Experiment: RCH2_defined_Glucose_noNitrogen in EcoFAB_3.5mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS26515 and BWI76_RS26520 overlap by 1 nucleotides BWI76_RS26520 and BWI76_RS26525 are separated by 5 nucleotides BWI76_RS26525 and BWI76_RS26530 are separated by 404 nucleotides
BWI76_RS26515: BWI76_RS26515 - nickel import ATP-binding protein NikD, at 5,488,317 to 5,489,078
_RS26515
BWI76_RS26520: BWI76_RS26520 - nickel import ATP-binding protein NikE, at 5,489,078 to 5,489,848
_RS26520
BWI76_RS26525: BWI76_RS26525 - nickel-responsive transcriptional regulator NikR, at 5,489,854 to 5,490,270
_RS26525
BWI76_RS26530: BWI76_RS26530 - MgtE integral membrane protein, at 5,490,675 to 5,491,688
_RS26530
Position (kb) 5489
5490
5491 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 5488.972 kb on + strand, within BWI76_RS26515 at 5488.980 kb on - strand, within BWI76_RS26515 at 5489.080 kb on - strand at 5489.089 kb on - strand at 5489.140 kb on + strand at 5489.338 kb on - strand, within BWI76_RS26520 at 5489.343 kb on + strand, within BWI76_RS26520 at 5489.401 kb on - strand, within BWI76_RS26520 at 5489.443 kb on - strand, within BWI76_RS26520 at 5489.460 kb on - strand, within BWI76_RS26520 at 5489.493 kb on - strand, within BWI76_RS26520 at 5489.493 kb on - strand, within BWI76_RS26520 at 5489.526 kb on - strand, within BWI76_RS26520 at 5489.875 kb on - strand at 5489.914 kb on - strand, within BWI76_RS26525 at 5489.956 kb on + strand, within BWI76_RS26525 at 5490.001 kb on + strand, within BWI76_RS26525 at 5490.001 kb on + strand, within BWI76_RS26525 at 5490.009 kb on - strand, within BWI76_RS26525 at 5490.009 kb on - strand, within BWI76_RS26525 at 5490.107 kb on + strand, within BWI76_RS26525 at 5490.202 kb on + strand, within BWI76_RS26525 at 5490.264 kb on + strand at 5490.264 kb on + strand at 5490.264 kb on + strand at 5490.266 kb on + strand at 5490.272 kb on - strand at 5490.272 kb on - strand at 5490.575 kb on + strand at 5490.751 kb on - strand at 5490.885 kb on - strand, within BWI76_RS26530 at 5491.020 kb on - strand, within BWI76_RS26530 at 5491.115 kb on + strand, within BWI76_RS26530 at 5491.115 kb on + strand, within BWI76_RS26530 at 5491.123 kb on - strand, within BWI76_RS26530 at 5491.226 kb on - strand, within BWI76_RS26530
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_Glucose_noNitrogen in EcoFAB_3.5mL remove 5,488,972 + BWI76_RS26515 0.86 -0.1 5,488,980 - BWI76_RS26515 0.87 +1.4 5,489,080 - +0.3 5,489,089 - +0.1 5,489,140 + -2.5 5,489,338 - BWI76_RS26520 0.34 +1.6 5,489,343 + BWI76_RS26520 0.34 -0.8 5,489,401 - BWI76_RS26520 0.42 -0.4 5,489,443 - BWI76_RS26520 0.47 +1.3 5,489,460 - BWI76_RS26520 0.50 -1.3 5,489,493 - BWI76_RS26520 0.54 +0.7 5,489,493 - BWI76_RS26520 0.54 -0.0 5,489,526 - BWI76_RS26520 0.58 +3.2 5,489,875 - +2.0 5,489,914 - BWI76_RS26525 0.14 -1.1 5,489,956 + BWI76_RS26525 0.24 -1.0 5,490,001 + BWI76_RS26525 0.35 +0.3 5,490,001 + BWI76_RS26525 0.35 +0.1 5,490,009 - BWI76_RS26525 0.37 +0.6 5,490,009 - BWI76_RS26525 0.37 +0.1 5,490,107 + BWI76_RS26525 0.61 -0.2 5,490,202 + BWI76_RS26525 0.83 +1.7 5,490,264 + +1.8 5,490,264 + -0.1 5,490,264 + -1.6 5,490,266 + +0.8 5,490,272 - -0.3 5,490,272 - +1.0 5,490,575 + +0.6 5,490,751 - -1.9 5,490,885 - BWI76_RS26530 0.21 +1.1 5,491,020 - BWI76_RS26530 0.34 +1.3 5,491,115 + BWI76_RS26530 0.43 -0.7 5,491,115 + BWI76_RS26530 0.43 -0.1 5,491,123 - BWI76_RS26530 0.44 +0.1 5,491,226 - BWI76_RS26530 0.54 +0.1
Or see this region's nucleotide sequence