Experiment: KB with Matrine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0280 and Psyr_0281 are separated by 307 nucleotides Psyr_0281 and Psyr_0282 overlap by 1 nucleotides Psyr_0282 and Psyr_0283 are separated by 50 nucleotides
Psyr_0280: Psyr_0280 - Catalase, at 297,477 to 299,627
_0280
Psyr_0281: Psyr_0281 - ABC transporter, at 299,935 to 300,942
_0281
Psyr_0282: Psyr_0282 - Binding-protein-dependent transport systems inner membrane component, at 300,942 to 301,616
_0282
Psyr_0283: Psyr_0283 - NLPA lipoprotein, at 301,667 to 302,440
_0283
Position (kb) 299
300
301 Strain fitness (log2 ratio) -2
-1
0
1
2 at 299.158 kb on + strand, within Psyr_0280 at 299.159 kb on - strand, within Psyr_0280 at 299.159 kb on - strand, within Psyr_0280 at 299.283 kb on - strand, within Psyr_0280 at 299.305 kb on + strand, within Psyr_0280 at 299.305 kb on + strand, within Psyr_0280 at 299.305 kb on + strand, within Psyr_0280 at 299.306 kb on - strand, within Psyr_0280 at 299.317 kb on + strand, within Psyr_0280 at 299.317 kb on + strand, within Psyr_0280 at 299.317 kb on + strand, within Psyr_0280 at 299.318 kb on - strand, within Psyr_0280 at 299.318 kb on - strand, within Psyr_0280 at 299.318 kb on - strand, within Psyr_0280 at 299.318 kb on - strand, within Psyr_0280 at 299.363 kb on + strand, within Psyr_0280 at 299.639 kb on + strand at 299.639 kb on + strand at 299.639 kb on + strand at 299.639 kb on + strand at 299.640 kb on - strand at 299.674 kb on + strand at 299.674 kb on + strand at 299.748 kb on - strand at 299.748 kb on - strand at 299.758 kb on + strand at 299.758 kb on + strand at 299.758 kb on + strand at 299.758 kb on + strand at 299.759 kb on - strand at 299.759 kb on - strand at 299.759 kb on - strand at 299.779 kb on + strand at 299.779 kb on + strand at 299.780 kb on - strand at 299.805 kb on - strand at 299.808 kb on + strand at 299.809 kb on - strand at 299.809 kb on - strand at 299.809 kb on - strand at 299.809 kb on - strand at 299.809 kb on - strand at 299.809 kb on - strand at 299.809 kb on - strand at 299.809 kb on - strand at 299.816 kb on - strand at 299.870 kb on + strand at 299.950 kb on - strand at 299.987 kb on + strand at 300.069 kb on + strand, within Psyr_0281 at 300.758 kb on - strand, within Psyr_0281 at 300.758 kb on - strand, within Psyr_0281 at 300.758 kb on - strand, within Psyr_0281 at 300.758 kb on - strand, within Psyr_0281 at 300.822 kb on + strand, within Psyr_0281 at 300.924 kb on + strand at 300.924 kb on + strand at 300.924 kb on + strand at 300.925 kb on - strand at 300.925 kb on - strand at 301.119 kb on + strand, within Psyr_0282 at 301.119 kb on + strand, within Psyr_0282 at 301.119 kb on + strand, within Psyr_0282 at 301.119 kb on + strand, within Psyr_0282 at 301.119 kb on + strand, within Psyr_0282 at 301.120 kb on - strand, within Psyr_0282 at 301.120 kb on - strand, within Psyr_0282 at 301.543 kb on + strand, within Psyr_0282 at 301.544 kb on - strand, within Psyr_0282 at 301.544 kb on - strand, within Psyr_0282 at 301.544 kb on - strand, within Psyr_0282 at 301.544 kb on - strand, within Psyr_0282 at 301.631 kb on - strand at 301.678 kb on - strand at 301.678 kb on - strand at 301.678 kb on - strand at 301.835 kb on + strand, within Psyr_0283 at 301.835 kb on + strand, within Psyr_0283 at 301.835 kb on + strand, within Psyr_0283 at 301.835 kb on + strand, within Psyr_0283 at 301.836 kb on - strand, within Psyr_0283 at 301.836 kb on - strand, within Psyr_0283 at 301.836 kb on - strand, within Psyr_0283 at 301.836 kb on - strand, within Psyr_0283 at 301.836 kb on - strand, within Psyr_0283 at 301.871 kb on - strand, within Psyr_0283
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Matrine 10 mM remove 299,158 + Psyr_0280 0.78 -0.2 299,159 - Psyr_0280 0.78 -0.1 299,159 - Psyr_0280 0.78 -0.2 299,283 - Psyr_0280 0.84 -0.2 299,305 + Psyr_0280 0.85 -0.6 299,305 + Psyr_0280 0.85 -0.5 299,305 + Psyr_0280 0.85 +0.4 299,306 - Psyr_0280 0.85 -0.6 299,317 + Psyr_0280 0.86 +0.4 299,317 + Psyr_0280 0.86 +1.2 299,317 + Psyr_0280 0.86 +0.4 299,318 - Psyr_0280 0.86 +0.3 299,318 - Psyr_0280 0.86 -0.4 299,318 - Psyr_0280 0.86 +1.1 299,318 - Psyr_0280 0.86 -0.2 299,363 + Psyr_0280 0.88 +1.8 299,639 + +0.2 299,639 + +0.0 299,639 + -0.2 299,639 + -1.4 299,640 - -1.0 299,674 + +0.5 299,674 + +0.5 299,748 - +0.2 299,748 - -0.1 299,758 + -0.1 299,758 + -0.5 299,758 + -0.2 299,758 + -0.9 299,759 - -0.1 299,759 - +0.5 299,759 - -0.3 299,779 + +0.6 299,779 + +0.4 299,780 - +0.1 299,805 - +0.8 299,808 + -0.1 299,809 - +0.3 299,809 - -2.1 299,809 - -0.7 299,809 - -0.7 299,809 - -1.1 299,809 - -1.0 299,809 - -0.2 299,809 - -0.3 299,816 - -0.0 299,870 + -0.6 299,950 - -0.9 299,987 + -0.4 300,069 + Psyr_0281 0.13 -0.2 300,758 - Psyr_0281 0.82 -2.6 300,758 - Psyr_0281 0.82 +0.1 300,758 - Psyr_0281 0.82 -1.2 300,758 - Psyr_0281 0.82 -0.2 300,822 + Psyr_0281 0.88 -0.4 300,924 + -0.4 300,924 + -0.3 300,924 + -0.5 300,925 - -2.5 300,925 - -2.7 301,119 + Psyr_0282 0.26 -0.6 301,119 + Psyr_0282 0.26 -2.4 301,119 + Psyr_0282 0.26 -1.3 301,119 + Psyr_0282 0.26 +0.0 301,119 + Psyr_0282 0.26 -1.1 301,120 - Psyr_0282 0.26 -1.0 301,120 - Psyr_0282 0.26 -0.4 301,543 + Psyr_0282 0.89 -0.3 301,544 - Psyr_0282 0.89 -1.8 301,544 - Psyr_0282 0.89 -0.1 301,544 - Psyr_0282 0.89 -1.2 301,544 - Psyr_0282 0.89 +0.2 301,631 - +0.3 301,678 - +0.5 301,678 - +0.1 301,678 - +0.3 301,835 + Psyr_0283 0.22 -1.5 301,835 + Psyr_0283 0.22 -0.4 301,835 + Psyr_0283 0.22 +1.7 301,835 + Psyr_0283 0.22 +0.5 301,836 - Psyr_0283 0.22 +0.4 301,836 - Psyr_0283 0.22 +1.3 301,836 - Psyr_0283 0.22 -0.2 301,836 - Psyr_0283 0.22 +0.4 301,836 - Psyr_0283 0.22 +0.2 301,871 - Psyr_0283 0.26 +0.7
Or see this region's nucleotide sequence