Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS23995

Experiment: RCH2_defined_Glucose_noNitrogen in EcoFAB_3.5mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS23985 and BWI76_RS23990 are separated by 377 nucleotidesBWI76_RS23990 and BWI76_RS23995 are separated by 24 nucleotidesBWI76_RS23995 and BWI76_RS24000 are separated by 0 nucleotidesBWI76_RS24000 and BWI76_RS24005 overlap by 13 nucleotides BWI76_RS23985: BWI76_RS23985 - erythrose-4-phosphate dehydrogenase, at 5,006,463 to 5,007,482 _RS23985 BWI76_RS23990: BWI76_RS23990 - DNA-binding protein, at 5,007,860 to 5,008,285 _RS23990 BWI76_RS23995: BWI76_RS23995 - hypothetical protein, at 5,008,310 to 5,008,888 _RS23995 BWI76_RS24000: BWI76_RS24000 - hypothetical protein, at 5,008,889 to 5,009,596 _RS24000 BWI76_RS24005: BWI76_RS24005 - cobalt ABC transporter ATP-binding protein, at 5,009,584 to 5,010,261 _RS24005 Position (kb) 5008 5009Strain fitness (log2 ratio) -1 0 1 2 3 4at 5007.371 kb on + strand, within BWI76_RS23985at 5007.458 kb on - strandat 5007.490 kb on + strandat 5007.490 kb on + strandat 5007.524 kb on + strandat 5007.532 kb on - strandat 5007.532 kb on - strandat 5007.539 kb on + strandat 5007.547 kb on - strandat 5007.625 kb on - strandat 5007.875 kb on + strandat 5007.897 kb on + strandat 5007.902 kb on - strandat 5007.958 kb on + strand, within BWI76_RS23990at 5008.024 kb on - strand, within BWI76_RS23990at 5008.155 kb on - strand, within BWI76_RS23990at 5008.170 kb on + strand, within BWI76_RS23990at 5008.178 kb on - strand, within BWI76_RS23990at 5008.188 kb on + strand, within BWI76_RS23990at 5008.196 kb on - strand, within BWI76_RS23990at 5008.756 kb on - strand, within BWI76_RS23995at 5008.877 kb on + strandat 5008.913 kb on - strandat 5008.932 kb on + strandat 5008.950 kb on - strandat 5008.961 kb on + strand, within BWI76_RS24000at 5008.961 kb on + strand, within BWI76_RS24000at 5008.970 kb on - strand, within BWI76_RS24000at 5008.977 kb on - strand, within BWI76_RS24000at 5008.984 kb on + strand, within BWI76_RS24000at 5008.984 kb on + strand, within BWI76_RS24000at 5009.061 kb on + strand, within BWI76_RS24000at 5009.069 kb on - strand, within BWI76_RS24000at 5009.284 kb on + strand, within BWI76_RS24000at 5009.310 kb on - strand, within BWI76_RS24000at 5009.415 kb on + strand, within BWI76_RS24000at 5009.525 kb on - strand, within BWI76_RS24000at 5009.528 kb on - strandat 5009.591 kb on + strandat 5009.591 kb on + strandat 5009.721 kb on + strand, within BWI76_RS24005at 5009.729 kb on - strand, within BWI76_RS24005at 5009.828 kb on - strand, within BWI76_RS24005at 5009.828 kb on - strand, within BWI76_RS24005

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_Glucose_noNitrogen in EcoFAB_3.5mL
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5,007,371 + BWI76_RS23985 0.89 -0.4
5,007,458 - -1.1
5,007,490 + +0.4
5,007,490 + -0.1
5,007,524 + +0.0
5,007,532 - +0.1
5,007,532 - -1.6
5,007,539 + +2.0
5,007,547 - -0.8
5,007,625 - -0.0
5,007,875 + -0.5
5,007,897 + -0.1
5,007,902 - -0.5
5,007,958 + BWI76_RS23990 0.23 +0.7
5,008,024 - BWI76_RS23990 0.38 +0.5
5,008,155 - BWI76_RS23990 0.69 -0.3
5,008,170 + BWI76_RS23990 0.73 +0.1
5,008,178 - BWI76_RS23990 0.75 +0.5
5,008,188 + BWI76_RS23990 0.77 +0.0
5,008,196 - BWI76_RS23990 0.79 +1.7
5,008,756 - BWI76_RS23995 0.77 -0.7
5,008,877 + +0.7
5,008,913 - +0.0
5,008,932 + -1.1
5,008,950 - -0.4
5,008,961 + BWI76_RS24000 0.10 -0.5
5,008,961 + BWI76_RS24000 0.10 +0.2
5,008,970 - BWI76_RS24000 0.11 -0.5
5,008,977 - BWI76_RS24000 0.12 +2.5
5,008,984 + BWI76_RS24000 0.13 -0.7
5,008,984 + BWI76_RS24000 0.13 -0.3
5,009,061 + BWI76_RS24000 0.24 -0.3
5,009,069 - BWI76_RS24000 0.25 +0.8
5,009,284 + BWI76_RS24000 0.56 +0.6
5,009,310 - BWI76_RS24000 0.59 +0.3
5,009,415 + BWI76_RS24000 0.74 -0.8
5,009,525 - BWI76_RS24000 0.90 -0.9
5,009,528 - -0.6
5,009,591 + +0.5
5,009,591 + +0.8
5,009,721 + BWI76_RS24005 0.20 +1.5
5,009,729 - BWI76_RS24005 0.21 +0.2
5,009,828 - BWI76_RS24005 0.36 +0.0
5,009,828 - BWI76_RS24005 0.36 +4.1

Or see this region's nucleotide sequence