Experiment: KB with Matrine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0346 and Psyr_0347 are separated by 59 nucleotides Psyr_0347 and Psyr_0348 are separated by 137 nucleotides Psyr_0348 and Psyr_0349 are separated by 56 nucleotides
Psyr_0346: Psyr_0346 - Acriflavin resistance protein, at 368,978 to 372,079
_0346
Psyr_0347: Psyr_0347 - hypothetical protein, at 372,139 to 372,636
_0347
Psyr_0348: Psyr_0348 - conserved hypothetical protein, at 372,774 to 373,400
_0348
Psyr_0349: Psyr_0349 - Binding-protein-dependent transport systems inner membrane component, at 373,457 to 374,101
_0349
Position (kb)
372
373 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 371.213 kb on + strand, within Psyr_0346 at 371.213 kb on + strand, within Psyr_0346 at 371.213 kb on + strand, within Psyr_0346 at 371.214 kb on - strand, within Psyr_0346 at 371.214 kb on - strand, within Psyr_0346 at 371.214 kb on - strand, within Psyr_0346 at 371.214 kb on - strand, within Psyr_0346 at 371.214 kb on - strand, within Psyr_0346 at 371.214 kb on - strand, within Psyr_0346 at 371.214 kb on - strand, within Psyr_0346 at 371.490 kb on - strand, within Psyr_0346 at 371.780 kb on + strand at 371.780 kb on + strand at 371.781 kb on - strand at 371.781 kb on - strand at 371.781 kb on - strand at 371.781 kb on - strand at 371.984 kb on + strand at 371.984 kb on + strand at 371.984 kb on + strand at 371.984 kb on + strand at 371.984 kb on + strand at 371.984 kb on + strand at 371.985 kb on - strand at 371.985 kb on - strand at 371.985 kb on - strand at 372.199 kb on + strand, within Psyr_0347 at 372.247 kb on - strand, within Psyr_0347 at 372.386 kb on + strand, within Psyr_0347 at 372.400 kb on + strand, within Psyr_0347 at 372.400 kb on + strand, within Psyr_0347 at 372.402 kb on + strand, within Psyr_0347 at 372.457 kb on - strand, within Psyr_0347 at 372.519 kb on + strand, within Psyr_0347 at 372.623 kb on + strand at 372.623 kb on + strand at 372.623 kb on + strand at 372.624 kb on - strand at 372.624 kb on - strand at 372.624 kb on - strand at 372.624 kb on - strand at 372.624 kb on - strand at 372.624 kb on - strand at 372.624 kb on - strand at 372.624 kb on - strand at 372.624 kb on - strand at 372.624 kb on - strand at 372.624 kb on - strand at 372.624 kb on - strand at 372.624 kb on - strand at 372.624 kb on - strand at 372.624 kb on - strand at 372.681 kb on + strand at 372.689 kb on + strand at 372.689 kb on + strand at 372.689 kb on + strand at 372.689 kb on + strand at 372.689 kb on + strand at 372.689 kb on + strand at 372.689 kb on + strand at 372.689 kb on + strand at 372.689 kb on + strand at 372.690 kb on - strand at 372.690 kb on - strand at 372.690 kb on - strand at 372.690 kb on - strand at 372.708 kb on + strand at 372.708 kb on + strand at 372.708 kb on + strand at 372.709 kb on - strand at 372.709 kb on - strand at 372.749 kb on + strand at 372.749 kb on + strand at 372.750 kb on - strand at 372.750 kb on - strand at 372.750 kb on - strand at 372.750 kb on - strand at 372.838 kb on + strand, within Psyr_0348 at 372.839 kb on - strand, within Psyr_0348 at 372.841 kb on + strand, within Psyr_0348 at 372.841 kb on + strand, within Psyr_0348 at 372.841 kb on + strand, within Psyr_0348 at 372.841 kb on + strand, within Psyr_0348 at 372.842 kb on - strand, within Psyr_0348 at 372.842 kb on - strand, within Psyr_0348 at 372.842 kb on - strand, within Psyr_0348 at 372.842 kb on - strand, within Psyr_0348 at 372.905 kb on - strand, within Psyr_0348 at 373.287 kb on - strand, within Psyr_0348 at 373.345 kb on + strand at 373.345 kb on + strand at 373.345 kb on + strand at 373.345 kb on + strand at 373.345 kb on + strand at 373.345 kb on + strand at 373.346 kb on - strand at 373.346 kb on - strand at 373.346 kb on - strand at 373.346 kb on - strand at 373.346 kb on - strand at 373.346 kb on - strand at 373.432 kb on + strand at 373.432 kb on + strand at 373.432 kb on + strand at 373.432 kb on + strand at 373.434 kb on + strand at 373.435 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Matrine 10 mM remove 371,213 + Psyr_0346 0.72 +2.7 371,213 + Psyr_0346 0.72 -0.8 371,213 + Psyr_0346 0.72 +0.8 371,214 - Psyr_0346 0.72 +0.5 371,214 - Psyr_0346 0.72 -0.1 371,214 - Psyr_0346 0.72 +0.0 371,214 - Psyr_0346 0.72 +0.6 371,214 - Psyr_0346 0.72 +0.1 371,214 - Psyr_0346 0.72 +0.2 371,214 - Psyr_0346 0.72 -0.3 371,490 - Psyr_0346 0.81 -1.8 371,780 + -0.3 371,780 + +0.4 371,781 - +0.7 371,781 - +1.1 371,781 - +0.9 371,781 - +1.2 371,984 + +0.1 371,984 + +0.2 371,984 + -0.4 371,984 + +0.7 371,984 + +0.1 371,984 + -1.2 371,985 - -1.8 371,985 - -0.2 371,985 - -0.0 372,199 + Psyr_0347 0.12 -1.7 372,247 - Psyr_0347 0.22 -0.0 372,386 + Psyr_0347 0.50 -0.4 372,400 + Psyr_0347 0.52 -1.0 372,400 + Psyr_0347 0.52 -1.8 372,402 + Psyr_0347 0.53 -1.0 372,457 - Psyr_0347 0.64 -0.9 372,519 + Psyr_0347 0.76 -1.3 372,623 + +1.3 372,623 + -0.8 372,623 + -0.8 372,624 - -0.4 372,624 - +0.2 372,624 - +0.9 372,624 - +0.6 372,624 - -1.6 372,624 - +0.2 372,624 - -0.9 372,624 - +0.2 372,624 - +0.1 372,624 - -0.7 372,624 - +0.0 372,624 - -0.7 372,624 - +0.7 372,624 - -0.2 372,624 - +0.9 372,681 + +1.3 372,689 + +1.2 372,689 + -0.4 372,689 + +0.1 372,689 + -1.2 372,689 + +0.7 372,689 + -1.4 372,689 + -0.0 372,689 + +0.1 372,689 + -0.7 372,690 - +0.0 372,690 - -0.1 372,690 - -0.6 372,690 - +0.8 372,708 + -1.2 372,708 + -0.4 372,708 + +0.2 372,709 - +0.3 372,709 - +0.3 372,749 + -0.8 372,749 + +0.4 372,750 - +0.0 372,750 - -0.4 372,750 - +0.7 372,750 - +0.1 372,838 + Psyr_0348 0.10 -0.1 372,839 - Psyr_0348 0.10 +0.3 372,841 + Psyr_0348 0.11 +0.2 372,841 + Psyr_0348 0.11 -1.3 372,841 + Psyr_0348 0.11 -0.1 372,841 + Psyr_0348 0.11 +0.7 372,842 - Psyr_0348 0.11 +0.1 372,842 - Psyr_0348 0.11 -0.0 372,842 - Psyr_0348 0.11 -0.3 372,842 - Psyr_0348 0.11 +0.3 372,905 - Psyr_0348 0.21 -1.2 373,287 - Psyr_0348 0.82 +1.1 373,345 + -0.0 373,345 + +1.3 373,345 + +0.6 373,345 + -0.8 373,345 + +0.0 373,345 + +0.6 373,346 - -1.8 373,346 - -0.0 373,346 - -0.1 373,346 - -0.8 373,346 - -0.1 373,346 - +0.9 373,432 + +0.2 373,432 + +0.2 373,432 + +0.1 373,432 + -0.0 373,434 + -1.8 373,435 - -4.0
Or see this region's nucleotide sequence