Experiment: RCH2_defined_Glucose_noNitrogen in EcoFAB_3.5mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS26330 and BWI76_RS26335 are separated by 13 nucleotides BWI76_RS26335 and BWI76_RS26340 overlap by 4 nucleotides BWI76_RS26340 and BWI76_RS26345 overlap by 4 nucleotides BWI76_RS26345 and BWI76_RS26350 are separated by 60 nucleotides
BWI76_RS26330: BWI76_RS26330 - ABC transporter ATP-binding protein, at 5,450,614 to 5,451,315
_RS26330
BWI76_RS26335: BWI76_RS26335 - ABC transporter ATP-binding protein, at 5,451,329 to 5,452,096
_RS26335
BWI76_RS26340: BWI76_RS26340 - branched-chain amino acid ABC transporter permease, at 5,452,093 to 5,453,373
_RS26340
BWI76_RS26345: BWI76_RS26345 - branched-chain amino acid ABC transporter permease LivH, at 5,453,370 to 5,454,296
_RS26345
BWI76_RS26350: BWI76_RS26350 - branched chain amino acid ABC transporter substrate-binding protein, at 5,454,357 to 5,455,466
_RS26350
Position (kb) 5452
5453
5454 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 5451.133 kb on + strand, within BWI76_RS26330 at 5451.230 kb on + strand, within BWI76_RS26330 at 5451.290 kb on + strand at 5451.407 kb on + strand, within BWI76_RS26335 at 5451.472 kb on + strand, within BWI76_RS26335 at 5451.591 kb on + strand, within BWI76_RS26335 at 5451.738 kb on - strand, within BWI76_RS26335 at 5452.303 kb on - strand, within BWI76_RS26340 at 5452.311 kb on - strand, within BWI76_RS26340 at 5452.353 kb on - strand, within BWI76_RS26340 at 5452.518 kb on - strand, within BWI76_RS26340 at 5452.548 kb on + strand at 5452.574 kb on + strand, within BWI76_RS26340 at 5452.712 kb on - strand, within BWI76_RS26340 at 5452.712 kb on - strand, within BWI76_RS26340 at 5452.844 kb on + strand, within BWI76_RS26340 at 5452.885 kb on - strand, within BWI76_RS26340 at 5452.951 kb on + strand, within BWI76_RS26340 at 5452.992 kb on + strand, within BWI76_RS26340 at 5453.083 kb on - strand, within BWI76_RS26340 at 5453.341 kb on + strand at 5453.394 kb on + strand at 5453.556 kb on - strand, within BWI76_RS26345 at 5453.622 kb on - strand, within BWI76_RS26345 at 5453.889 kb on - strand, within BWI76_RS26345 at 5453.955 kb on + strand, within BWI76_RS26345 at 5453.973 kb on - strand, within BWI76_RS26345 at 5454.161 kb on - strand, within BWI76_RS26345 at 5454.243 kb on - strand at 5454.253 kb on - strand at 5454.292 kb on + strand at 5454.292 kb on + strand at 5454.370 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_Glucose_noNitrogen in EcoFAB_3.5mL remove 5,451,133 + BWI76_RS26330 0.74 +0.3 5,451,230 + BWI76_RS26330 0.88 -2.5 5,451,290 + -0.6 5,451,407 + BWI76_RS26335 0.10 -2.0 5,451,472 + BWI76_RS26335 0.19 +0.5 5,451,591 + BWI76_RS26335 0.34 +0.4 5,451,738 - BWI76_RS26335 0.53 +2.8 5,452,303 - BWI76_RS26340 0.16 +0.2 5,452,311 - BWI76_RS26340 0.17 +0.8 5,452,353 - BWI76_RS26340 0.20 +0.8 5,452,518 - BWI76_RS26340 0.33 -0.0 5,452,548 + -0.4 5,452,574 + BWI76_RS26340 0.38 -0.5 5,452,712 - BWI76_RS26340 0.48 +0.7 5,452,712 - BWI76_RS26340 0.48 +0.6 5,452,844 + BWI76_RS26340 0.59 +1.3 5,452,885 - BWI76_RS26340 0.62 +0.1 5,452,951 + BWI76_RS26340 0.67 +0.5 5,452,992 + BWI76_RS26340 0.70 +0.8 5,453,083 - BWI76_RS26340 0.77 +0.4 5,453,341 + -1.6 5,453,394 + +1.5 5,453,556 - BWI76_RS26345 0.20 -0.5 5,453,622 - BWI76_RS26345 0.27 -0.2 5,453,889 - BWI76_RS26345 0.56 +0.8 5,453,955 + BWI76_RS26345 0.63 -0.2 5,453,973 - BWI76_RS26345 0.65 +0.8 5,454,161 - BWI76_RS26345 0.85 +3.2 5,454,243 - -0.4 5,454,253 - -0.2 5,454,292 + +1.6 5,454,292 + -1.1 5,454,370 + -1.0
Or see this region's nucleotide sequence