Experiment: KB with Matrine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2283 and Psyr_2284 are separated by 138 nucleotides Psyr_2284 and Psyr_2285 are separated by 174 nucleotides Psyr_2285 and Psyr_2286 overlap by 7 nucleotides Psyr_2286 and Psyr_2287 are separated by 504 nucleotides
Psyr_2283: Psyr_2283 - Secretion protein HlyD, at 2,653,659 to 2,654,819
_2283
Psyr_2284: Psyr_2284 - transcriptional regulator, LacI family, at 2,654,958 to 2,655,548
_2284
Psyr_2285: Psyr_2285 - Twin-arginine translocation pathway signal, at 2,655,723 to 2,656,328
_2285
Psyr_2286: Psyr_2286 - Protein of unknown function DUF461, at 2,656,322 to 2,656,816
_2286
Psyr_2287: Psyr_2287 - outer membrane transport energization protein TonB, at 2,657,321 to 2,658,154
_2287
Position (kb)
2655
2656
2657 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2654.781 kb on + strand at 2654.781 kb on + strand at 2654.813 kb on + strand at 2654.813 kb on + strand at 2654.814 kb on - strand at 2654.849 kb on - strand at 2654.924 kb on - strand at 2654.926 kb on - strand at 2654.976 kb on - strand at 2655.004 kb on + strand at 2655.004 kb on + strand at 2655.004 kb on + strand at 2655.005 kb on - strand at 2655.578 kb on + strand at 2655.579 kb on - strand at 2655.579 kb on - strand at 2655.662 kb on + strand at 2655.663 kb on - strand at 2656.201 kb on - strand, within Psyr_2285 at 2656.201 kb on - strand, within Psyr_2285 at 2656.201 kb on - strand, within Psyr_2285 at 2656.227 kb on + strand, within Psyr_2285 at 2656.228 kb on - strand, within Psyr_2285 at 2656.321 kb on + strand at 2656.321 kb on + strand at 2656.321 kb on + strand at 2656.321 kb on + strand at 2656.322 kb on - strand at 2656.850 kb on - strand at 2656.861 kb on - strand at 2656.985 kb on + strand at 2656.985 kb on + strand at 2656.986 kb on - strand at 2656.986 kb on - strand at 2657.108 kb on - strand at 2657.172 kb on - strand at 2657.317 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Matrine 10 mM remove 2,654,781 + -0.1 2,654,781 + -0.3 2,654,813 + +0.0 2,654,813 + -0.6 2,654,814 - -0.8 2,654,849 - +1.6 2,654,924 - +0.2 2,654,926 - +0.9 2,654,976 - +2.3 2,655,004 + -1.0 2,655,004 + +0.1 2,655,004 + -0.4 2,655,005 - -0.4 2,655,578 + +0.6 2,655,579 - +1.8 2,655,579 - -0.6 2,655,662 + -0.5 2,655,663 - -0.4 2,656,201 - Psyr_2285 0.79 -1.8 2,656,201 - Psyr_2285 0.79 -0.8 2,656,201 - Psyr_2285 0.79 -2.3 2,656,227 + Psyr_2285 0.83 -2.3 2,656,228 - Psyr_2285 0.83 -0.3 2,656,321 + +0.6 2,656,321 + -0.4 2,656,321 + -0.6 2,656,321 + +1.3 2,656,322 - -0.4 2,656,850 - -0.2 2,656,861 - -0.1 2,656,985 + -1.4 2,656,985 + -1.4 2,656,986 - -0.5 2,656,986 - -0.8 2,657,108 - -0.4 2,657,172 - +1.6 2,657,317 - -0.5
Or see this region's nucleotide sequence