strand at 3325.855 kb on + strand at 3325.855 kb on + strand at 3325.856 kb on - strand at 3325.856 kb on - strand at 3325.856 kb on - strand at 3325.856 kb on - strand at 3325.856 kb on - strand at 3325.859 kb on + strand at 3325.918 kb on + strand at 3325.919 kb on - strand at 3325.919 kb on - strand at 3325.919 kb on - strand at 3325.960 kb on + strand at 3325.984 kb on - strand at 3326.050 kb on + strand at 3326.050 kb on + strand at 3326.050 kb on + strand at 3326.050 kb on + strand at 3326.050 kb on + strand at 3326.051 kb on - strand at 3326.051 kb on - strand at 3326.051 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Matrine 10 mM remove 3,323,993 + +0.3 3,323,993 + -0.5 3,323,994 - +0.3 3,323,994 - +0.6 3,324,079 + +0.4 3,324,079 + -0.2 3,324,079 + -0.2 3,324,079 + -0.7 3,324,079 + +1.1 3,324,080 - +1.0 3,324,080 - +2.3 3,324,080 - -0.5 3,324,080 - -0.9 3,324,080 - +0.7 3,324,080 - +0.4 3,324,080 - +0.7 3,324,435 + Psyr_2737 0.49 -0.5 3,324,496 + Psyr_2737 0.61 -0.3 3,324,496 + Psyr_2737 0.61 +1.3 3,324,564 + Psyr_2737 0.74 -0.1 3,324,565 - Psyr_2737 0.75 -1.2 3,324,565 - Psyr_2737 0.75 -0.1 3,324,568 + Psyr_2737 0.75 -0.6 3,324,569 - Psyr_2737 0.75 +1.3 3,324,598 + Psyr_2737 0.81 +1.1 3,324,652 - +0.4 3,324,835 + Psyr_2738 0.27 -0.6 3,324,835 + Psyr_2738 0.27 -0.5 3,324,835 + Psyr_2738 0.27 -1.0 3,324,835 + Psyr_2738 0.27 +0.5 3,324,835 + Psyr_2738 0.27 +1.3 3,324,836 - Psyr_2738 0.28 -1.0 3,324,836 - Psyr_2738 0.28 -1.0 3,324,836 - Psyr_2738 0.28 -1.4 3,324,945 + Psyr_2738 0.61 -0.6 3,324,946 - Psyr_2738 0.62 -0.8 3,324,946 - Psyr_2738 0.62 -0.4 3,324,946 - Psyr_2738 0.62 -0.7 3,325,110 + +0.5 3,325,110 + -0.7 3,325,237 + -2.8 3,325,237 + -0.2 3,325,237 + +1.6 3,325,238 - -0.0 3,325,238 - +0.2 3,325,238 - -0.0 3,325,238 - +0.8 3,325,313 + -1.0 3,325,382 + Psyr_2739 0.18 +2.5 3,325,383 - Psyr_2739 0.18 -0.5 3,325,806 + Psyr_2739 0.88 -0.6 3,325,806 + Psyr_2739 0.88 +1.0 3,325,806 + Psyr_2739 0.88 -0.1 3,325,806 + Psyr_2739 0.88 -0.3 3,325,806 + Psyr_2739 0.88 +0.1 3,325,807 - Psyr_2739 0.88 -2.9 3,325,807 - Psyr_2739 0.88 -1.0 3,325,855 + -0.6 3,325,855 + -0.0 3,325,855 + +0.7 3,325,856 - -0.9 3,325,856 - -0.2 3,325,856 - +2.6 3,325,856 - -0.3 3,325,856 - +3.6 3,325,859 + -0.2 3,325,918 + +0.9 3,325,919 - +0.2 3,325,919 - +1.1 3,325,919 - +0.1 3,325,960 + +0.1 3,325,984 - +0.3 3,326,050 + -0.2 3,326,050 + -0.1 3,326,050 + +0.4 3,326,050 + +0.9 3,326,050 + +1.3 3,326,051 - +2.4 3,326,051 - +0.4 3,326,051 - -1.2
Or see this region's nucleotide sequence