Experiment: KB with Matrine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0173 and Psyr_0174 are separated by 456 nucleotides Psyr_0174 and Psyr_0175 are separated by 90 nucleotides Psyr_0175 and Psyr_0176 are separated by 216 nucleotides
Psyr_0173: Psyr_0173 - SSU ribosomal protein S6P modification protein, at 186,867 to 187,772
_0173
Psyr_0174: Psyr_0174 - Sodium:dicarboxylate symporter, at 188,229 to 189,560
_0174
Psyr_0175: Psyr_0175 - conserved hypothetical protein, at 189,651 to 189,926
_0175
Psyr_0176: Psyr_0176 - conserved hypothetical protein, at 190,143 to 190,391
_0176
Position (kb) 188
189
190 Strain fitness (log2 ratio) -2
-1
0
1
2 at 187.401 kb on + strand, within Psyr_0173 at 187.401 kb on + strand, within Psyr_0173 at 187.401 kb on + strand, within Psyr_0173 at 187.401 kb on + strand, within Psyr_0173 at 187.401 kb on + strand, within Psyr_0173 at 187.401 kb on + strand, within Psyr_0173 at 187.401 kb on + strand, within Psyr_0173 at 187.401 kb on + strand, within Psyr_0173 at 187.401 kb on + strand, within Psyr_0173 at 187.401 kb on + strand, within Psyr_0173 at 187.402 kb on - strand, within Psyr_0173 at 187.402 kb on - strand, within Psyr_0173 at 187.402 kb on - strand, within Psyr_0173 at 187.402 kb on - strand, within Psyr_0173 at 187.402 kb on - strand, within Psyr_0173 at 187.402 kb on - strand, within Psyr_0173 at 187.402 kb on - strand, within Psyr_0173 at 187.402 kb on - strand, within Psyr_0173 at 187.843 kb on - strand at 187.847 kb on + strand at 187.847 kb on + strand at 187.847 kb on + strand at 187.847 kb on + strand at 187.847 kb on + strand at 187.847 kb on + strand at 187.847 kb on + strand at 187.847 kb on + strand at 187.847 kb on + strand at 187.847 kb on + strand at 187.847 kb on + strand at 187.848 kb on - strand at 187.848 kb on - strand at 187.848 kb on - strand at 187.848 kb on - strand at 187.848 kb on - strand at 187.848 kb on - strand at 187.848 kb on - strand at 187.848 kb on - strand at 187.848 kb on - strand at 187.848 kb on - strand at 187.856 kb on + strand at 187.935 kb on + strand at 187.935 kb on + strand at 187.935 kb on + strand at 187.935 kb on + strand at 187.936 kb on - strand at 187.936 kb on - strand at 187.936 kb on - strand at 187.978 kb on + strand at 187.979 kb on - strand at 188.047 kb on + strand at 188.047 kb on + strand at 188.047 kb on + strand at 188.047 kb on + strand at 188.047 kb on + strand at 188.047 kb on + strand at 188.048 kb on - strand at 188.048 kb on - strand at 188.048 kb on - strand at 188.048 kb on - strand at 188.048 kb on - strand at 188.050 kb on - strand at 188.066 kb on + strand at 188.066 kb on + strand at 188.067 kb on - strand at 188.067 kb on - strand at 188.089 kb on + strand at 188.222 kb on + strand at 188.223 kb on - strand at 188.223 kb on - strand at 188.225 kb on + strand at 188.225 kb on + strand at 188.225 kb on + strand at 188.226 kb on - strand at 188.226 kb on - strand at 188.226 kb on - strand at 188.226 kb on - strand at 188.226 kb on - strand at 188.226 kb on - strand at 188.226 kb on - strand at 188.228 kb on + strand at 188.228 kb on + strand at 188.228 kb on + strand at 188.229 kb on - strand at 188.229 kb on - strand at 188.229 kb on - strand at 188.367 kb on - strand, within Psyr_0174 at 188.396 kb on + strand, within Psyr_0174 at 188.465 kb on + strand, within Psyr_0174 at 188.465 kb on + strand, within Psyr_0174 at 188.488 kb on - strand, within Psyr_0174 at 188.488 kb on - strand, within Psyr_0174 at 188.488 kb on - strand, within Psyr_0174 at 188.601 kb on + strand, within Psyr_0174 at 188.602 kb on - strand, within Psyr_0174 at 188.841 kb on + strand, within Psyr_0174 at 188.842 kb on - strand, within Psyr_0174 at 188.938 kb on - strand, within Psyr_0174 at 189.020 kb on + strand, within Psyr_0174 at 189.020 kb on + strand, within Psyr_0174 at 189.021 kb on - strand, within Psyr_0174 at 189.021 kb on - strand, within Psyr_0174 at 189.021 kb on - strand, within Psyr_0174 at 189.048 kb on + strand, within Psyr_0174 at 189.105 kb on + strand, within Psyr_0174 at 189.106 kb on - strand, within Psyr_0174 at 189.106 kb on - strand, within Psyr_0174 at 189.106 kb on - strand, within Psyr_0174 at 189.180 kb on + strand, within Psyr_0174 at 189.180 kb on + strand, within Psyr_0174 at 189.180 kb on + strand, within Psyr_0174 at 189.447 kb on - strand at 189.447 kb on - strand at 189.468 kb on + strand at 189.468 kb on + strand at 189.468 kb on + strand at 189.468 kb on + strand at 189.468 kb on + strand at 189.468 kb on + strand at 189.468 kb on + strand at 189.468 kb on + strand at 189.468 kb on + strand at 189.468 kb on + strand at 189.468 kb on + strand at 189.468 kb on + strand at 189.469 kb on - strand at 189.469 kb on - strand at 189.469 kb on - strand at 189.469 kb on - strand at 189.469 kb on - strand at 189.469 kb on - strand at 189.469 kb on - strand at 189.469 kb on - strand at 189.469 kb on - strand at 189.469 kb on - strand at 189.469 kb on - strand at 189.469 kb on - strand at 189.469 kb on - strand at 189.469 kb on - strand at 189.469 kb on - strand at 189.469 kb on - strand at 189.469 kb on - strand at 189.568 kb on + strand at 189.568 kb on + strand at 189.568 kb on + strand at 189.568 kb on + strand at 189.569 kb on - strand at 189.569 kb on - strand at 189.635 kb on + strand at 189.783 kb on + strand, within Psyr_0175 at 189.783 kb on + strand, within Psyr_0175 at 189.783 kb on + strand, within Psyr_0175 at 189.783 kb on + strand, within Psyr_0175 at 189.783 kb on + strand, within Psyr_0175 at 189.783 kb on + strand, within Psyr_0175 at 189.783 kb on + strand, within Psyr_0175 at 189.783 kb on + strand, within Psyr_0175 at 189.783 kb on + strand, within Psyr_0175 at 189.783 kb on + strand, within Psyr_0175 at 189.784 kb on - strand, within Psyr_0175 at 189.784 kb on - strand, within Psyr_0175 at 189.784 kb on - strand, within Psyr_0175 at 189.784 kb on - strand, within Psyr_0175 at 189.784 kb on - strand, within Psyr_0175 at 189.798 kb on - strand, within Psyr_0175 at 189.798 kb on - strand, within Psyr_0175 at 189.880 kb on - strand, within Psyr_0175 at 189.880 kb on - strand, within Psyr_0175 at 189.880 kb on - strand, within Psyr_0175 at 189.954 kb on + strand at 189.954 kb on + strand at 189.954 kb on + strand at 189.954 kb on + strand at 189.954 kb on + strand at 189.954 kb on + strand at 189.954 kb on + strand at 189.954 kb on + strand at 189.954 kb on + strand at 189.954 kb on + strand at 189.954 kb on + strand at 189.954 kb on + strand at 189.954 kb on + strand at 189.954 kb on + strand at 189.954 kb on + strand at 189.954 kb on + strand at 189.955 kb on - strand at 189.955 kb on - strand at 189.955 kb on - strand at 189.955 kb on - strand at 189.955 kb on - strand at 189.955 kb on - strand at 189.955 kb on - strand at 189.955 kb on - strand at 189.955 kb on - strand at 189.955 kb on - strand at 189.955 kb on - strand at 189.955 kb on - strand at 189.955 kb on - strand at 189.955 kb on - strand at 189.955 kb on - strand at 189.956 kb on + strand at 189.956 kb on + strand at 189.956 kb on + strand at 189.956 kb on + strand at 189.956 kb on + strand at 189.956 kb on + strand at 189.956 kb on + strand at 189.956 kb on + strand at 189.956 kb on + strand at 189.956 kb on + strand at 189.956 kb on + strand at 189.956 kb on + strand at 189.956 kb on + strand at 189.956 kb on + strand at 189.956 kb on + strand at 189.956 kb on + strand at 189.956 kb on + strand at 189.956 kb on + strand at 189.957 kb on - strand at 189.957 kb on - strand at 189.957 kb on - strand at 189.957 kb on - strand at 189.957 kb on - strand at 189.957 kb on - strand at 189.957 kb on - strand at 189.957 kb on - strand at 189.957 kb on - strand at 189.957 kb on - strand at 189.957 kb on - strand at 189.957 kb on - strand at 189.957 kb on - strand at 189.957 kb on - strand at 189.957 kb on - strand at 189.957 kb on - strand at 189.957 kb on - strand at 189.957 kb on - strand at 189.957 kb on - strand at 189.957 kb on - strand at 189.957 kb on - strand at 189.957 kb on - strand at 189.957 kb on - strand at 189.957 kb on - strand at 189.957 kb on - strand at 189.983 kb on + strand at 189.983 kb on + strand at 189.983 kb on + strand at 189.984 kb on - strand at 189.984 kb on - strand at 190.116 kb on + strand at 190.116 kb on + strand at 190.116 kb on + strand at 190.116 kb on + strand at 190.116 kb on + strand at 190.117 kb on - strand at 190.117 kb on - strand at 190.117 kb on - strand at 190.117 kb on - strand at 190.117 kb on - strand at 190.119 kb on + strand at 190.119 kb on + strand at 190.119 kb on + strand at 190.120 kb on - strand at 190.120 kb on - strand at 190.120 kb on - strand at 190.120 kb on - strand at 190.123 kb on - strand at 190.138 kb on + strand at 190.138 kb on + strand at 190.139 kb on - strand at 190.139 kb on - strand at 190.142 kb on + strand at 190.142 kb on + strand at 190.142 kb on + strand at 190.142 kb on + strand at 190.142 kb on + strand at 190.142 kb on + strand at 190.142 kb on + strand at 190.142 kb on + strand at 190.142 kb on + strand at 190.142 kb on + strand at 190.142 kb on + strand at 190.142 kb on + strand at 190.143 kb on - strand at 190.143 kb on - strand at 190.143 kb on - strand at 190.143 kb on - strand at 190.143 kb on - strand at 190.143 kb on - strand at 190.143 kb on - strand at 190.143 kb on - strand at 190.143 kb on - strand at 190.143 kb on - strand at 190.143 kb on - strand at 190.149 kb on + strand at 190.149 kb on + strand at 190.149 kb on + strand at 190.149 kb on + strand at 190.150 kb on - strand at 190.152 kb on - strand at 190.235 kb on - strand, within Psyr_0176 at 190.235 kb on - strand, within Psyr_0176 at 190.235 kb on - strand, within Psyr_0176 at 190.437 kb on + strand at 190.477 kb on - strand at 190.477 kb on - strand at 190.536 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Matrine 10 mM remove 187,401 + Psyr_0173 0.59 +0.9 187,401 + Psyr_0173 0.59 +2.4 187,401 + Psyr_0173 0.59 +0.3 187,401 + Psyr_0173 0.59 +0.9 187,401 + Psyr_0173 0.59 -0.8 187,401 + Psyr_0173 0.59 +1.4 187,401 + Psyr_0173 0.59 -0.5 187,401 + Psyr_0173 0.59 +0.2 187,401 + Psyr_0173 0.59 +0.3 187,401 + Psyr_0173 0.59 -1.7 187,402 - Psyr_0173 0.59 +0.4 187,402 - Psyr_0173 0.59 -0.3 187,402 - Psyr_0173 0.59 -1.2 187,402 - Psyr_0173 0.59 +0.4 187,402 - Psyr_0173 0.59 +1.5 187,402 - Psyr_0173 0.59 -0.6 187,402 - Psyr_0173 0.59 -0.5 187,402 - Psyr_0173 0.59 +0.0 187,843 - -0.7 187,847 + +1.4 187,847 + -1.0 187,847 + -0.5 187,847 + +0.5 187,847 + -0.2 187,847 + -1.3 187,847 + -0.9 187,847 + -0.8 187,847 + +0.7 187,847 + +0.2 187,847 + -0.6 187,848 - -0.8 187,848 - -0.2 187,848 - +0.4 187,848 - +1.1 187,848 - +0.1 187,848 - +0.2 187,848 - -1.6 187,848 - +0.0 187,848 - +0.3 187,848 - +2.0 187,856 + -0.5 187,935 + -0.7 187,935 + -0.6 187,935 + +0.9 187,935 + +0.8 187,936 - +0.4 187,936 - -0.0 187,936 - -1.3 187,978 + +0.2 187,979 - -0.3 188,047 + -0.1 188,047 + -0.3 188,047 + -1.0 188,047 + -0.1 188,047 + +0.2 188,047 + -0.2 188,048 - -0.1 188,048 - -0.0 188,048 - -0.2 188,048 - +0.5 188,048 - -0.0 188,050 - +0.1 188,066 + -0.4 188,066 + +0.6 188,067 - +0.4 188,067 - +0.6 188,089 + +0.5 188,222 + -0.5 188,223 - +1.1 188,223 - +0.2 188,225 + -0.4 188,225 + -0.8 188,225 + +0.1 188,226 - -0.2 188,226 - -1.3 188,226 - +0.9 188,226 - -0.4 188,226 - -0.1 188,226 - -0.6 188,226 - -1.1 188,228 + +0.7 188,228 + +1.4 188,228 + -0.5 188,229 - -0.2 188,229 - +1.1 188,229 - -2.0 188,367 - Psyr_0174 0.10 -0.3 188,396 + Psyr_0174 0.13 -0.7 188,465 + Psyr_0174 0.18 -1.3 188,465 + Psyr_0174 0.18 -1.4 188,488 - Psyr_0174 0.19 -0.0 188,488 - Psyr_0174 0.19 -1.9 188,488 - Psyr_0174 0.19 -1.2 188,601 + Psyr_0174 0.28 -0.2 188,602 - Psyr_0174 0.28 -0.0 188,841 + Psyr_0174 0.46 -1.4 188,842 - Psyr_0174 0.46 -0.1 188,938 - Psyr_0174 0.53 -0.4 189,020 + Psyr_0174 0.59 -0.3 189,020 + Psyr_0174 0.59 +0.1 189,021 - Psyr_0174 0.59 +0.9 189,021 - Psyr_0174 0.59 -2.5 189,021 - Psyr_0174 0.59 -1.1 189,048 + Psyr_0174 0.61 -0.4 189,105 + Psyr_0174 0.66 -0.7 189,106 - Psyr_0174 0.66 -0.3 189,106 - Psyr_0174 0.66 -1.4 189,106 - Psyr_0174 0.66 +0.9 189,180 + Psyr_0174 0.71 -2.1 189,180 + Psyr_0174 0.71 -1.2 189,180 + Psyr_0174 0.71 -1.7 189,447 - -0.6 189,447 - -0.9 189,468 + -0.5 189,468 + -2.4 189,468 + -0.6 189,468 + -0.0 189,468 + +0.4 189,468 + -0.7 189,468 + +0.2 189,468 + -0.1 189,468 + +0.9 189,468 + +0.4 189,468 + +0.7 189,468 + -1.1 189,469 - -0.3 189,469 - +0.1 189,469 - +0.6 189,469 - +0.4 189,469 - +0.7 189,469 - -0.4 189,469 - -2.4 189,469 - +0.1 189,469 - +1.1 189,469 - -1.9 189,469 - +2.4 189,469 - -0.3 189,469 - +0.2 189,469 - -1.0 189,469 - -0.1 189,469 - -1.9 189,469 - -0.5 189,568 + -0.6 189,568 + +0.2 189,568 + -0.0 189,568 + +1.2 189,569 - +0.8 189,569 - +1.1 189,635 + -0.3 189,783 + Psyr_0175 0.48 +0.5 189,783 + Psyr_0175 0.48 -0.2 189,783 + Psyr_0175 0.48 -0.0 189,783 + Psyr_0175 0.48 +0.4 189,783 + Psyr_0175 0.48 -2.1 189,783 + Psyr_0175 0.48 -0.5 189,783 + Psyr_0175 0.48 -1.1 189,783 + Psyr_0175 0.48 +0.2 189,783 + Psyr_0175 0.48 +1.2 189,783 + Psyr_0175 0.48 +1.0 189,784 - Psyr_0175 0.48 -0.4 189,784 - Psyr_0175 0.48 -0.9 189,784 - Psyr_0175 0.48 -0.2 189,784 - Psyr_0175 0.48 -1.2 189,784 - Psyr_0175 0.48 +1.1 189,798 - Psyr_0175 0.53 -0.9 189,798 - Psyr_0175 0.53 -0.1 189,880 - Psyr_0175 0.83 -0.2 189,880 - Psyr_0175 0.83 -0.0 189,880 - Psyr_0175 0.83 -1.7 189,954 + +0.4 189,954 + +0.4 189,954 + -1.3 189,954 + -1.6 189,954 + +2.7 189,954 + -0.6 189,954 + -0.4 189,954 + -0.9 189,954 + +0.3 189,954 + +0.2 189,954 + +0.3 189,954 + -0.8 189,954 + -0.6 189,954 + +1.9 189,954 + +0.3 189,954 + +0.5 189,955 - +1.2 189,955 - -2.1 189,955 - -0.4 189,955 - +0.5 189,955 - +0.2 189,955 - +2.1 189,955 - +0.6 189,955 - +0.5 189,955 - -0.3 189,955 - -0.3 189,955 - -0.0 189,955 - +1.1 189,955 - +0.4 189,955 - +0.2 189,955 - -0.6 189,956 + -0.7 189,956 + -2.2 189,956 + -0.8 189,956 + -0.4 189,956 + -0.4 189,956 + -0.6 189,956 + +0.1 189,956 + +0.6 189,956 + -0.3 189,956 + +0.6 189,956 + +0.4 189,956 + -0.0 189,956 + +0.1 189,956 + +0.1 189,956 + +0.8 189,956 + -0.6 189,956 + +0.4 189,956 + +0.8 189,957 - -0.2 189,957 - -0.2 189,957 - -0.7 189,957 - -0.2 189,957 - +1.7 189,957 - +0.1 189,957 - +0.7 189,957 - +0.5 189,957 - -0.5 189,957 - +0.2 189,957 - +0.2 189,957 - +0.9 189,957 - -0.7 189,957 - -0.7 189,957 - +1.1 189,957 - -0.6 189,957 - +0.2 189,957 - +0.2 189,957 - +0.3 189,957 - -1.1 189,957 - +1.1 189,957 - +0.0 189,957 - -1.0 189,957 - +1.0 189,957 - -0.0 189,983 + +0.6 189,983 + -0.4 189,983 + +0.4 189,984 - +0.4 189,984 - -0.4 190,116 + -0.3 190,116 + +0.7 190,116 + +1.0 190,116 + -0.9 190,116 + -2.6 190,117 - +0.0 190,117 - +0.5 190,117 - -0.0 190,117 - +0.3 190,117 - +0.5 190,119 + -0.6 190,119 + -0.3 190,119 + +0.4 190,120 - +0.7 190,120 - -0.4 190,120 - -0.9 190,120 - +0.3 190,123 - -0.5 190,138 + +1.6 190,138 + +0.6 190,139 - -0.9 190,139 - -0.3 190,142 + +0.0 190,142 + +1.0 190,142 + +0.9 190,142 + -1.1 190,142 + -1.9 190,142 + -0.5 190,142 + -2.6 190,142 + -0.9 190,142 + +0.0 190,142 + +0.4 190,142 + -0.1 190,142 + -0.4 190,143 - -0.3 190,143 - +0.0 190,143 - -0.1 190,143 - -0.4 190,143 - -0.3 190,143 - -0.5 190,143 - -0.9 190,143 - -0.2 190,143 - -1.0 190,143 - -0.2 190,143 - +0.4 190,149 + +0.3 190,149 + -0.7 190,149 + -0.2 190,149 + -1.6 190,150 - -0.9 190,152 - +0.3 190,235 - Psyr_0176 0.37 +1.2 190,235 - Psyr_0176 0.37 -0.2 190,235 - Psyr_0176 0.37 +0.3 190,437 + +0.5 190,477 - -0.3 190,477 - -0.4 190,536 - -0.3
Or see this region's nucleotide sequence