Experiment: RCH2_defined_Glucose_noNitrogen in EcoFAB_3.5mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS23985 and BWI76_RS23990 are separated by 377 nucleotides BWI76_RS23990 and BWI76_RS23995 are separated by 24 nucleotides BWI76_RS23995 and BWI76_RS24000 are separated by 0 nucleotides BWI76_RS24000 and BWI76_RS24005 overlap by 13 nucleotides
BWI76_RS23985: BWI76_RS23985 - erythrose-4-phosphate dehydrogenase, at 5,006,463 to 5,007,482
_RS23985
BWI76_RS23990: BWI76_RS23990 - DNA-binding protein, at 5,007,860 to 5,008,285
_RS23990
BWI76_RS23995: BWI76_RS23995 - hypothetical protein, at 5,008,310 to 5,008,888
_RS23995
BWI76_RS24000: BWI76_RS24000 - hypothetical protein, at 5,008,889 to 5,009,596
_RS24000
BWI76_RS24005: BWI76_RS24005 - cobalt ABC transporter ATP-binding protein, at 5,009,584 to 5,010,261
_RS24005
Position (kb)
5008
5009 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 5007.371 kb on + strand, within BWI76_RS23985 at 5007.458 kb on - strand at 5007.490 kb on + strand at 5007.490 kb on + strand at 5007.524 kb on + strand at 5007.532 kb on - strand at 5007.532 kb on - strand at 5007.539 kb on + strand at 5007.547 kb on - strand at 5007.625 kb on - strand at 5007.875 kb on + strand at 5007.897 kb on + strand at 5007.902 kb on - strand at 5007.958 kb on + strand, within BWI76_RS23990 at 5008.024 kb on - strand, within BWI76_RS23990 at 5008.155 kb on - strand, within BWI76_RS23990 at 5008.170 kb on + strand, within BWI76_RS23990 at 5008.178 kb on - strand, within BWI76_RS23990 at 5008.188 kb on + strand, within BWI76_RS23990 at 5008.196 kb on - strand, within BWI76_RS23990 at 5008.756 kb on - strand, within BWI76_RS23995 at 5008.877 kb on + strand at 5008.913 kb on - strand at 5008.932 kb on + strand at 5008.950 kb on - strand at 5008.961 kb on + strand, within BWI76_RS24000 at 5008.961 kb on + strand, within BWI76_RS24000 at 5008.970 kb on - strand, within BWI76_RS24000 at 5008.977 kb on - strand, within BWI76_RS24000 at 5008.984 kb on + strand, within BWI76_RS24000 at 5008.984 kb on + strand, within BWI76_RS24000 at 5009.061 kb on + strand, within BWI76_RS24000 at 5009.069 kb on - strand, within BWI76_RS24000 at 5009.284 kb on + strand, within BWI76_RS24000 at 5009.310 kb on - strand, within BWI76_RS24000 at 5009.415 kb on + strand, within BWI76_RS24000 at 5009.525 kb on - strand, within BWI76_RS24000 at 5009.528 kb on - strand at 5009.591 kb on + strand at 5009.591 kb on + strand at 5009.721 kb on + strand, within BWI76_RS24005 at 5009.729 kb on - strand, within BWI76_RS24005 at 5009.828 kb on - strand, within BWI76_RS24005 at 5009.828 kb on - strand, within BWI76_RS24005
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_Glucose_noNitrogen in EcoFAB_3.5mL remove 5,007,371 + BWI76_RS23985 0.89 -0.4 5,007,458 - -1.1 5,007,490 + +0.4 5,007,490 + -0.1 5,007,524 + +0.0 5,007,532 - +0.1 5,007,532 - -1.6 5,007,539 + +2.0 5,007,547 - -0.8 5,007,625 - -0.0 5,007,875 + -0.5 5,007,897 + -0.1 5,007,902 - -0.5 5,007,958 + BWI76_RS23990 0.23 +0.7 5,008,024 - BWI76_RS23990 0.38 +0.5 5,008,155 - BWI76_RS23990 0.69 -0.3 5,008,170 + BWI76_RS23990 0.73 +0.1 5,008,178 - BWI76_RS23990 0.75 +0.5 5,008,188 + BWI76_RS23990 0.77 +0.0 5,008,196 - BWI76_RS23990 0.79 +1.7 5,008,756 - BWI76_RS23995 0.77 -0.7 5,008,877 + +0.7 5,008,913 - +0.0 5,008,932 + -1.1 5,008,950 - -0.4 5,008,961 + BWI76_RS24000 0.10 -0.5 5,008,961 + BWI76_RS24000 0.10 +0.2 5,008,970 - BWI76_RS24000 0.11 -0.5 5,008,977 - BWI76_RS24000 0.12 +2.5 5,008,984 + BWI76_RS24000 0.13 -0.7 5,008,984 + BWI76_RS24000 0.13 -0.3 5,009,061 + BWI76_RS24000 0.24 -0.3 5,009,069 - BWI76_RS24000 0.25 +0.8 5,009,284 + BWI76_RS24000 0.56 +0.6 5,009,310 - BWI76_RS24000 0.59 +0.3 5,009,415 + BWI76_RS24000 0.74 -0.8 5,009,525 - BWI76_RS24000 0.90 -0.9 5,009,528 - -0.6 5,009,591 + +0.5 5,009,591 + +0.8 5,009,721 + BWI76_RS24005 0.20 +1.5 5,009,729 - BWI76_RS24005 0.21 +0.2 5,009,828 - BWI76_RS24005 0.36 +0.0 5,009,828 - BWI76_RS24005 0.36 +4.1
Or see this region's nucleotide sequence