Experiment: KB with Matrine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1862 and Psyr_1863 are separated by 155 nucleotides Psyr_1863 and Psyr_1864 are separated by 200 nucleotides Psyr_1864 and Psyr_1865 are separated by 263 nucleotides
Psyr_1862: Psyr_1862 - Zinc transporter ZIP, at 2,138,075 to 2,139,001
_1862
Psyr_1863: Psyr_1863 - conserved hypothetical protein, at 2,139,157 to 2,139,348
_1863
Psyr_1864: Psyr_1864 - spermidine synthase, at 2,139,549 to 2,140,409
_1864
Psyr_1865: Psyr_1865 - Sulfatase, at 2,140,673 to 2,142,760
_1865
Position (kb)
2139
2140
2141 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2138.859 kb on + strand, within Psyr_1862 at 2138.859 kb on + strand, within Psyr_1862 at 2138.859 kb on + strand, within Psyr_1862 at 2138.859 kb on + strand, within Psyr_1862 at 2138.859 kb on + strand, within Psyr_1862 at 2138.860 kb on - strand, within Psyr_1862 at 2138.860 kb on - strand, within Psyr_1862 at 2138.860 kb on - strand, within Psyr_1862 at 2138.860 kb on - strand, within Psyr_1862 at 2138.930 kb on + strand at 2139.011 kb on + strand at 2139.020 kb on - strand at 2139.048 kb on + strand at 2139.048 kb on + strand at 2139.049 kb on - strand at 2139.050 kb on + strand at 2139.051 kb on - strand at 2139.051 kb on - strand at 2139.080 kb on + strand at 2139.080 kb on + strand at 2139.080 kb on + strand at 2139.080 kb on + strand at 2139.080 kb on + strand at 2139.080 kb on + strand at 2139.081 kb on - strand at 2139.081 kb on - strand at 2139.081 kb on - strand at 2139.081 kb on - strand at 2139.082 kb on + strand at 2139.082 kb on + strand at 2139.082 kb on + strand at 2139.082 kb on + strand at 2139.082 kb on + strand at 2139.083 kb on - strand at 2139.083 kb on - strand at 2139.083 kb on - strand at 2139.083 kb on - strand at 2139.124 kb on - strand at 2139.124 kb on - strand at 2139.165 kb on + strand at 2139.165 kb on + strand at 2139.165 kb on + strand at 2139.165 kb on + strand at 2139.166 kb on - strand at 2139.166 kb on - strand at 2139.166 kb on - strand at 2139.166 kb on - strand at 2139.338 kb on + strand at 2139.338 kb on + strand at 2139.338 kb on + strand at 2139.338 kb on + strand at 2139.338 kb on + strand at 2139.338 kb on + strand at 2139.338 kb on + strand at 2139.339 kb on - strand at 2139.339 kb on - strand at 2139.339 kb on - strand at 2139.339 kb on - strand at 2139.339 kb on - strand at 2139.339 kb on - strand at 2139.349 kb on + strand at 2139.349 kb on + strand at 2139.349 kb on + strand at 2139.349 kb on + strand at 2139.349 kb on + strand at 2139.349 kb on + strand at 2139.349 kb on + strand at 2139.350 kb on - strand at 2139.358 kb on - strand at 2139.382 kb on - strand at 2139.453 kb on + strand at 2139.454 kb on - strand at 2139.483 kb on + strand at 2139.483 kb on + strand at 2139.484 kb on - strand at 2139.573 kb on + strand at 2139.573 kb on + strand at 2139.573 kb on + strand at 2139.574 kb on - strand at 2139.574 kb on - strand at 2139.622 kb on + strand at 2139.622 kb on + strand at 2139.623 kb on - strand at 2139.626 kb on + strand at 2139.626 kb on + strand at 2139.627 kb on - strand at 2139.726 kb on + strand, within Psyr_1864 at 2139.726 kb on + strand, within Psyr_1864 at 2139.848 kb on + strand, within Psyr_1864 at 2139.848 kb on + strand, within Psyr_1864 at 2139.848 kb on + strand, within Psyr_1864 at 2139.848 kb on + strand, within Psyr_1864 at 2139.849 kb on - strand, within Psyr_1864 at 2139.897 kb on + strand, within Psyr_1864 at 2140.195 kb on - strand, within Psyr_1864 at 2140.215 kb on + strand, within Psyr_1864 at 2140.215 kb on + strand, within Psyr_1864 at 2140.215 kb on + strand, within Psyr_1864 at 2140.216 kb on - strand, within Psyr_1864 at 2140.216 kb on - strand, within Psyr_1864 at 2140.216 kb on - strand, within Psyr_1864 at 2140.327 kb on - strand at 2140.329 kb on + strand at 2140.330 kb on - strand at 2140.371 kb on + strand at 2140.371 kb on + strand at 2140.371 kb on + strand at 2140.372 kb on - strand at 2140.372 kb on - strand at 2140.372 kb on - strand at 2140.464 kb on + strand at 2140.465 kb on - strand at 2140.465 kb on - strand at 2140.466 kb on + strand at 2140.467 kb on - strand at 2140.467 kb on - strand at 2140.474 kb on + strand at 2140.580 kb on + strand at 2140.580 kb on + strand at 2140.580 kb on + strand at 2140.581 kb on - strand at 2140.581 kb on - strand at 2140.581 kb on - strand at 2140.583 kb on + strand at 2140.584 kb on - strand at 2140.644 kb on - strand at 2140.644 kb on - strand at 2140.745 kb on + strand at 2140.745 kb on + strand at 2140.746 kb on - strand at 2140.746 kb on - strand at 2140.781 kb on + strand at 2140.781 kb on + strand at 2140.781 kb on + strand at 2140.781 kb on + strand at 2140.782 kb on - strand at 2140.782 kb on - strand at 2140.782 kb on - strand at 2140.782 kb on - strand at 2140.811 kb on + strand at 2140.811 kb on + strand at 2140.812 kb on - strand at 2140.812 kb on - strand at 2140.812 kb on - strand at 2140.901 kb on + strand, within Psyr_1865 at 2140.901 kb on + strand, within Psyr_1865 at 2140.901 kb on + strand, within Psyr_1865 at 2140.901 kb on + strand, within Psyr_1865 at 2140.901 kb on + strand, within Psyr_1865 at 2140.901 kb on + strand, within Psyr_1865 at 2140.901 kb on + strand, within Psyr_1865 at 2140.901 kb on + strand, within Psyr_1865 at 2140.901 kb on + strand, within Psyr_1865 at 2140.902 kb on - strand, within Psyr_1865 at 2140.902 kb on - strand, within Psyr_1865 at 2140.902 kb on - strand, within Psyr_1865 at 2140.902 kb on - strand, within Psyr_1865 at 2140.902 kb on - strand, within Psyr_1865 at 2140.902 kb on - strand, within Psyr_1865 at 2140.902 kb on - strand, within Psyr_1865 at 2140.902 kb on - strand, within Psyr_1865 at 2140.902 kb on - strand, within Psyr_1865 at 2140.902 kb on - strand, within Psyr_1865 at 2140.902 kb on - strand, within Psyr_1865 at 2141.031 kb on - strand, within Psyr_1865 at 2141.031 kb on - strand, within Psyr_1865 at 2141.072 kb on + strand, within Psyr_1865 at 2141.104 kb on + strand, within Psyr_1865 at 2141.105 kb on - strand, within Psyr_1865 at 2141.277 kb on + strand, within Psyr_1865 at 2141.360 kb on + strand, within Psyr_1865 at 2141.361 kb on - strand, within Psyr_1865
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Matrine 10 mM remove 2,138,859 + Psyr_1862 0.85 -0.6 2,138,859 + Psyr_1862 0.85 +0.7 2,138,859 + Psyr_1862 0.85 -0.1 2,138,859 + Psyr_1862 0.85 +0.2 2,138,859 + Psyr_1862 0.85 -0.6 2,138,860 - Psyr_1862 0.85 -0.2 2,138,860 - Psyr_1862 0.85 -0.2 2,138,860 - Psyr_1862 0.85 -0.1 2,138,860 - Psyr_1862 0.85 +0.8 2,138,930 + -1.1 2,139,011 + +0.7 2,139,020 - +0.5 2,139,048 + +0.7 2,139,048 + +2.2 2,139,049 - +1.0 2,139,050 + -0.0 2,139,051 - -1.4 2,139,051 - +0.7 2,139,080 + -1.8 2,139,080 + +0.2 2,139,080 + +1.2 2,139,080 + +2.7 2,139,080 + +1.2 2,139,080 + +0.3 2,139,081 - -0.3 2,139,081 - +0.4 2,139,081 - -0.8 2,139,081 - -0.6 2,139,082 + +0.2 2,139,082 + +0.8 2,139,082 + +0.6 2,139,082 + +0.1 2,139,082 + +0.2 2,139,083 - -0.9 2,139,083 - +0.2 2,139,083 - -0.4 2,139,083 - -0.9 2,139,124 - -0.8 2,139,124 - +0.0 2,139,165 + +0.2 2,139,165 + -0.5 2,139,165 + -0.4 2,139,165 + -0.2 2,139,166 - -0.3 2,139,166 - +0.3 2,139,166 - +0.2 2,139,166 - -0.1 2,139,338 + +0.5 2,139,338 + -0.4 2,139,338 + +0.4 2,139,338 + +0.4 2,139,338 + +0.2 2,139,338 + +1.4 2,139,338 + -0.6 2,139,339 - -0.9 2,139,339 - -1.2 2,139,339 - +1.2 2,139,339 - +0.6 2,139,339 - +0.2 2,139,339 - +0.0 2,139,349 + -0.2 2,139,349 + -0.8 2,139,349 + -0.3 2,139,349 + -0.5 2,139,349 + +1.2 2,139,349 + -0.3 2,139,349 + -0.2 2,139,350 - -0.4 2,139,358 - +0.3 2,139,382 - -0.5 2,139,453 + +0.7 2,139,454 - -0.3 2,139,483 + -0.7 2,139,483 + -1.6 2,139,484 - -1.2 2,139,573 + -0.3 2,139,573 + -1.7 2,139,573 + -1.4 2,139,574 - -2.5 2,139,574 - -0.4 2,139,622 + -0.1 2,139,622 + -0.9 2,139,623 - -0.7 2,139,626 + +0.0 2,139,626 + +0.7 2,139,627 - -0.1 2,139,726 + Psyr_1864 0.21 -0.8 2,139,726 + Psyr_1864 0.21 -0.8 2,139,848 + Psyr_1864 0.35 +3.0 2,139,848 + Psyr_1864 0.35 -0.4 2,139,848 + Psyr_1864 0.35 -0.1 2,139,848 + Psyr_1864 0.35 -2.5 2,139,849 - Psyr_1864 0.35 -1.8 2,139,897 + Psyr_1864 0.40 -1.2 2,140,195 - Psyr_1864 0.75 -1.3 2,140,215 + Psyr_1864 0.77 -0.1 2,140,215 + Psyr_1864 0.77 -0.4 2,140,215 + Psyr_1864 0.77 +0.3 2,140,216 - Psyr_1864 0.77 +0.2 2,140,216 - Psyr_1864 0.77 +3.2 2,140,216 - Psyr_1864 0.77 +1.2 2,140,327 - +0.9 2,140,329 + +1.5 2,140,330 - -2.7 2,140,371 + +0.6 2,140,371 + -1.7 2,140,371 + -1.5 2,140,372 - +0.8 2,140,372 - +0.6 2,140,372 - -0.3 2,140,464 + -0.7 2,140,465 - +0.2 2,140,465 - +0.3 2,140,466 + +0.9 2,140,467 - -1.7 2,140,467 - -0.1 2,140,474 + -1.3 2,140,580 + +1.0 2,140,580 + +1.5 2,140,580 + +0.2 2,140,581 - +0.4 2,140,581 - -0.8 2,140,581 - -1.7 2,140,583 + -0.7 2,140,584 - +0.6 2,140,644 - -0.8 2,140,644 - +0.6 2,140,745 + -0.1 2,140,745 + -1.7 2,140,746 - +1.2 2,140,746 - -0.6 2,140,781 + -0.3 2,140,781 + +0.6 2,140,781 + +0.7 2,140,781 + -0.1 2,140,782 - +0.9 2,140,782 - -0.1 2,140,782 - -0.8 2,140,782 - +0.5 2,140,811 + -0.2 2,140,811 + +0.6 2,140,812 - +0.7 2,140,812 - +0.2 2,140,812 - +1.2 2,140,901 + Psyr_1865 0.11 +0.2 2,140,901 + Psyr_1865 0.11 +0.9 2,140,901 + Psyr_1865 0.11 +0.2 2,140,901 + Psyr_1865 0.11 -0.5 2,140,901 + Psyr_1865 0.11 +0.4 2,140,901 + Psyr_1865 0.11 +0.4 2,140,901 + Psyr_1865 0.11 +0.2 2,140,901 + Psyr_1865 0.11 -1.3 2,140,901 + Psyr_1865 0.11 -0.1 2,140,902 - Psyr_1865 0.11 +0.6 2,140,902 - Psyr_1865 0.11 -1.1 2,140,902 - Psyr_1865 0.11 +0.9 2,140,902 - Psyr_1865 0.11 -0.7 2,140,902 - Psyr_1865 0.11 -0.6 2,140,902 - Psyr_1865 0.11 +2.2 2,140,902 - Psyr_1865 0.11 -0.3 2,140,902 - Psyr_1865 0.11 +0.2 2,140,902 - Psyr_1865 0.11 -0.7 2,140,902 - Psyr_1865 0.11 -0.0 2,140,902 - Psyr_1865 0.11 -2.4 2,141,031 - Psyr_1865 0.17 +0.1 2,141,031 - Psyr_1865 0.17 -0.1 2,141,072 + Psyr_1865 0.19 +0.3 2,141,104 + Psyr_1865 0.21 -1.5 2,141,105 - Psyr_1865 0.21 +0.2 2,141,277 + Psyr_1865 0.29 +0.1 2,141,360 + Psyr_1865 0.33 -1.0 2,141,361 - Psyr_1865 0.33 +1.0
Or see this region's nucleotide sequence