Experiment: KB with Matrine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1415 and Psyr_1416 are separated by 52 nucleotides Psyr_1416 and Psyr_1417 are separated by 78 nucleotides Psyr_1417 and Psyr_1418 are separated by 171 nucleotides Psyr_1418 and Psyr_1419 are separated by 1 nucleotides
Psyr_1415: Psyr_1415 - TolB, N-terminal:TolB, N-terminal, at 1,600,063 to 1,601,364
_1415
Psyr_1416: Psyr_1416 - OmpA/MotB, at 1,601,417 to 1,601,920
_1416
Psyr_1417: Psyr_1417 - TPR repeat protein, at 1,601,999 to 1,602,745
_1417
Psyr_1418: Psyr_1418 - Radical SAM, at 1,602,917 to 1,603,564
_1418
Psyr_1419: Psyr_1419 - preQ(0) biosynthesis protein QueC, at 1,603,566 to 1,604,255
_1419
Position (kb)
1601
1602
1603 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1601.353 kb on + strand at 1601.404 kb on + strand at 1601.404 kb on + strand at 1601.914 kb on + strand at 1601.918 kb on + strand at 1601.918 kb on + strand at 1602.241 kb on + strand, within Psyr_1417 at 1602.241 kb on + strand, within Psyr_1417 at 1602.241 kb on + strand, within Psyr_1417 at 1602.241 kb on + strand, within Psyr_1417 at 1602.242 kb on - strand, within Psyr_1417 at 1602.242 kb on - strand, within Psyr_1417 at 1602.242 kb on - strand, within Psyr_1417 at 1602.242 kb on - strand, within Psyr_1417 at 1602.242 kb on - strand, within Psyr_1417 at 1602.242 kb on - strand, within Psyr_1417 at 1602.242 kb on - strand, within Psyr_1417 at 1602.242 kb on - strand, within Psyr_1417 at 1602.242 kb on - strand, within Psyr_1417 at 1602.242 kb on - strand, within Psyr_1417 at 1602.242 kb on - strand, within Psyr_1417 at 1602.242 kb on - strand, within Psyr_1417 at 1602.242 kb on - strand, within Psyr_1417 at 1602.274 kb on + strand, within Psyr_1417 at 1602.275 kb on - strand, within Psyr_1417 at 1602.389 kb on + strand, within Psyr_1417 at 1602.390 kb on - strand, within Psyr_1417 at 1602.470 kb on + strand, within Psyr_1417 at 1602.471 kb on - strand, within Psyr_1417 at 1602.486 kb on - strand, within Psyr_1417 at 1602.614 kb on + strand, within Psyr_1417 at 1602.614 kb on + strand, within Psyr_1417 at 1602.615 kb on - strand, within Psyr_1417 at 1602.669 kb on + strand, within Psyr_1417 at 1602.669 kb on + strand, within Psyr_1417 at 1602.670 kb on - strand, within Psyr_1417 at 1602.778 kb on - strand at 1602.828 kb on + strand at 1602.894 kb on + strand at 1602.894 kb on + strand at 1602.894 kb on + strand at 1602.894 kb on + strand at 1602.930 kb on + strand at 1602.930 kb on + strand at 1602.993 kb on + strand, within Psyr_1418 at 1603.118 kb on + strand, within Psyr_1418 at 1603.118 kb on + strand, within Psyr_1418 at 1603.119 kb on - strand, within Psyr_1418 at 1603.203 kb on + strand, within Psyr_1418 at 1603.203 kb on + strand, within Psyr_1418 at 1603.204 kb on - strand, within Psyr_1418 at 1603.310 kb on + strand, within Psyr_1418 at 1603.377 kb on - strand, within Psyr_1418 at 1603.407 kb on - strand, within Psyr_1418 at 1603.565 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Matrine 10 mM remove 1,601,353 + -0.4 1,601,404 + -1.9 1,601,404 + -0.1 1,601,914 + -0.4 1,601,918 + -0.0 1,601,918 + -0.4 1,602,241 + Psyr_1417 0.32 -0.9 1,602,241 + Psyr_1417 0.32 -1.4 1,602,241 + Psyr_1417 0.32 +2.2 1,602,241 + Psyr_1417 0.32 +0.8 1,602,242 - Psyr_1417 0.33 -2.2 1,602,242 - Psyr_1417 0.33 +0.4 1,602,242 - Psyr_1417 0.33 -0.8 1,602,242 - Psyr_1417 0.33 -1.4 1,602,242 - Psyr_1417 0.33 -0.4 1,602,242 - Psyr_1417 0.33 -2.1 1,602,242 - Psyr_1417 0.33 -1.7 1,602,242 - Psyr_1417 0.33 -1.8 1,602,242 - Psyr_1417 0.33 +0.6 1,602,242 - Psyr_1417 0.33 -1.4 1,602,242 - Psyr_1417 0.33 -0.8 1,602,242 - Psyr_1417 0.33 -1.1 1,602,242 - Psyr_1417 0.33 -2.6 1,602,274 + Psyr_1417 0.37 -0.6 1,602,275 - Psyr_1417 0.37 -2.4 1,602,389 + Psyr_1417 0.52 +1.7 1,602,390 - Psyr_1417 0.52 -1.0 1,602,470 + Psyr_1417 0.63 -0.5 1,602,471 - Psyr_1417 0.63 -1.6 1,602,486 - Psyr_1417 0.65 +0.6 1,602,614 + Psyr_1417 0.82 -0.8 1,602,614 + Psyr_1417 0.82 -0.1 1,602,615 - Psyr_1417 0.82 -1.4 1,602,669 + Psyr_1417 0.90 +1.0 1,602,669 + Psyr_1417 0.90 -0.2 1,602,670 - Psyr_1417 0.90 +0.4 1,602,778 - +1.1 1,602,828 + -0.5 1,602,894 + -0.6 1,602,894 + +1.5 1,602,894 + -0.4 1,602,894 + +0.0 1,602,930 + +0.2 1,602,930 + -0.4 1,602,993 + Psyr_1418 0.12 -0.9 1,603,118 + Psyr_1418 0.31 -1.6 1,603,118 + Psyr_1418 0.31 -1.3 1,603,119 - Psyr_1418 0.31 -1.6 1,603,203 + Psyr_1418 0.44 +1.8 1,603,203 + Psyr_1418 0.44 -1.5 1,603,204 - Psyr_1418 0.44 -1.5 1,603,310 + Psyr_1418 0.61 -0.8 1,603,377 - Psyr_1418 0.71 -0.8 1,603,407 - Psyr_1418 0.76 -1.1 1,603,565 + -0.2
Or see this region's nucleotide sequence