Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS13550

Experiment: RCH2_defined_Glucose_noNitrogen in EcoFAB_3.5mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS13540 and BWI76_RS13545 are separated by 11 nucleotidesBWI76_RS13545 and BWI76_RS13550 are separated by 9 nucleotidesBWI76_RS13550 and BWI76_RS13555 overlap by 17 nucleotidesBWI76_RS13555 and BWI76_RS13560 overlap by 4 nucleotides BWI76_RS13540: BWI76_RS13540 - ABC transporter substrate-binding protein, at 2,823,528 to 2,824,469 _RS13540 BWI76_RS13545: BWI76_RS13545 - N-acetyltransferase, at 2,824,481 to 2,824,990 _RS13545 BWI76_RS13550: BWI76_RS13550 - polar amino acid ABC transporter inner membrane subunit, at 2,825,000 to 2,825,926 _RS13550 BWI76_RS13555: BWI76_RS13555 - ABC transporter, at 2,825,910 to 2,826,689 _RS13555 BWI76_RS13560: BWI76_RS13560 - amino acid ABC transporter substrate-binding subunit, at 2,826,686 to 2,827,579 _RS13560 Position (kb) 2824 2825 2826Strain fitness (log2 ratio) -1 0 1 2 3 4at 2824.032 kb on + strand, within BWI76_RS13540at 2824.100 kb on - strand, within BWI76_RS13540at 2824.167 kb on + strand, within BWI76_RS13540at 2824.175 kb on - strand, within BWI76_RS13540at 2824.910 kb on + strand, within BWI76_RS13545at 2825.210 kb on + strand, within BWI76_RS13550at 2825.514 kb on - strand, within BWI76_RS13550at 2825.514 kb on - strand, within BWI76_RS13550at 2825.538 kb on - strand, within BWI76_RS13550at 2825.652 kb on - strand, within BWI76_RS13550at 2825.689 kb on + strand, within BWI76_RS13550at 2825.697 kb on - strand, within BWI76_RS13550at 2825.716 kb on - strand, within BWI76_RS13550at 2825.791 kb on + strand, within BWI76_RS13550at 2825.902 kb on - strandat 2825.906 kb on + strandat 2826.466 kb on - strand, within BWI76_RS13555

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_Glucose_noNitrogen in EcoFAB_3.5mL
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2,824,032 + BWI76_RS13540 0.54 +2.4
2,824,100 - BWI76_RS13540 0.61 -0.8
2,824,167 + BWI76_RS13540 0.68 +1.3
2,824,175 - BWI76_RS13540 0.69 +0.7
2,824,910 + BWI76_RS13545 0.84 -0.1
2,825,210 + BWI76_RS13550 0.23 +4.2
2,825,514 - BWI76_RS13550 0.55 -0.5
2,825,514 - BWI76_RS13550 0.55 +0.1
2,825,538 - BWI76_RS13550 0.58 +0.4
2,825,652 - BWI76_RS13550 0.70 -0.1
2,825,689 + BWI76_RS13550 0.74 +1.6
2,825,697 - BWI76_RS13550 0.75 +0.8
2,825,716 - BWI76_RS13550 0.77 +0.0
2,825,791 + BWI76_RS13550 0.85 +0.1
2,825,902 - +0.2
2,825,906 + -0.7
2,826,466 - BWI76_RS13555 0.71 -0.2

Or see this region's nucleotide sequence