Experiment: KB with Matrine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0474 and Psyr_0475 are separated by 266 nucleotides Psyr_0475 and Psyr_0476 are separated by 25 nucleotides Psyr_0476 and Psyr_0477 are separated by 21 nucleotides
Psyr_0474: Psyr_0474 - homoserine O-acetyltransferase, at 512,859 to 513,998
_0474
Psyr_0475: Psyr_0475 - Protein of unknown function YGGT, at 514,265 to 514,855
_0475
Psyr_0476: Psyr_0476 - pyrroline-5-carboxylate reductase, at 514,881 to 515,699
_0476
Psyr_0477: Psyr_0477 - Protein of unknown function UPF0001, at 515,721 to 516,407
_0477
Position (kb) 514
515 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 513.298 kb on + strand, within Psyr_0474 at 513.298 kb on + strand, within Psyr_0474 at 513.299 kb on - strand, within Psyr_0474 at 513.299 kb on - strand, within Psyr_0474 at 513.299 kb on - strand, within Psyr_0474 at 513.299 kb on - strand, within Psyr_0474 at 513.723 kb on - strand, within Psyr_0474 at 513.874 kb on + strand, within Psyr_0474 at 513.874 kb on + strand, within Psyr_0474 at 513.875 kb on - strand, within Psyr_0474 at 513.875 kb on - strand, within Psyr_0474 at 513.883 kb on + strand, within Psyr_0474 at 513.883 kb on + strand, within Psyr_0474 at 513.895 kb on + strand at 513.895 kb on + strand at 513.896 kb on - strand at 513.896 kb on - strand at 513.896 kb on - strand at 513.988 kb on - strand at 514.015 kb on + strand at 514.016 kb on - strand at 514.026 kb on + strand at 514.026 kb on + strand at 514.026 kb on + strand at 514.026 kb on + strand at 514.026 kb on + strand at 514.026 kb on + strand at 514.026 kb on + strand at 514.027 kb on - strand at 514.027 kb on - strand at 514.027 kb on - strand at 514.032 kb on + strand at 514.032 kb on + strand at 514.058 kb on - strand at 514.064 kb on + strand at 514.064 kb on + strand at 514.092 kb on + strand at 514.092 kb on + strand at 514.092 kb on + strand at 514.092 kb on + strand at 514.093 kb on - strand at 514.093 kb on - strand at 514.096 kb on + strand at 514.096 kb on + strand at 514.096 kb on + strand at 514.097 kb on - strand at 514.097 kb on - strand at 514.107 kb on + strand at 514.107 kb on + strand at 514.108 kb on - strand at 514.108 kb on - strand at 514.108 kb on - strand at 514.108 kb on - strand at 514.108 kb on - strand at 514.146 kb on + strand at 514.147 kb on - strand at 514.147 kb on - strand at 514.148 kb on + strand at 514.148 kb on + strand at 514.148 kb on + strand at 514.148 kb on + strand at 514.148 kb on + strand at 514.149 kb on - strand at 514.149 kb on - strand at 514.149 kb on - strand at 514.149 kb on - strand at 514.149 kb on - strand at 514.163 kb on + strand at 514.163 kb on + strand at 514.163 kb on + strand at 514.163 kb on + strand at 514.163 kb on + strand at 514.163 kb on + strand at 514.163 kb on + strand at 514.163 kb on + strand at 514.164 kb on - strand at 514.164 kb on - strand at 514.164 kb on - strand at 514.164 kb on - strand at 514.164 kb on - strand at 514.164 kb on - strand at 514.164 kb on - strand at 514.164 kb on - strand at 514.164 kb on - strand at 514.164 kb on - strand at 514.164 kb on - strand at 514.164 kb on - strand at 514.164 kb on - strand at 514.164 kb on - strand at 514.164 kb on - strand at 514.167 kb on + strand at 514.266 kb on + strand at 514.266 kb on + strand at 514.266 kb on + strand at 514.266 kb on + strand at 514.266 kb on + strand at 514.266 kb on + strand at 514.266 kb on + strand at 514.266 kb on + strand at 514.266 kb on + strand at 514.266 kb on + strand at 514.266 kb on + strand at 514.266 kb on + strand at 514.266 kb on + strand at 514.267 kb on - strand at 514.267 kb on - strand at 514.267 kb on - strand at 514.267 kb on - strand at 514.267 kb on - strand at 514.267 kb on - strand at 514.267 kb on - strand at 514.267 kb on - strand at 514.267 kb on - strand at 514.267 kb on - strand at 514.267 kb on - strand at 514.267 kb on - strand at 514.303 kb on + strand at 514.746 kb on + strand, within Psyr_0475 at 514.746 kb on + strand, within Psyr_0475 at 514.746 kb on + strand, within Psyr_0475 at 514.746 kb on + strand, within Psyr_0475 at 514.746 kb on + strand, within Psyr_0475 at 514.746 kb on + strand, within Psyr_0475 at 514.787 kb on + strand, within Psyr_0475 at 514.787 kb on + strand, within Psyr_0475 at 514.787 kb on + strand, within Psyr_0475 at 514.788 kb on - strand, within Psyr_0475 at 514.800 kb on + strand at 514.828 kb on - strand at 514.828 kb on - strand at 514.828 kb on - strand at 514.828 kb on - strand at 514.828 kb on - strand at 515.167 kb on + strand, within Psyr_0476 at 515.496 kb on + strand, within Psyr_0476 at 515.734 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Matrine 10 mM remove 513,298 + Psyr_0474 0.39 -0.0 513,298 + Psyr_0474 0.39 -0.4 513,299 - Psyr_0474 0.39 +0.8 513,299 - Psyr_0474 0.39 +0.2 513,299 - Psyr_0474 0.39 -0.6 513,299 - Psyr_0474 0.39 -0.7 513,723 - Psyr_0474 0.76 +1.1 513,874 + Psyr_0474 0.89 +1.0 513,874 + Psyr_0474 0.89 +0.7 513,875 - Psyr_0474 0.89 +1.0 513,875 - Psyr_0474 0.89 -1.4 513,883 + Psyr_0474 0.90 -0.5 513,883 + Psyr_0474 0.90 -0.5 513,895 + +0.2 513,895 + -0.1 513,896 - -1.0 513,896 - +0.7 513,896 - -0.7 513,988 - -0.2 514,015 + -0.4 514,016 - +1.1 514,026 + +0.2 514,026 + +1.5 514,026 + -0.2 514,026 + +1.4 514,026 + -0.2 514,026 + -0.7 514,026 + +0.3 514,027 - +0.0 514,027 - -1.5 514,027 - -0.7 514,032 + +0.3 514,032 + -0.0 514,058 - -0.3 514,064 + +1.5 514,064 + +0.3 514,092 + -0.8 514,092 + -1.2 514,092 + +0.0 514,092 + -0.7 514,093 - +1.7 514,093 - -2.9 514,096 + +0.1 514,096 + +0.2 514,096 + -0.2 514,097 - -0.7 514,097 - +0.4 514,107 + -0.7 514,107 + -0.2 514,108 - +0.3 514,108 - +0.3 514,108 - +0.0 514,108 - +0.4 514,108 - -2.1 514,146 + -0.0 514,147 - -0.4 514,147 - -0.7 514,148 + -1.4 514,148 + -0.1 514,148 + -0.2 514,148 + +0.2 514,148 + -1.1 514,149 - -0.3 514,149 - -0.7 514,149 - +0.5 514,149 - -0.7 514,149 - -0.4 514,163 + -1.5 514,163 + +1.4 514,163 + +1.0 514,163 + +0.5 514,163 + +0.3 514,163 + -0.7 514,163 + +1.1 514,163 + +1.6 514,164 - +1.3 514,164 - +1.3 514,164 - +1.4 514,164 - -0.0 514,164 - +0.3 514,164 - -0.8 514,164 - -0.0 514,164 - +0.6 514,164 - -0.5 514,164 - +0.1 514,164 - +0.2 514,164 - -0.5 514,164 - -0.4 514,164 - -0.3 514,164 - -0.1 514,167 + +0.4 514,266 + +1.5 514,266 + -0.2 514,266 + +0.0 514,266 + +0.3 514,266 + +0.5 514,266 + +0.5 514,266 + +0.7 514,266 + -0.6 514,266 + -1.1 514,266 + +2.8 514,266 + -0.2 514,266 + +0.3 514,266 + +0.2 514,267 - +0.8 514,267 - -0.4 514,267 - +0.2 514,267 - +0.3 514,267 - -0.6 514,267 - +0.8 514,267 - -0.2 514,267 - +0.2 514,267 - +0.3 514,267 - -1.1 514,267 - -0.1 514,267 - +0.6 514,303 + -0.4 514,746 + Psyr_0475 0.81 +1.3 514,746 + Psyr_0475 0.81 -0.7 514,746 + Psyr_0475 0.81 -0.7 514,746 + Psyr_0475 0.81 +1.3 514,746 + Psyr_0475 0.81 +0.3 514,746 + Psyr_0475 0.81 -0.3 514,787 + Psyr_0475 0.88 -1.3 514,787 + Psyr_0475 0.88 -2.6 514,787 + Psyr_0475 0.88 +0.4 514,788 - Psyr_0475 0.88 +0.3 514,800 + -0.2 514,828 - +0.1 514,828 - +0.8 514,828 - +0.3 514,828 - +0.9 514,828 - -0.3 515,167 + Psyr_0476 0.35 +0.3 515,496 + Psyr_0476 0.75 +0.9 515,734 + -0.3
Or see this region's nucleotide sequence