Experiment: RCH2_defined_Glucose_noNitrogen in EcoFAB_3.5mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16585 and BWI76_RS16590 overlap by 4 nucleotides BWI76_RS16590 and BWI76_RS16595 are separated by 24 nucleotides BWI76_RS16595 and BWI76_RS16600 are separated by 12 nucleotides
BWI76_RS16585: BWI76_RS16585 - trehalose synthase, at 3,435,057 to 3,436,682
_RS16585
BWI76_RS16590: BWI76_RS16590 - short-chain dehydrogenase, at 3,436,679 to 3,437,668
_RS16590
BWI76_RS16595: BWI76_RS16595 - hypothetical protein, at 3,437,693 to 3,438,211
_RS16595
BWI76_RS16600: BWI76_RS16600 - DUF421 domain-containing protein, at 3,438,224 to 3,438,670
_RS16600
Position (kb)
3436
3437
3438 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3435.757 kb on - strand, within BWI76_RS16585 at 3435.930 kb on + strand, within BWI76_RS16585 at 3436.004 kb on - strand, within BWI76_RS16585 at 3436.112 kb on + strand, within BWI76_RS16585 at 3436.565 kb on - strand at 3436.971 kb on - strand, within BWI76_RS16590 at 3437.313 kb on + strand, within BWI76_RS16590 at 3437.617 kb on - strand at 3437.879 kb on - strand, within BWI76_RS16595 at 3437.913 kb on + strand, within BWI76_RS16595 at 3437.931 kb on + strand, within BWI76_RS16595 at 3438.022 kb on + strand, within BWI76_RS16595 at 3438.022 kb on + strand, within BWI76_RS16595 at 3438.091 kb on + strand, within BWI76_RS16595 at 3438.091 kb on + strand, within BWI76_RS16595 at 3438.099 kb on - strand, within BWI76_RS16595 at 3438.110 kb on - strand, within BWI76_RS16595 at 3438.191 kb on + strand at 3438.198 kb on - strand at 3438.212 kb on + strand at 3438.256 kb on - strand at 3438.266 kb on - strand at 3438.266 kb on - strand at 3438.302 kb on + strand, within BWI76_RS16600 at 3438.313 kb on + strand, within BWI76_RS16600 at 3438.349 kb on - strand, within BWI76_RS16600 at 3438.393 kb on + strand, within BWI76_RS16600 at 3438.400 kb on - strand, within BWI76_RS16600 at 3438.474 kb on + strand, within BWI76_RS16600 at 3438.493 kb on + strand, within BWI76_RS16600 at 3438.493 kb on + strand, within BWI76_RS16600 at 3438.493 kb on + strand, within BWI76_RS16600 at 3438.493 kb on + strand, within BWI76_RS16600 at 3438.494 kb on + strand, within BWI76_RS16600 at 3438.501 kb on - strand, within BWI76_RS16600 at 3438.536 kb on + strand, within BWI76_RS16600 at 3438.571 kb on + strand at 3438.571 kb on + strand, within BWI76_RS16600 at 3438.571 kb on + strand, within BWI76_RS16600 at 3438.571 kb on + strand, within BWI76_RS16600 at 3438.571 kb on + strand, within BWI76_RS16600 at 3438.578 kb on + strand, within BWI76_RS16600 at 3438.579 kb on - strand, within BWI76_RS16600 at 3438.579 kb on - strand, within BWI76_RS16600 at 3438.579 kb on - strand, within BWI76_RS16600 at 3438.579 kb on - strand, within BWI76_RS16600 at 3438.579 kb on - strand, within BWI76_RS16600 at 3438.579 kb on - strand, within BWI76_RS16600 at 3438.579 kb on - strand, within BWI76_RS16600 at 3438.579 kb on - strand, within BWI76_RS16600 at 3438.579 kb on - strand, within BWI76_RS16600 at 3438.579 kb on - strand, within BWI76_RS16600 at 3438.579 kb on - strand, within BWI76_RS16600 at 3438.579 kb on - strand, within BWI76_RS16600 at 3438.593 kb on - strand, within BWI76_RS16600 at 3438.668 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_Glucose_noNitrogen in EcoFAB_3.5mL remove 3,435,757 - BWI76_RS16585 0.43 -0.6 3,435,930 + BWI76_RS16585 0.54 +0.1 3,436,004 - BWI76_RS16585 0.58 -1.8 3,436,112 + BWI76_RS16585 0.65 -0.4 3,436,565 - -0.5 3,436,971 - BWI76_RS16590 0.29 -0.8 3,437,313 + BWI76_RS16590 0.64 +0.7 3,437,617 - -0.2 3,437,879 - BWI76_RS16595 0.36 +0.3 3,437,913 + BWI76_RS16595 0.42 +1.2 3,437,931 + BWI76_RS16595 0.46 -0.3 3,438,022 + BWI76_RS16595 0.63 +0.8 3,438,022 + BWI76_RS16595 0.63 +0.2 3,438,091 + BWI76_RS16595 0.77 -1.6 3,438,091 + BWI76_RS16595 0.77 -0.0 3,438,099 - BWI76_RS16595 0.78 +0.2 3,438,110 - BWI76_RS16595 0.80 +0.2 3,438,191 + -0.9 3,438,198 - +1.2 3,438,212 + +0.5 3,438,256 - -0.3 3,438,266 - +0.1 3,438,266 - +1.0 3,438,302 + BWI76_RS16600 0.17 +0.2 3,438,313 + BWI76_RS16600 0.20 -1.1 3,438,349 - BWI76_RS16600 0.28 -1.0 3,438,393 + BWI76_RS16600 0.38 -1.1 3,438,400 - BWI76_RS16600 0.39 -0.3 3,438,474 + BWI76_RS16600 0.56 -0.9 3,438,493 + BWI76_RS16600 0.60 -1.9 3,438,493 + BWI76_RS16600 0.60 -0.8 3,438,493 + BWI76_RS16600 0.60 +0.2 3,438,493 + BWI76_RS16600 0.60 -0.3 3,438,494 + BWI76_RS16600 0.60 +0.4 3,438,501 - BWI76_RS16600 0.62 +0.0 3,438,536 + BWI76_RS16600 0.70 -1.1 3,438,571 + -0.3 3,438,571 + BWI76_RS16600 0.78 -2.1 3,438,571 + BWI76_RS16600 0.78 +0.1 3,438,571 + BWI76_RS16600 0.78 -0.3 3,438,571 + BWI76_RS16600 0.78 -2.5 3,438,578 + BWI76_RS16600 0.79 +0.3 3,438,579 - BWI76_RS16600 0.79 +2.4 3,438,579 - BWI76_RS16600 0.79 +0.2 3,438,579 - BWI76_RS16600 0.79 -0.8 3,438,579 - BWI76_RS16600 0.79 +0.4 3,438,579 - BWI76_RS16600 0.79 +0.2 3,438,579 - BWI76_RS16600 0.79 +0.2 3,438,579 - BWI76_RS16600 0.79 -0.4 3,438,579 - BWI76_RS16600 0.79 -1.3 3,438,579 - BWI76_RS16600 0.79 -3.5 3,438,579 - BWI76_RS16600 0.79 +0.4 3,438,579 - BWI76_RS16600 0.79 +0.8 3,438,579 - BWI76_RS16600 0.79 +0.4 3,438,593 - BWI76_RS16600 0.83 -0.3 3,438,668 - -1.8
Or see this region's nucleotide sequence