Experiment: KB with Matrine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1226 and Psyr_RNA20 are separated by 202 nucleotides Psyr_RNA20 and Psyr_1227 are separated by 117 nucleotides Psyr_1227 and Psyr_1228 overlap by 4 nucleotides
Psyr_1226: Psyr_1226 - hypothetical protein, at 1,384,596 to 1,384,949
_1226
Psyr_RNA20: Psyr_RNA20 - tRNA-Leu, at 1,385,152 to 1,385,236
_RNA20
Psyr_1227: Psyr_1227 - Queuosine biosynthesis protein, at 1,385,354 to 1,386,418
_1227
Psyr_1228: Psyr_1228 - tRNA-guanine transglycosylase, at 1,386,415 to 1,387,548
_1228
Position (kb) 1385
1386
1387 Strain fitness (log2 ratio) -2
-1
0
1 at 1384.394 kb on + strand at 1384.395 kb on - strand at 1384.399 kb on + strand at 1384.400 kb on - strand at 1384.429 kb on + strand at 1384.430 kb on - strand at 1384.430 kb on - strand at 1384.430 kb on - strand at 1384.457 kb on + strand at 1384.482 kb on + strand at 1384.482 kb on + strand at 1384.601 kb on - strand at 1384.601 kb on - strand at 1384.601 kb on - strand at 1384.753 kb on - strand, within Psyr_1226 at 1384.777 kb on - strand, within Psyr_1226 at 1384.890 kb on + strand, within Psyr_1226 at 1384.897 kb on + strand, within Psyr_1226 at 1384.898 kb on - strand, within Psyr_1226 at 1385.032 kb on + strand at 1385.032 kb on + strand at 1385.032 kb on + strand at 1385.033 kb on - strand at 1385.033 kb on - strand at 1385.033 kb on - strand at 1385.041 kb on + strand at 1385.042 kb on - strand at 1385.042 kb on - strand at 1385.052 kb on + strand at 1385.052 kb on + strand at 1385.052 kb on + strand at 1385.053 kb on - strand at 1385.053 kb on - strand at 1385.053 kb on - strand at 1385.148 kb on + strand at 1385.149 kb on - strand at 1385.149 kb on - strand at 1385.149 kb on - strand at 1385.149 kb on - strand at 1385.149 kb on - strand at 1385.149 kb on - strand at 1385.149 kb on - strand at 1385.149 kb on - strand at 1385.149 kb on - strand at 1385.149 kb on - strand at 1385.149 kb on - strand at 1385.149 kb on - strand at 1385.149 kb on - strand at 1385.149 kb on - strand at 1385.287 kb on + strand at 1385.303 kb on + strand at 1385.795 kb on + strand, within Psyr_1227 at 1385.795 kb on + strand, within Psyr_1227 at 1385.795 kb on + strand, within Psyr_1227 at 1385.795 kb on + strand, within Psyr_1227 at 1385.797 kb on + strand, within Psyr_1227 at 1385.797 kb on + strand, within Psyr_1227 at 1385.799 kb on + strand, within Psyr_1227 at 1385.799 kb on + strand, within Psyr_1227 at 1385.799 kb on + strand, within Psyr_1227 at 1386.535 kb on + strand, within Psyr_1228 at 1387.416 kb on + strand, within Psyr_1228
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Matrine 10 mM remove 1,384,394 + +0.8 1,384,395 - +0.5 1,384,399 + -0.9 1,384,400 - -0.4 1,384,429 + +0.2 1,384,430 - +1.5 1,384,430 - -1.0 1,384,430 - -1.1 1,384,457 + +0.9 1,384,482 + -1.3 1,384,482 + -1.0 1,384,601 - +0.4 1,384,601 - +0.2 1,384,601 - -1.0 1,384,753 - Psyr_1226 0.44 -0.1 1,384,777 - Psyr_1226 0.51 +0.3 1,384,890 + Psyr_1226 0.83 +0.9 1,384,897 + Psyr_1226 0.85 +0.3 1,384,898 - Psyr_1226 0.85 +0.8 1,385,032 + +1.0 1,385,032 + -0.0 1,385,032 + +1.4 1,385,033 - +0.9 1,385,033 - +0.2 1,385,033 - -1.8 1,385,041 + -1.0 1,385,042 - -0.1 1,385,042 - +0.5 1,385,052 + +0.4 1,385,052 + -1.2 1,385,052 + -0.3 1,385,053 - -0.6 1,385,053 - +0.2 1,385,053 - +0.2 1,385,148 + +0.9 1,385,149 - -1.6 1,385,149 - -1.7 1,385,149 - -1.1 1,385,149 - +0.2 1,385,149 - +0.2 1,385,149 - +0.6 1,385,149 - +0.9 1,385,149 - +0.4 1,385,149 - -0.4 1,385,149 - -0.1 1,385,149 - +1.7 1,385,149 - -1.8 1,385,149 - -1.6 1,385,149 - -0.1 1,385,287 + +1.4 1,385,303 + -0.7 1,385,795 + Psyr_1227 0.41 +0.2 1,385,795 + Psyr_1227 0.41 -0.9 1,385,795 + Psyr_1227 0.41 +0.4 1,385,795 + Psyr_1227 0.41 +0.5 1,385,797 + Psyr_1227 0.42 -1.7 1,385,797 + Psyr_1227 0.42 +0.2 1,385,799 + Psyr_1227 0.42 +0.4 1,385,799 + Psyr_1227 0.42 -1.0 1,385,799 + Psyr_1227 0.42 +0.0 1,386,535 + Psyr_1228 0.11 -1.4 1,387,416 + Psyr_1228 0.88 -2.3
Or see this region's nucleotide sequence