Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS20855

Experiment: RCH2_defined_Glucose_noNitrogen in EcoFAB_3.5mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS20850 and BWI76_RS20855 are separated by 186 nucleotidesBWI76_RS20855 and BWI76_RS20860 are separated by 5 nucleotides BWI76_RS20850: BWI76_RS20850 - oxidoreductase FeS-binding subunit, at 4,357,835 to 4,359,799 _RS20850 BWI76_RS20855: BWI76_RS20855 - hypothetical protein, at 4,359,986 to 4,360,166 _RS20855 BWI76_RS20860: BWI76_RS20860 - aminoglycoside/multidrug transporter subunit AcrD, at 4,360,172 to 4,363,285 _RS20860 Position (kb) 4359 4360 4361Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4 5 6 7 8 9at 4359.006 kb on + strand, within BWI76_RS20850at 4359.093 kb on + strand, within BWI76_RS20850at 4359.150 kb on + strand, within BWI76_RS20850at 4359.155 kb on + strand, within BWI76_RS20850at 4359.321 kb on + strand, within BWI76_RS20850at 4359.350 kb on - strand, within BWI76_RS20850at 4359.636 kb on - strandat 4359.668 kb on - strandat 4359.670 kb on + strandat 4359.742 kb on + strandat 4359.983 kb on + strandat 4360.001 kb on + strandat 4360.009 kb on - strand, within BWI76_RS20855at 4360.011 kb on + strand, within BWI76_RS20855at 4360.013 kb on + strand, within BWI76_RS20855at 4360.025 kb on + strand, within BWI76_RS20855at 4360.033 kb on + strand, within BWI76_RS20855at 4360.098 kb on + strand, within BWI76_RS20855at 4360.100 kb on - strand, within BWI76_RS20855at 4360.106 kb on - strand, within BWI76_RS20855at 4360.106 kb on - strand, within BWI76_RS20855at 4360.118 kb on + strand, within BWI76_RS20855at 4360.120 kb on + strand, within BWI76_RS20855at 4360.120 kb on + strand, within BWI76_RS20855at 4360.120 kb on + strand, within BWI76_RS20855at 4360.128 kb on - strand, within BWI76_RS20855at 4360.235 kb on + strandat 4360.275 kb on + strandat 4360.367 kb on + strandat 4360.372 kb on + strandat 4360.417 kb on - strandat 4360.471 kb on + strandat 4360.525 kb on + strand, within BWI76_RS20860at 4360.583 kb on - strand, within BWI76_RS20860at 4360.707 kb on - strand, within BWI76_RS20860at 4360.747 kb on + strand, within BWI76_RS20860at 4360.825 kb on + strandat 4360.909 kb on - strand, within BWI76_RS20860at 4360.963 kb on + strand, within BWI76_RS20860at 4360.981 kb on - strand, within BWI76_RS20860at 4361.048 kb on - strand, within BWI76_RS20860at 4361.119 kb on - strand, within BWI76_RS20860

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_Glucose_noNitrogen in EcoFAB_3.5mL
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4,359,006 + BWI76_RS20850 0.60 -0.3
4,359,093 + BWI76_RS20850 0.64 -0.1
4,359,150 + BWI76_RS20850 0.67 -0.4
4,359,155 + BWI76_RS20850 0.67 +0.3
4,359,321 + BWI76_RS20850 0.76 -2.9
4,359,350 - BWI76_RS20850 0.77 +0.7
4,359,636 - -1.9
4,359,668 - +0.8
4,359,670 + +1.6
4,359,742 + +1.0
4,359,983 + +0.2
4,360,001 + -0.6
4,360,009 - BWI76_RS20855 0.13 -0.1
4,360,011 + BWI76_RS20855 0.14 +0.6
4,360,013 + BWI76_RS20855 0.15 -1.8
4,360,025 + BWI76_RS20855 0.22 +0.8
4,360,033 + BWI76_RS20855 0.26 -0.1
4,360,098 + BWI76_RS20855 0.62 +0.0
4,360,100 - BWI76_RS20855 0.63 +0.4
4,360,106 - BWI76_RS20855 0.66 -0.3
4,360,106 - BWI76_RS20855 0.66 +0.6
4,360,118 + BWI76_RS20855 0.73 -0.2
4,360,120 + BWI76_RS20855 0.74 -0.8
4,360,120 + BWI76_RS20855 0.74 +0.6
4,360,120 + BWI76_RS20855 0.74 +9.5
4,360,128 - BWI76_RS20855 0.78 +0.4
4,360,235 + -0.8
4,360,275 + +0.8
4,360,367 + +0.5
4,360,372 + +1.0
4,360,417 - -1.2
4,360,471 + -0.0
4,360,525 + BWI76_RS20860 0.11 +0.4
4,360,583 - BWI76_RS20860 0.13 -0.9
4,360,707 - BWI76_RS20860 0.17 +0.9
4,360,747 + BWI76_RS20860 0.18 -0.9
4,360,825 + +1.4
4,360,909 - BWI76_RS20860 0.24 -0.7
4,360,963 + BWI76_RS20860 0.25 +0.6
4,360,981 - BWI76_RS20860 0.26 -0.2
4,361,048 - BWI76_RS20860 0.28 +0.2
4,361,119 - BWI76_RS20860 0.30 -1.4

Or see this region's nucleotide sequence