Experiment: KB with Matrine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0989 and Psyr_0990 are separated by 169 nucleotides Psyr_0990 and Psyr_0991 are separated by 309 nucleotides
Psyr_0989: Psyr_0989 - Lysine exporter protein (LYSE/YGGA), at 1,119,347 to 1,119,979
_0989
Psyr_0990: Psyr_0990 - AMP-dependent synthetase and ligase, at 1,120,149 to 1,121,831
_0990
Psyr_0991: Psyr_0991 - Protein of unknown function DUF1302, at 1,122,141 to 1,124,078
_0991
Position (kb)
1120
1121
1122 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1119.244 kb on + strand at 1119.244 kb on + strand at 1119.245 kb on - strand at 1119.248 kb on + strand at 1119.248 kb on + strand at 1119.248 kb on + strand at 1119.248 kb on + strand at 1119.248 kb on + strand at 1119.249 kb on - strand at 1119.306 kb on + strand at 1119.306 kb on + strand at 1119.350 kb on + strand at 1119.351 kb on - strand at 1119.351 kb on - strand at 1119.351 kb on - strand at 1119.351 kb on - strand at 1119.591 kb on - strand, within Psyr_0989 at 1119.594 kb on + strand, within Psyr_0989 at 1119.594 kb on + strand, within Psyr_0989 at 1119.594 kb on + strand, within Psyr_0989 at 1119.594 kb on + strand, within Psyr_0989 at 1119.594 kb on + strand, within Psyr_0989 at 1119.594 kb on + strand, within Psyr_0989 at 1119.595 kb on - strand, within Psyr_0989 at 1119.595 kb on - strand, within Psyr_0989 at 1119.595 kb on - strand, within Psyr_0989 at 1119.595 kb on - strand, within Psyr_0989 at 1119.595 kb on - strand, within Psyr_0989 at 1119.595 kb on - strand, within Psyr_0989 at 1119.595 kb on - strand, within Psyr_0989 at 1119.595 kb on - strand, within Psyr_0989 at 1119.595 kb on - strand, within Psyr_0989 at 1119.595 kb on - strand, within Psyr_0989 at 1119.595 kb on - strand, within Psyr_0989 at 1119.599 kb on + strand, within Psyr_0989 at 1119.599 kb on + strand, within Psyr_0989 at 1119.599 kb on + strand, within Psyr_0989 at 1119.600 kb on - strand, within Psyr_0989 at 1119.600 kb on - strand, within Psyr_0989 at 1119.600 kb on - strand, within Psyr_0989 at 1119.600 kb on - strand, within Psyr_0989 at 1119.600 kb on - strand, within Psyr_0989 at 1119.600 kb on - strand, within Psyr_0989 at 1119.600 kb on - strand, within Psyr_0989 at 1119.695 kb on + strand at 1119.695 kb on + strand, within Psyr_0989 at 1119.695 kb on + strand, within Psyr_0989 at 1119.696 kb on - strand, within Psyr_0989 at 1119.696 kb on - strand, within Psyr_0989 at 1119.696 kb on - strand, within Psyr_0989 at 1119.696 kb on - strand, within Psyr_0989 at 1119.696 kb on - strand, within Psyr_0989 at 1119.815 kb on + strand, within Psyr_0989 at 1120.109 kb on + strand at 1120.110 kb on - strand at 1120.110 kb on - strand at 1120.110 kb on - strand at 1120.148 kb on + strand at 1120.148 kb on + strand at 1120.148 kb on + strand at 1120.148 kb on + strand at 1120.148 kb on + strand at 1120.149 kb on - strand at 1120.149 kb on - strand at 1120.149 kb on - strand at 1120.149 kb on - strand at 1120.149 kb on - strand at 1120.149 kb on - strand at 1120.149 kb on - strand at 1120.149 kb on - strand at 1120.260 kb on + strand at 1120.260 kb on + strand at 1120.260 kb on + strand at 1120.261 kb on - strand at 1120.278 kb on + strand at 1120.284 kb on + strand at 1120.285 kb on - strand at 1120.306 kb on + strand at 1120.306 kb on + strand at 1120.307 kb on - strand at 1120.324 kb on + strand, within Psyr_0990 at 1120.324 kb on + strand, within Psyr_0990 at 1120.324 kb on + strand, within Psyr_0990 at 1120.389 kb on + strand, within Psyr_0990 at 1120.533 kb on + strand, within Psyr_0990 at 1120.533 kb on + strand, within Psyr_0990 at 1120.533 kb on + strand, within Psyr_0990 at 1120.534 kb on - strand, within Psyr_0990 at 1120.534 kb on - strand, within Psyr_0990 at 1120.621 kb on + strand, within Psyr_0990 at 1120.621 kb on + strand, within Psyr_0990 at 1120.622 kb on - strand, within Psyr_0990 at 1120.622 kb on - strand, within Psyr_0990 at 1120.622 kb on - strand, within Psyr_0990 at 1120.632 kb on + strand, within Psyr_0990 at 1120.633 kb on - strand, within Psyr_0990 at 1120.633 kb on - strand, within Psyr_0990 at 1120.665 kb on + strand, within Psyr_0990 at 1120.666 kb on - strand, within Psyr_0990 at 1120.666 kb on - strand, within Psyr_0990 at 1120.710 kb on + strand, within Psyr_0990 at 1120.710 kb on + strand, within Psyr_0990 at 1120.711 kb on - strand, within Psyr_0990 at 1120.711 kb on - strand, within Psyr_0990 at 1120.711 kb on - strand, within Psyr_0990 at 1120.711 kb on - strand, within Psyr_0990 at 1120.746 kb on + strand, within Psyr_0990 at 1120.746 kb on + strand, within Psyr_0990 at 1120.746 kb on + strand, within Psyr_0990 at 1120.746 kb on + strand, within Psyr_0990 at 1120.746 kb on + strand, within Psyr_0990 at 1120.746 kb on + strand, within Psyr_0990 at 1120.747 kb on - strand, within Psyr_0990 at 1120.747 kb on - strand, within Psyr_0990 at 1120.842 kb on + strand, within Psyr_0990 at 1120.842 kb on + strand, within Psyr_0990 at 1120.842 kb on + strand, within Psyr_0990 at 1120.842 kb on + strand, within Psyr_0990 at 1120.842 kb on + strand, within Psyr_0990 at 1120.842 kb on + strand, within Psyr_0990 at 1120.843 kb on - strand, within Psyr_0990 at 1120.843 kb on - strand, within Psyr_0990 at 1120.843 kb on - strand, within Psyr_0990 at 1120.843 kb on - strand, within Psyr_0990 at 1120.843 kb on - strand, within Psyr_0990 at 1120.843 kb on - strand, within Psyr_0990 at 1120.843 kb on - strand, within Psyr_0990 at 1120.843 kb on - strand, within Psyr_0990 at 1120.923 kb on + strand, within Psyr_0990 at 1121.170 kb on + strand, within Psyr_0990 at 1121.230 kb on - strand, within Psyr_0990 at 1121.361 kb on + strand, within Psyr_0990 at 1121.361 kb on + strand, within Psyr_0990 at 1121.361 kb on + strand, within Psyr_0990 at 1121.362 kb on - strand, within Psyr_0990 at 1121.362 kb on - strand, within Psyr_0990 at 1121.362 kb on - strand, within Psyr_0990 at 1121.693 kb on + strand at 1121.693 kb on + strand at 1121.693 kb on + strand at 1121.693 kb on + strand at 1121.693 kb on + strand at 1121.693 kb on + strand at 1121.694 kb on - strand at 1121.694 kb on - strand at 1121.694 kb on - strand at 1121.694 kb on - strand at 1121.694 kb on - strand at 1121.694 kb on - strand at 1121.694 kb on - strand at 1121.694 kb on - strand at 1121.915 kb on - strand at 1122.007 kb on + strand at 1122.007 kb on + strand at 1122.008 kb on - strand at 1122.008 kb on - strand at 1122.029 kb on + strand at 1122.113 kb on + strand at 1122.119 kb on + strand at 1122.119 kb on + strand at 1122.120 kb on - strand at 1122.120 kb on - strand at 1122.441 kb on + strand, within Psyr_0991 at 1122.442 kb on - strand, within Psyr_0991 at 1122.442 kb on - strand, within Psyr_0991 at 1122.474 kb on + strand, within Psyr_0991 at 1122.475 kb on - strand, within Psyr_0991 at 1122.475 kb on - strand, within Psyr_0991 at 1122.475 kb on - strand, within Psyr_0991 at 1122.475 kb on - strand, within Psyr_0991 at 1122.588 kb on + strand, within Psyr_0991 at 1122.589 kb on - strand, within Psyr_0991 at 1122.597 kb on + strand, within Psyr_0991 at 1122.597 kb on + strand, within Psyr_0991 at 1122.597 kb on + strand, within Psyr_0991 at 1122.598 kb on - strand, within Psyr_0991 at 1122.598 kb on - strand, within Psyr_0991 at 1122.662 kb on + strand, within Psyr_0991 at 1122.662 kb on + strand, within Psyr_0991 at 1122.663 kb on - strand, within Psyr_0991 at 1122.663 kb on - strand, within Psyr_0991 at 1122.663 kb on - strand, within Psyr_0991 at 1122.663 kb on - strand, within Psyr_0991 at 1122.768 kb on + strand, within Psyr_0991 at 1122.768 kb on + strand, within Psyr_0991 at 1122.768 kb on + strand, within Psyr_0991 at 1122.768 kb on + strand, within Psyr_0991 at 1122.768 kb on + strand, within Psyr_0991 at 1122.769 kb on - strand, within Psyr_0991 at 1122.769 kb on - strand, within Psyr_0991 at 1122.769 kb on - strand, within Psyr_0991 at 1122.813 kb on + strand, within Psyr_0991 at 1122.814 kb on - strand, within Psyr_0991 at 1122.814 kb on - strand, within Psyr_0991
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Matrine 10 mM remove 1,119,244 + +0.4 1,119,244 + +1.5 1,119,245 - +1.9 1,119,248 + +0.4 1,119,248 + +0.1 1,119,248 + +1.4 1,119,248 + +1.6 1,119,248 + -0.4 1,119,249 - +1.0 1,119,306 + +0.6 1,119,306 + +0.3 1,119,350 + +0.2 1,119,351 - -0.7 1,119,351 - -0.2 1,119,351 - -0.3 1,119,351 - +0.6 1,119,591 - Psyr_0989 0.39 -0.6 1,119,594 + Psyr_0989 0.39 +0.5 1,119,594 + Psyr_0989 0.39 +0.4 1,119,594 + Psyr_0989 0.39 +1.1 1,119,594 + Psyr_0989 0.39 +0.3 1,119,594 + Psyr_0989 0.39 +0.1 1,119,594 + Psyr_0989 0.39 +1.0 1,119,595 - Psyr_0989 0.39 -1.1 1,119,595 - Psyr_0989 0.39 +0.1 1,119,595 - Psyr_0989 0.39 +0.1 1,119,595 - Psyr_0989 0.39 +0.1 1,119,595 - Psyr_0989 0.39 -0.6 1,119,595 - Psyr_0989 0.39 +1.2 1,119,595 - Psyr_0989 0.39 +0.6 1,119,595 - Psyr_0989 0.39 -0.5 1,119,595 - Psyr_0989 0.39 -0.8 1,119,595 - Psyr_0989 0.39 -1.1 1,119,595 - Psyr_0989 0.39 +0.7 1,119,599 + Psyr_0989 0.40 -1.6 1,119,599 + Psyr_0989 0.40 +0.1 1,119,599 + Psyr_0989 0.40 -1.3 1,119,600 - Psyr_0989 0.40 -0.4 1,119,600 - Psyr_0989 0.40 +0.1 1,119,600 - Psyr_0989 0.40 -0.3 1,119,600 - Psyr_0989 0.40 -0.3 1,119,600 - Psyr_0989 0.40 +0.4 1,119,600 - Psyr_0989 0.40 -0.8 1,119,600 - Psyr_0989 0.40 -0.3 1,119,695 + +0.2 1,119,695 + Psyr_0989 0.55 -1.6 1,119,695 + Psyr_0989 0.55 +0.0 1,119,696 - Psyr_0989 0.55 +0.8 1,119,696 - Psyr_0989 0.55 -1.1 1,119,696 - Psyr_0989 0.55 -0.8 1,119,696 - Psyr_0989 0.55 -0.4 1,119,696 - Psyr_0989 0.55 -0.5 1,119,815 + Psyr_0989 0.74 +0.7 1,120,109 + +0.7 1,120,110 - -1.2 1,120,110 - -0.5 1,120,110 - -0.1 1,120,148 + -0.2 1,120,148 + -0.2 1,120,148 + +0.2 1,120,148 + +1.1 1,120,148 + +1.4 1,120,149 - +1.2 1,120,149 - -0.3 1,120,149 - +0.1 1,120,149 - -0.1 1,120,149 - -0.6 1,120,149 - -0.3 1,120,149 - +0.2 1,120,149 - +0.1 1,120,260 + -0.6 1,120,260 + -0.8 1,120,260 + -0.1 1,120,261 - +0.2 1,120,278 + +2.6 1,120,284 + -0.4 1,120,285 - -0.8 1,120,306 + +0.1 1,120,306 + +0.1 1,120,307 - -0.3 1,120,324 + Psyr_0990 0.10 +0.2 1,120,324 + Psyr_0990 0.10 +0.2 1,120,324 + Psyr_0990 0.10 -0.2 1,120,389 + Psyr_0990 0.14 +0.1 1,120,533 + Psyr_0990 0.23 +0.5 1,120,533 + Psyr_0990 0.23 -0.8 1,120,533 + Psyr_0990 0.23 +0.6 1,120,534 - Psyr_0990 0.23 +1.2 1,120,534 - Psyr_0990 0.23 -2.1 1,120,621 + Psyr_0990 0.28 +0.2 1,120,621 + Psyr_0990 0.28 +1.9 1,120,622 - Psyr_0990 0.28 -0.6 1,120,622 - Psyr_0990 0.28 +1.6 1,120,622 - Psyr_0990 0.28 -1.0 1,120,632 + Psyr_0990 0.29 +0.7 1,120,633 - Psyr_0990 0.29 -0.2 1,120,633 - Psyr_0990 0.29 +0.1 1,120,665 + Psyr_0990 0.31 -0.3 1,120,666 - Psyr_0990 0.31 +0.6 1,120,666 - Psyr_0990 0.31 +0.7 1,120,710 + Psyr_0990 0.33 -1.0 1,120,710 + Psyr_0990 0.33 -1.8 1,120,711 - Psyr_0990 0.33 -0.2 1,120,711 - Psyr_0990 0.33 +0.9 1,120,711 - Psyr_0990 0.33 +0.5 1,120,711 - Psyr_0990 0.33 +0.7 1,120,746 + Psyr_0990 0.35 +1.0 1,120,746 + Psyr_0990 0.35 +0.0 1,120,746 + Psyr_0990 0.35 -0.0 1,120,746 + Psyr_0990 0.35 -0.1 1,120,746 + Psyr_0990 0.35 +1.2 1,120,746 + Psyr_0990 0.35 +0.5 1,120,747 - Psyr_0990 0.36 +1.2 1,120,747 - Psyr_0990 0.36 +1.0 1,120,842 + Psyr_0990 0.41 -0.7 1,120,842 + Psyr_0990 0.41 +1.0 1,120,842 + Psyr_0990 0.41 +0.6 1,120,842 + Psyr_0990 0.41 +0.6 1,120,842 + Psyr_0990 0.41 -0.2 1,120,842 + Psyr_0990 0.41 -1.4 1,120,843 - Psyr_0990 0.41 -0.1 1,120,843 - Psyr_0990 0.41 -0.2 1,120,843 - Psyr_0990 0.41 -0.5 1,120,843 - Psyr_0990 0.41 +0.6 1,120,843 - Psyr_0990 0.41 +0.4 1,120,843 - Psyr_0990 0.41 -0.7 1,120,843 - Psyr_0990 0.41 +0.4 1,120,843 - Psyr_0990 0.41 -0.4 1,120,923 + Psyr_0990 0.46 -0.3 1,121,170 + Psyr_0990 0.61 +0.6 1,121,230 - Psyr_0990 0.64 +1.3 1,121,361 + Psyr_0990 0.72 +1.3 1,121,361 + Psyr_0990 0.72 +0.5 1,121,361 + Psyr_0990 0.72 -0.1 1,121,362 - Psyr_0990 0.72 -1.0 1,121,362 - Psyr_0990 0.72 +0.8 1,121,362 - Psyr_0990 0.72 -0.4 1,121,693 + +0.2 1,121,693 + +0.2 1,121,693 + -0.1 1,121,693 + +0.5 1,121,693 + -0.8 1,121,693 + -0.4 1,121,694 - -0.5 1,121,694 - +1.2 1,121,694 - +1.2 1,121,694 - -0.6 1,121,694 - -0.6 1,121,694 - +0.1 1,121,694 - -0.1 1,121,694 - +0.6 1,121,915 - -0.3 1,122,007 + -0.8 1,122,007 + -0.5 1,122,008 - +0.3 1,122,008 - +0.2 1,122,029 + +0.4 1,122,113 + +0.1 1,122,119 + -0.1 1,122,119 + +1.2 1,122,120 - -0.0 1,122,120 - +0.8 1,122,441 + Psyr_0991 0.15 -1.2 1,122,442 - Psyr_0991 0.16 -0.9 1,122,442 - Psyr_0991 0.16 -0.8 1,122,474 + Psyr_0991 0.17 +0.6 1,122,475 - Psyr_0991 0.17 -0.3 1,122,475 - Psyr_0991 0.17 -2.3 1,122,475 - Psyr_0991 0.17 +0.1 1,122,475 - Psyr_0991 0.17 -0.4 1,122,588 + Psyr_0991 0.23 -1.1 1,122,589 - Psyr_0991 0.23 -0.8 1,122,597 + Psyr_0991 0.24 +0.9 1,122,597 + Psyr_0991 0.24 +0.9 1,122,597 + Psyr_0991 0.24 +0.2 1,122,598 - Psyr_0991 0.24 +1.0 1,122,598 - Psyr_0991 0.24 -0.4 1,122,662 + Psyr_0991 0.27 -0.1 1,122,662 + Psyr_0991 0.27 -1.0 1,122,663 - Psyr_0991 0.27 -0.6 1,122,663 - Psyr_0991 0.27 +0.4 1,122,663 - Psyr_0991 0.27 +0.6 1,122,663 - Psyr_0991 0.27 -1.0 1,122,768 + Psyr_0991 0.32 -0.0 1,122,768 + Psyr_0991 0.32 +0.2 1,122,768 + Psyr_0991 0.32 -1.0 1,122,768 + Psyr_0991 0.32 -1.4 1,122,768 + Psyr_0991 0.32 -0.8 1,122,769 - Psyr_0991 0.32 -0.0 1,122,769 - Psyr_0991 0.32 +1.4 1,122,769 - Psyr_0991 0.32 -1.8 1,122,813 + Psyr_0991 0.35 +0.6 1,122,814 - Psyr_0991 0.35 -0.7 1,122,814 - Psyr_0991 0.35 +0.7
Or see this region's nucleotide sequence