Experiment: KB with Matrine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0269 and Psyr_0270 are separated by 241 nucleotides Psyr_0270 and Psyr_0271 are separated by 74 nucleotides Psyr_0271 and Psyr_0272 are separated by 36 nucleotides
Psyr_0269: Psyr_0269 - cell division checkpoint GTPase YihA, at 286,741 to 287,448
_0269
Psyr_0270: Psyr_0270 - DNA polymerase I, at 287,690 to 290,455
_0270
Psyr_0271: Psyr_0271 - conserved hypothetical protein, at 290,530 to 290,817
_0271
Psyr_0272: Psyr_0272 - homoserine kinase, at 290,854 to 291,804
_0272
Position (kb) 287
288
289
290
291 Strain fitness (log2 ratio) -2
-1
0
1
2 at 286.701 kb on + strand at 286.701 kb on + strand at 286.738 kb on + strand at 286.738 kb on + strand at 286.738 kb on + strand at 286.738 kb on + strand at 286.738 kb on + strand at 286.740 kb on + strand at 286.740 kb on + strand at 286.740 kb on + strand at 286.740 kb on + strand at 286.740 kb on + strand at 286.740 kb on + strand at 286.740 kb on + strand at 286.740 kb on + strand at 286.741 kb on + strand at 287.446 kb on + strand at 287.446 kb on + strand at 287.504 kb on + strand at 287.504 kb on + strand at 287.505 kb on - strand at 287.548 kb on + strand at 287.548 kb on + strand at 287.548 kb on + strand at 287.548 kb on + strand at 287.549 kb on - strand at 287.549 kb on - strand at 287.549 kb on - strand at 287.549 kb on - strand at 287.549 kb on - strand at 287.549 kb on - strand at 287.557 kb on + strand at 287.557 kb on + strand at 287.557 kb on + strand at 287.557 kb on + strand at 287.557 kb on + strand at 287.557 kb on + strand at 287.557 kb on + strand at 287.558 kb on - strand at 287.558 kb on - strand at 287.558 kb on - strand at 287.558 kb on - strand at 287.558 kb on - strand at 287.614 kb on - strand at 287.647 kb on + strand at 287.652 kb on + strand at 287.653 kb on - strand at 287.653 kb on - strand at 287.653 kb on - strand at 287.653 kb on - strand at 287.730 kb on - strand at 287.730 kb on - strand at 287.838 kb on - strand at 288.990 kb on + strand, within Psyr_0270 at 289.086 kb on + strand, within Psyr_0270 at 289.087 kb on - strand, within Psyr_0270 at 289.137 kb on - strand, within Psyr_0270 at 289.140 kb on + strand, within Psyr_0270 at 289.140 kb on + strand, within Psyr_0270 at 289.140 kb on + strand, within Psyr_0270 at 289.141 kb on - strand, within Psyr_0270 at 289.141 kb on - strand, within Psyr_0270 at 289.141 kb on - strand, within Psyr_0270 at 289.141 kb on - strand, within Psyr_0270 at 290.540 kb on + strand at 290.540 kb on + strand at 290.541 kb on - strand at 290.541 kb on - strand at 290.628 kb on + strand, within Psyr_0271 at 290.628 kb on + strand, within Psyr_0271 at 290.628 kb on + strand, within Psyr_0271 at 290.628 kb on + strand, within Psyr_0271 at 290.631 kb on + strand, within Psyr_0271 at 290.631 kb on + strand, within Psyr_0271 at 290.631 kb on + strand, within Psyr_0271 at 290.631 kb on + strand, within Psyr_0271 at 290.687 kb on - strand, within Psyr_0271 at 290.687 kb on - strand, within Psyr_0271 at 290.687 kb on - strand, within Psyr_0271 at 290.745 kb on + strand, within Psyr_0271 at 290.745 kb on + strand, within Psyr_0271 at 290.804 kb on + strand at 290.823 kb on + strand at 290.823 kb on + strand at 290.823 kb on + strand at 290.823 kb on + strand at 290.823 kb on + strand at 290.823 kb on + strand at 290.823 kb on + strand at 290.823 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Matrine 10 mM remove 286,701 + +0.6 286,701 + +0.3 286,738 + -0.6 286,738 + +0.4 286,738 + -0.1 286,738 + +0.5 286,738 + +0.4 286,740 + -1.0 286,740 + -0.6 286,740 + -0.8 286,740 + -0.1 286,740 + +0.1 286,740 + -0.9 286,740 + -0.5 286,740 + -0.0 286,741 + -0.0 287,446 + +1.0 287,446 + -0.1 287,504 + -1.1 287,504 + -0.0 287,505 - -1.9 287,548 + -0.9 287,548 + -0.6 287,548 + -0.1 287,548 + +1.8 287,549 - +0.0 287,549 - +0.0 287,549 - +1.1 287,549 - +0.1 287,549 - -0.0 287,549 - -0.4 287,557 + +0.6 287,557 + -1.0 287,557 + +2.1 287,557 + +0.4 287,557 + -1.1 287,557 + +0.9 287,557 + -0.8 287,558 - +0.4 287,558 - +0.6 287,558 - -1.2 287,558 - -1.0 287,558 - -0.5 287,614 - +0.1 287,647 + -0.1 287,652 + +0.9 287,653 - -1.1 287,653 - +0.9 287,653 - -1.6 287,653 - +0.0 287,730 - -0.0 287,730 - -1.1 287,838 - -0.3 288,990 + Psyr_0270 0.47 -1.6 289,086 + Psyr_0270 0.50 +0.0 289,087 - Psyr_0270 0.51 -1.5 289,137 - Psyr_0270 0.52 -0.9 289,140 + Psyr_0270 0.52 -1.1 289,140 + Psyr_0270 0.52 +0.1 289,140 + Psyr_0270 0.52 -0.6 289,141 - Psyr_0270 0.52 -1.2 289,141 - Psyr_0270 0.52 -2.2 289,141 - Psyr_0270 0.52 -0.0 289,141 - Psyr_0270 0.52 -0.3 290,540 + -0.1 290,540 + -0.6 290,541 - +0.7 290,541 - -0.7 290,628 + Psyr_0271 0.34 +0.5 290,628 + Psyr_0271 0.34 +0.3 290,628 + Psyr_0271 0.34 +0.4 290,628 + Psyr_0271 0.34 +1.1 290,631 + Psyr_0271 0.35 +0.5 290,631 + Psyr_0271 0.35 +0.1 290,631 + Psyr_0271 0.35 +0.9 290,631 + Psyr_0271 0.35 -0.3 290,687 - Psyr_0271 0.55 -0.7 290,687 - Psyr_0271 0.55 +0.2 290,687 - Psyr_0271 0.55 -0.6 290,745 + Psyr_0271 0.75 +1.6 290,745 + Psyr_0271 0.75 -1.1 290,804 + -0.5 290,823 + +1.4 290,823 + -0.2 290,823 + +0.3 290,823 + -0.9 290,823 + -0.1 290,823 + +0.2 290,823 + +0.1 290,823 + -0.3
Or see this region's nucleotide sequence