Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS22270

Experiment: RCH2_defined_Glucose_noNitrogen in EcoFAB_3.5mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS22265 and BWI76_RS22270 are separated by 254 nucleotidesBWI76_RS22270 and BWI76_RS22275 are separated by 11 nucleotides BWI76_RS22265: BWI76_RS22265 - NADH:flavorubredoxin oxidoreductase, at 4,640,264 to 4,641,397 _RS22265 BWI76_RS22270: BWI76_RS22270 - nickel transporter, at 4,641,652 to 4,642,665 _RS22270 BWI76_RS22275: BWI76_RS22275 - carbamoyltransferase HypF, at 4,642,677 to 4,644,896 _RS22275 Position (kb) 4641 4642 4643Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 4640.663 kb on + strand, within BWI76_RS22265at 4640.894 kb on - strand, within BWI76_RS22265at 4641.366 kb on + strandat 4641.391 kb on - strandat 4641.528 kb on - strandat 4641.651 kb on - strandat 4641.721 kb on - strandat 4641.776 kb on - strand, within BWI76_RS22270at 4641.839 kb on + strand, within BWI76_RS22270at 4641.847 kb on - strand, within BWI76_RS22270at 4641.893 kb on + strand, within BWI76_RS22270at 4641.930 kb on - strand, within BWI76_RS22270at 4642.218 kb on + strand, within BWI76_RS22270at 4642.226 kb on - strand, within BWI76_RS22270at 4642.582 kb on + strandat 4642.590 kb on - strandat 4642.671 kb on - strandat 4642.674 kb on + strandat 4642.682 kb on - strandat 4642.944 kb on + strand, within BWI76_RS22275at 4642.973 kb on + strand, within BWI76_RS22275at 4643.190 kb on + strand, within BWI76_RS22275at 4643.292 kb on - strand, within BWI76_RS22275at 4643.427 kb on - strand, within BWI76_RS22275at 4643.592 kb on - strand, within BWI76_RS22275at 4643.607 kb on - strand, within BWI76_RS22275

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_Glucose_noNitrogen in EcoFAB_3.5mL
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4,640,663 + BWI76_RS22265 0.35 -0.9
4,640,894 - BWI76_RS22265 0.56 -0.5
4,641,366 + +1.0
4,641,391 - -0.7
4,641,528 - -0.2
4,641,651 - +0.2
4,641,721 - +1.0
4,641,776 - BWI76_RS22270 0.12 -0.9
4,641,839 + BWI76_RS22270 0.18 -1.8
4,641,847 - BWI76_RS22270 0.19 -0.6
4,641,893 + BWI76_RS22270 0.24 +0.9
4,641,930 - BWI76_RS22270 0.27 +1.0
4,642,218 + BWI76_RS22270 0.56 +4.7
4,642,226 - BWI76_RS22270 0.57 +0.7
4,642,582 + +1.0
4,642,590 - -1.5
4,642,671 - +0.3
4,642,674 + -2.4
4,642,682 - -0.3
4,642,944 + BWI76_RS22275 0.12 +0.6
4,642,973 + BWI76_RS22275 0.13 -0.6
4,643,190 + BWI76_RS22275 0.23 -0.1
4,643,292 - BWI76_RS22275 0.28 -0.4
4,643,427 - BWI76_RS22275 0.34 -0.9
4,643,592 - BWI76_RS22275 0.41 +0.3
4,643,607 - BWI76_RS22275 0.42 -0.9

Or see this region's nucleotide sequence