Experiment: RCH2_defined_Glucose_noNitrogen in EcoFAB_3.5mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS21925 and BWI76_RS21930 are separated by 134 nucleotides BWI76_RS21930 and BWI76_RS21935 overlap by 4 nucleotides
BWI76_RS21925: BWI76_RS21925 - agmatinase, at 4,580,304 to 4,581,275
_RS21925
BWI76_RS21930: BWI76_RS21930 - LysR family transcriptional regulator, at 4,581,410 to 4,582,312
_RS21930
BWI76_RS21935: BWI76_RS21935 - MFS transporter, at 4,582,309 to 4,583,502
_RS21935
Position (kb)
4581
4582
4583 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4580.595 kb on + strand, within BWI76_RS21925 at 4580.630 kb on + strand, within BWI76_RS21925 at 4580.673 kb on + strand, within BWI76_RS21925 at 4580.673 kb on - strand, within BWI76_RS21925 at 4580.998 kb on + strand, within BWI76_RS21925 at 4581.154 kb on + strand, within BWI76_RS21925 at 4581.159 kb on + strand, within BWI76_RS21925 at 4581.167 kb on - strand, within BWI76_RS21925 at 4581.284 kb on + strand at 4581.284 kb on + strand at 4581.284 kb on + strand at 4581.292 kb on - strand at 4581.404 kb on - strand at 4581.483 kb on - strand at 4581.506 kb on - strand, within BWI76_RS21930 at 4581.723 kb on + strand, within BWI76_RS21930 at 4581.811 kb on + strand at 4581.947 kb on + strand, within BWI76_RS21930 at 4582.053 kb on + strand, within BWI76_RS21930 at 4582.101 kb on + strand, within BWI76_RS21930 at 4582.148 kb on + strand, within BWI76_RS21930 at 4582.175 kb on - strand, within BWI76_RS21930 at 4582.253 kb on + strand at 4582.280 kb on - strand at 4582.771 kb on - strand, within BWI76_RS21935 at 4582.831 kb on + strand, within BWI76_RS21935 at 4582.831 kb on + strand, within BWI76_RS21935 at 4582.986 kb on - strand, within BWI76_RS21935 at 4583.084 kb on + strand, within BWI76_RS21935 at 4583.227 kb on + strand, within BWI76_RS21935
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_Glucose_noNitrogen in EcoFAB_3.5mL remove 4,580,595 + BWI76_RS21925 0.30 +0.2 4,580,630 + BWI76_RS21925 0.34 +0.1 4,580,673 + BWI76_RS21925 0.38 -0.4 4,580,673 - BWI76_RS21925 0.38 +1.3 4,580,998 + BWI76_RS21925 0.71 -0.1 4,581,154 + BWI76_RS21925 0.87 +0.3 4,581,159 + BWI76_RS21925 0.88 +1.9 4,581,167 - BWI76_RS21925 0.89 +0.2 4,581,284 + -0.8 4,581,284 + -1.1 4,581,284 + -0.3 4,581,292 - -2.4 4,581,404 - +0.0 4,581,483 - +1.6 4,581,506 - BWI76_RS21930 0.11 +2.2 4,581,723 + BWI76_RS21930 0.35 +0.6 4,581,811 + +1.4 4,581,947 + BWI76_RS21930 0.59 +1.1 4,582,053 + BWI76_RS21930 0.71 +1.3 4,582,101 + BWI76_RS21930 0.77 +1.6 4,582,148 + BWI76_RS21930 0.82 +0.6 4,582,175 - BWI76_RS21930 0.85 -0.8 4,582,253 + +1.4 4,582,280 - -0.1 4,582,771 - BWI76_RS21935 0.39 +0.4 4,582,831 + BWI76_RS21935 0.44 -1.4 4,582,831 + BWI76_RS21935 0.44 +2.4 4,582,986 - BWI76_RS21935 0.57 +0.8 4,583,084 + BWI76_RS21935 0.65 -0.1 4,583,227 + BWI76_RS21935 0.77 -2.4
Or see this region's nucleotide sequence