Experiment: RCH2_defined_Glucose_noNitrogen in EcoFAB_3.5mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS22465 and BWI76_RS22470 overlap by 1 nucleotides BWI76_RS22470 and BWI76_RS22475 overlap by 8 nucleotides BWI76_RS22475 and BWI76_RS22480 are separated by 96 nucleotides
BWI76_RS22465: BWI76_RS22465 - hemin ABC transporter substrate-binding protein, at 4,684,110 to 4,684,928
_RS22465
BWI76_RS22470: BWI76_RS22470 - transporter permease, at 4,684,928 to 4,685,923
_RS22470
BWI76_RS22475: BWI76_RS22475 - heme ABC transporter ATP-binding protein, at 4,685,916 to 4,686,695
_RS22475
BWI76_RS22480: BWI76_RS22480 - lactaldehyde reductase, at 4,686,792 to 4,687,943
_RS22480
Position (kb) 4685
4686
4687 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4685.003 kb on - strand at 4685.172 kb on - strand, within BWI76_RS22470 at 4685.404 kb on + strand, within BWI76_RS22470 at 4685.513 kb on + strand, within BWI76_RS22470 at 4685.798 kb on + strand, within BWI76_RS22470 at 4686.110 kb on - strand, within BWI76_RS22475 at 4686.228 kb on + strand, within BWI76_RS22475 at 4686.468 kb on + strand, within BWI76_RS22475 at 4686.530 kb on + strand, within BWI76_RS22475 at 4686.576 kb on + strand, within BWI76_RS22475 at 4686.638 kb on + strand at 4686.654 kb on + strand at 4686.682 kb on + strand at 4686.685 kb on - strand at 4686.690 kb on - strand at 4687.067 kb on - strand, within BWI76_RS22480 at 4687.102 kb on + strand, within BWI76_RS22480 at 4687.102 kb on + strand, within BWI76_RS22480 at 4687.148 kb on + strand, within BWI76_RS22480 at 4687.175 kb on - strand, within BWI76_RS22480 at 4687.214 kb on + strand, within BWI76_RS22480 at 4687.318 kb on - strand, within BWI76_RS22480 at 4687.350 kb on + strand, within BWI76_RS22480 at 4687.478 kb on - strand, within BWI76_RS22480 at 4687.583 kb on - strand, within BWI76_RS22480 at 4687.631 kb on + strand, within BWI76_RS22480 at 4687.631 kb on + strand, within BWI76_RS22480 at 4687.639 kb on - strand, within BWI76_RS22480 at 4687.639 kb on - strand, within BWI76_RS22480 at 4687.641 kb on + strand, within BWI76_RS22480 at 4687.644 kb on - strand, within BWI76_RS22480 at 4687.654 kb on - strand, within BWI76_RS22480 at 4687.691 kb on + strand, within BWI76_RS22480 at 4687.691 kb on + strand, within BWI76_RS22480 at 4687.691 kb on + strand, within BWI76_RS22480
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_Glucose_noNitrogen in EcoFAB_3.5mL remove 4,685,003 - -0.7 4,685,172 - BWI76_RS22470 0.24 +0.7 4,685,404 + BWI76_RS22470 0.48 -0.2 4,685,513 + BWI76_RS22470 0.59 +2.7 4,685,798 + BWI76_RS22470 0.87 -0.1 4,686,110 - BWI76_RS22475 0.25 -3.7 4,686,228 + BWI76_RS22475 0.40 +0.4 4,686,468 + BWI76_RS22475 0.71 -1.9 4,686,530 + BWI76_RS22475 0.79 -0.6 4,686,576 + BWI76_RS22475 0.85 +0.8 4,686,638 + -1.6 4,686,654 + +0.3 4,686,682 + +1.4 4,686,685 - +0.8 4,686,690 - +0.4 4,687,067 - BWI76_RS22480 0.24 +1.0 4,687,102 + BWI76_RS22480 0.27 +0.9 4,687,102 + BWI76_RS22480 0.27 -0.3 4,687,148 + BWI76_RS22480 0.31 +0.9 4,687,175 - BWI76_RS22480 0.33 +0.2 4,687,214 + BWI76_RS22480 0.37 -3.3 4,687,318 - BWI76_RS22480 0.46 +1.0 4,687,350 + BWI76_RS22480 0.48 -0.4 4,687,478 - BWI76_RS22480 0.60 -0.6 4,687,583 - BWI76_RS22480 0.69 -0.2 4,687,631 + BWI76_RS22480 0.73 -0.0 4,687,631 + BWI76_RS22480 0.73 -0.2 4,687,639 - BWI76_RS22480 0.74 -0.0 4,687,639 - BWI76_RS22480 0.74 -2.3 4,687,641 + BWI76_RS22480 0.74 +1.0 4,687,644 - BWI76_RS22480 0.74 +0.4 4,687,654 - BWI76_RS22480 0.75 -1.8 4,687,691 + BWI76_RS22480 0.78 -0.4 4,687,691 + BWI76_RS22480 0.78 -0.8 4,687,691 + BWI76_RS22480 0.78 +0.1
Or see this region's nucleotide sequence