Strain Fitness in Pseudomonas syringae pv. syringae B728a around Psyr_0153

Experiment: KB with Matrine 10 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsyr_0152 and Psyr_0153 overlap by 22 nucleotidesPsyr_0153 and Psyr_0154 overlap by 4 nucleotides Psyr_0152: Psyr_0152 - FAD-dependent pyridine nucleotide-disulfide oxidoreductase:BFD-like [2Fe-2S]-binding region, at 165,081 to 166,490 _0152 Psyr_0153: Psyr_0153 - conserved hypothetical protein, at 166,469 to 166,780 _0153 Psyr_0154: Psyr_0154 - Oligopeptide/dipeptide ABC transporter, ATP-binding protein, C-terminal, at 166,777 to 168,618 _0154 Position (kb) 166 167Strain fitness (log2 ratio) -2 -1 0 1at 165.575 kb on + strand, within Psyr_0152at 165.575 kb on + strand, within Psyr_0152at 165.575 kb on + strand, within Psyr_0152at 165.576 kb on - strand, within Psyr_0152at 165.576 kb on - strand, within Psyr_0152at 165.576 kb on - strand, within Psyr_0152at 165.630 kb on + strand, within Psyr_0152at 165.631 kb on - strand, within Psyr_0152at 165.820 kb on + strand, within Psyr_0152at 165.820 kb on + strand, within Psyr_0152at 165.821 kb on - strand, within Psyr_0152at 165.821 kb on - strand, within Psyr_0152at 165.821 kb on - strand, within Psyr_0152at 165.821 kb on - strand, within Psyr_0152at 165.821 kb on - strand, within Psyr_0152at 165.821 kb on - strand, within Psyr_0152at 165.952 kb on + strand, within Psyr_0152at 165.953 kb on - strand, within Psyr_0152at 165.970 kb on + strand, within Psyr_0152at 165.970 kb on + strand, within Psyr_0152at 165.970 kb on + strand, within Psyr_0152at 165.970 kb on + strand, within Psyr_0152at 165.971 kb on - strand, within Psyr_0152at 166.060 kb on + strand, within Psyr_0152at 166.060 kb on + strand, within Psyr_0152at 166.061 kb on - strand, within Psyr_0152at 166.061 kb on - strand, within Psyr_0152at 166.061 kb on - strand, within Psyr_0152at 166.061 kb on - strand, within Psyr_0152at 166.216 kb on + strand, within Psyr_0152at 166.285 kb on + strand, within Psyr_0152at 166.285 kb on + strand, within Psyr_0152at 166.285 kb on + strand, within Psyr_0152at 166.286 kb on - strand, within Psyr_0152at 166.330 kb on + strand, within Psyr_0152at 166.331 kb on - strand, within Psyr_0152at 166.331 kb on - strand, within Psyr_0152at 166.649 kb on + strand, within Psyr_0153at 166.649 kb on + strand, within Psyr_0153at 166.650 kb on - strand, within Psyr_0153at 166.650 kb on - strand, within Psyr_0153at 166.734 kb on - strand, within Psyr_0153at 166.734 kb on - strand, within Psyr_0153at 166.734 kb on - strand, within Psyr_0153at 166.784 kb on + strandat 166.784 kb on + strandat 167.063 kb on + strand, within Psyr_0154at 167.064 kb on - strand, within Psyr_0154at 167.064 kb on - strand, within Psyr_0154at 167.126 kb on + strand, within Psyr_0154at 167.126 kb on + strand, within Psyr_0154at 167.126 kb on + strand, within Psyr_0154at 167.127 kb on - strand, within Psyr_0154at 167.127 kb on - strand, within Psyr_0154at 167.127 kb on - strand, within Psyr_0154at 167.127 kb on - strand, within Psyr_0154at 167.127 kb on - strand, within Psyr_0154at 167.127 kb on - strand, within Psyr_0154at 167.127 kb on - strand, within Psyr_0154at 167.127 kb on - strand, within Psyr_0154at 167.127 kb on - strand, within Psyr_0154at 167.190 kb on - strand, within Psyr_0154at 167.254 kb on - strand, within Psyr_0154at 167.447 kb on + strand, within Psyr_0154at 167.447 kb on + strand, within Psyr_0154at 167.447 kb on + strand, within Psyr_0154at 167.447 kb on + strand, within Psyr_0154at 167.447 kb on + strand, within Psyr_0154at 167.447 kb on + strand, within Psyr_0154at 167.447 kb on + strand, within Psyr_0154at 167.448 kb on - strand, within Psyr_0154at 167.448 kb on - strand, within Psyr_0154at 167.448 kb on - strand, within Psyr_0154at 167.448 kb on - strand, within Psyr_0154at 167.448 kb on - strand, within Psyr_0154

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Per-strain Table

Position Strand Gene LocusTag Fraction KB with Matrine 10 mM
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165,575 + Psyr_0152 0.35 +1.4
165,575 + Psyr_0152 0.35 -1.1
165,575 + Psyr_0152 0.35 +1.1
165,576 - Psyr_0152 0.35 -0.4
165,576 - Psyr_0152 0.35 -0.3
165,576 - Psyr_0152 0.35 +0.5
165,630 + Psyr_0152 0.39 +0.8
165,631 - Psyr_0152 0.39 -0.9
165,820 + Psyr_0152 0.52 +0.4
165,820 + Psyr_0152 0.52 -0.2
165,821 - Psyr_0152 0.52 +0.3
165,821 - Psyr_0152 0.52 -0.4
165,821 - Psyr_0152 0.52 -0.2
165,821 - Psyr_0152 0.52 -0.1
165,821 - Psyr_0152 0.52 +0.4
165,821 - Psyr_0152 0.52 -0.3
165,952 + Psyr_0152 0.62 -0.6
165,953 - Psyr_0152 0.62 -0.8
165,970 + Psyr_0152 0.63 +0.1
165,970 + Psyr_0152 0.63 +0.8
165,970 + Psyr_0152 0.63 -1.3
165,970 + Psyr_0152 0.63 +1.4
165,971 - Psyr_0152 0.63 -0.9
166,060 + Psyr_0152 0.69 +0.2
166,060 + Psyr_0152 0.69 +1.1
166,061 - Psyr_0152 0.70 -0.9
166,061 - Psyr_0152 0.70 -0.7
166,061 - Psyr_0152 0.70 -0.6
166,061 - Psyr_0152 0.70 +0.3
166,216 + Psyr_0152 0.80 +0.3
166,285 + Psyr_0152 0.85 -0.5
166,285 + Psyr_0152 0.85 +0.5
166,285 + Psyr_0152 0.85 +0.7
166,286 - Psyr_0152 0.85 +0.4
166,330 + Psyr_0152 0.89 -0.9
166,331 - Psyr_0152 0.89 +1.7
166,331 - Psyr_0152 0.89 -1.2
166,649 + Psyr_0153 0.58 -0.0
166,649 + Psyr_0153 0.58 -1.9
166,650 - Psyr_0153 0.58 -0.4
166,650 - Psyr_0153 0.58 -1.1
166,734 - Psyr_0153 0.85 -0.2
166,734 - Psyr_0153 0.85 -0.9
166,734 - Psyr_0153 0.85 -0.4
166,784 + -0.2
166,784 + -1.0
167,063 + Psyr_0154 0.16 -1.1
167,064 - Psyr_0154 0.16 -1.1
167,064 - Psyr_0154 0.16 +0.2
167,126 + Psyr_0154 0.19 +0.4
167,126 + Psyr_0154 0.19 -0.2
167,126 + Psyr_0154 0.19 +0.8
167,127 - Psyr_0154 0.19 -0.2
167,127 - Psyr_0154 0.19 +0.3
167,127 - Psyr_0154 0.19 -0.9
167,127 - Psyr_0154 0.19 -0.2
167,127 - Psyr_0154 0.19 +1.0
167,127 - Psyr_0154 0.19 -0.2
167,127 - Psyr_0154 0.19 -0.7
167,127 - Psyr_0154 0.19 -0.2
167,127 - Psyr_0154 0.19 -0.5
167,190 - Psyr_0154 0.22 -0.2
167,254 - Psyr_0154 0.26 +0.7
167,447 + Psyr_0154 0.36 -0.0
167,447 + Psyr_0154 0.36 +0.6
167,447 + Psyr_0154 0.36 -0.8
167,447 + Psyr_0154 0.36 -0.2
167,447 + Psyr_0154 0.36 -0.2
167,447 + Psyr_0154 0.36 +0.5
167,447 + Psyr_0154 0.36 -0.5
167,448 - Psyr_0154 0.36 +0.3
167,448 - Psyr_0154 0.36 -0.4
167,448 - Psyr_0154 0.36 +0.9
167,448 - Psyr_0154 0.36 -0.3
167,448 - Psyr_0154 0.36 +0.5

Or see this region's nucleotide sequence