Experiment: KB with Matrine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2349 and Psyr_2350 are separated by 411 nucleotides Psyr_2350 and Psyr_2351 are separated by 78 nucleotides Psyr_2351 and Psyr_2352 are separated by 289 nucleotides Psyr_2352 and Psyr_2353 are separated by 115 nucleotides
Psyr_2349: Psyr_2349 - hypothetical protein, at 2,725,826 to 2,726,128
_2349
Psyr_2350: Psyr_2350 - Protein of unknown function UPF0025, at 2,726,540 to 2,726,998
_2350
Psyr_2351: Psyr_2351 - tRNA synthetase, class-II (G, H, P and S), at 2,727,077 to 2,727,658
_2351
Psyr_2352: Psyr_2352 - GCN5-related N-acetyltransferase, at 2,727,948 to 2,728,475
_2352
Psyr_2353: Psyr_2353 - Uncharacterized protein conserved in bacteria-like protein, at 2,728,591 to 2,729,817
_2353
Position (kb)
2727
2728 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 2726.126 kb on + strand at 2726.126 kb on + strand at 2726.168 kb on - strand at 2726.168 kb on - strand at 2726.173 kb on + strand at 2726.173 kb on + strand at 2726.174 kb on - strand at 2726.180 kb on + strand at 2726.180 kb on + strand at 2726.180 kb on + strand at 2726.180 kb on + strand at 2726.181 kb on - strand at 2726.181 kb on - strand at 2726.181 kb on - strand at 2726.181 kb on - strand at 2726.181 kb on - strand at 2726.181 kb on - strand at 2726.210 kb on + strand at 2726.211 kb on - strand at 2726.236 kb on - strand at 2726.242 kb on + strand at 2726.268 kb on - strand at 2726.288 kb on + strand at 2726.426 kb on + strand at 2726.426 kb on + strand at 2726.426 kb on + strand at 2726.431 kb on - strand at 2726.451 kb on + strand at 2726.451 kb on + strand at 2726.451 kb on + strand at 2726.451 kb on + strand at 2726.451 kb on + strand at 2726.451 kb on + strand at 2726.451 kb on + strand at 2726.452 kb on - strand at 2726.452 kb on - strand at 2726.452 kb on - strand at 2726.452 kb on - strand at 2726.452 kb on - strand at 2726.452 kb on - strand at 2726.452 kb on - strand at 2726.519 kb on + strand at 2726.594 kb on + strand, within Psyr_2350 at 2726.652 kb on + strand, within Psyr_2350 at 2726.652 kb on + strand, within Psyr_2350 at 2726.652 kb on + strand, within Psyr_2350 at 2726.653 kb on - strand, within Psyr_2350 at 2726.653 kb on - strand, within Psyr_2350 at 2727.026 kb on - strand at 2727.026 kb on - strand at 2727.039 kb on - strand at 2727.055 kb on - strand at 2727.064 kb on + strand at 2727.101 kb on + strand at 2727.219 kb on + strand, within Psyr_2351 at 2727.219 kb on + strand, within Psyr_2351 at 2727.220 kb on - strand, within Psyr_2351 at 2727.222 kb on + strand, within Psyr_2351 at 2727.222 kb on + strand, within Psyr_2351 at 2727.222 kb on + strand, within Psyr_2351 at 2727.222 kb on + strand, within Psyr_2351 at 2727.222 kb on + strand, within Psyr_2351 at 2727.223 kb on - strand, within Psyr_2351 at 2727.223 kb on - strand, within Psyr_2351 at 2727.259 kb on - strand, within Psyr_2351 at 2727.631 kb on + strand at 2727.631 kb on + strand at 2727.632 kb on - strand at 2727.632 kb on - strand at 2727.632 kb on - strand at 2727.702 kb on + strand at 2727.743 kb on + strand at 2727.926 kb on + strand at 2727.926 kb on + strand at 2727.949 kb on + strand at 2727.950 kb on - strand at 2728.003 kb on + strand, within Psyr_2352 at 2728.075 kb on + strand, within Psyr_2352 at 2728.075 kb on + strand, within Psyr_2352 at 2728.075 kb on + strand, within Psyr_2352 at 2728.075 kb on + strand, within Psyr_2352 at 2728.076 kb on - strand, within Psyr_2352 at 2728.076 kb on - strand, within Psyr_2352 at 2728.225 kb on + strand, within Psyr_2352 at 2728.225 kb on + strand, within Psyr_2352 at 2728.378 kb on + strand, within Psyr_2352 at 2728.378 kb on + strand, within Psyr_2352 at 2728.378 kb on + strand, within Psyr_2352 at 2728.378 kb on + strand, within Psyr_2352 at 2728.379 kb on - strand, within Psyr_2352 at 2728.379 kb on - strand, within Psyr_2352 at 2728.379 kb on - strand, within Psyr_2352 at 2728.379 kb on - strand, within Psyr_2352 at 2728.475 kb on + strand at 2728.475 kb on + strand at 2728.495 kb on + strand at 2728.495 kb on + strand at 2728.495 kb on + strand at 2728.496 kb on - strand at 2728.530 kb on + strand at 2728.530 kb on + strand at 2728.531 kb on - strand at 2728.531 kb on - strand at 2728.531 kb on - strand at 2728.565 kb on + strand at 2728.565 kb on + strand at 2728.565 kb on + strand at 2728.565 kb on + strand at 2728.566 kb on - strand at 2728.566 kb on - strand at 2728.566 kb on - strand at 2728.566 kb on - strand at 2728.566 kb on - strand at 2728.570 kb on + strand at 2728.571 kb on - strand at 2728.571 kb on - strand at 2728.571 kb on - strand at 2728.574 kb on + strand at 2728.575 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Matrine 10 mM remove 2,726,126 + +0.5 2,726,126 + -0.7 2,726,168 - -0.7 2,726,168 - -0.2 2,726,173 + -0.7 2,726,173 + +1.3 2,726,174 - -0.6 2,726,180 + -0.7 2,726,180 + +0.5 2,726,180 + +1.2 2,726,180 + -0.4 2,726,181 - -0.1 2,726,181 - +1.2 2,726,181 - -1.6 2,726,181 - +1.0 2,726,181 - -1.7 2,726,181 - -1.1 2,726,210 + +1.3 2,726,211 - -0.6 2,726,236 - -0.4 2,726,242 + +0.4 2,726,268 - +0.2 2,726,288 + +1.0 2,726,426 + +1.5 2,726,426 + +0.3 2,726,426 + +3.4 2,726,431 - +0.8 2,726,451 + -0.1 2,726,451 + +1.0 2,726,451 + +3.8 2,726,451 + -0.5 2,726,451 + -0.1 2,726,451 + -0.5 2,726,451 + -0.9 2,726,452 - +1.5 2,726,452 - +0.4 2,726,452 - -0.7 2,726,452 - +0.1 2,726,452 - +0.5 2,726,452 - -0.2 2,726,452 - -0.2 2,726,519 + -0.4 2,726,594 + Psyr_2350 0.12 -1.0 2,726,652 + Psyr_2350 0.24 -0.4 2,726,652 + Psyr_2350 0.24 +0.3 2,726,652 + Psyr_2350 0.24 +1.0 2,726,653 - Psyr_2350 0.25 +0.3 2,726,653 - Psyr_2350 0.25 -0.5 2,727,026 - +0.2 2,727,026 - -0.6 2,727,039 - +0.0 2,727,055 - +0.8 2,727,064 + +0.0 2,727,101 + +0.7 2,727,219 + Psyr_2351 0.24 -0.6 2,727,219 + Psyr_2351 0.24 -1.0 2,727,220 - Psyr_2351 0.25 -0.7 2,727,222 + Psyr_2351 0.25 +1.6 2,727,222 + Psyr_2351 0.25 +0.8 2,727,222 + Psyr_2351 0.25 -1.6 2,727,222 + Psyr_2351 0.25 -1.4 2,727,222 + Psyr_2351 0.25 -0.9 2,727,223 - Psyr_2351 0.25 -0.3 2,727,223 - Psyr_2351 0.25 -0.4 2,727,259 - Psyr_2351 0.31 -0.2 2,727,631 + -1.1 2,727,631 + -0.6 2,727,632 - -1.4 2,727,632 - -1.3 2,727,632 - -0.8 2,727,702 + -1.3 2,727,743 + +0.3 2,727,926 + +0.1 2,727,926 + +1.1 2,727,949 + -0.9 2,727,950 - -1.0 2,728,003 + Psyr_2352 0.10 +0.8 2,728,075 + Psyr_2352 0.24 -0.1 2,728,075 + Psyr_2352 0.24 -0.3 2,728,075 + Psyr_2352 0.24 +0.2 2,728,075 + Psyr_2352 0.24 +1.2 2,728,076 - Psyr_2352 0.24 -1.1 2,728,076 - Psyr_2352 0.24 -0.2 2,728,225 + Psyr_2352 0.52 -0.5 2,728,225 + Psyr_2352 0.52 -0.5 2,728,378 + Psyr_2352 0.81 +0.7 2,728,378 + Psyr_2352 0.81 +2.2 2,728,378 + Psyr_2352 0.81 -0.1 2,728,378 + Psyr_2352 0.81 -0.4 2,728,379 - Psyr_2352 0.82 +1.2 2,728,379 - Psyr_2352 0.82 +0.0 2,728,379 - Psyr_2352 0.82 -1.1 2,728,379 - Psyr_2352 0.82 +1.2 2,728,475 + +0.7 2,728,475 + +0.6 2,728,495 + -0.2 2,728,495 + +0.9 2,728,495 + -0.7 2,728,496 - -0.5 2,728,530 + -0.5 2,728,530 + -0.8 2,728,531 - -0.4 2,728,531 - -0.4 2,728,531 - -0.8 2,728,565 + +0.3 2,728,565 + +0.7 2,728,565 + +0.8 2,728,565 + -0.1 2,728,566 - -0.5 2,728,566 - +0.1 2,728,566 - +1.7 2,728,566 - -0.4 2,728,566 - +2.2 2,728,570 + +0.6 2,728,571 - +0.3 2,728,571 - -0.2 2,728,571 - +3.2 2,728,574 + +0.4 2,728,575 - -0.6
Or see this region's nucleotide sequence