Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS10435

Experiment: RCH2_defined_Glucose_noNitrogen in EcoFAB_3.5mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS10425 and BWI76_RS10430 are separated by 107 nucleotidesBWI76_RS10430 and BWI76_RS10435 are separated by 44 nucleotidesBWI76_RS10435 and BWI76_RS10440 are separated by 7 nucleotides BWI76_RS10425: BWI76_RS10425 - AIG2 family protein, at 2,204,434 to 2,204,769 _RS10425 BWI76_RS10430: BWI76_RS10430 - thiol:disulfide interchange protein DsbG, at 2,204,877 to 2,205,635 _RS10430 BWI76_RS10435: BWI76_RS10435 - suppressor for copper-sensitivity C, at 2,205,680 to 2,206,402 _RS10435 BWI76_RS10440: BWI76_RS10440 - protein-disulfide reductase, at 2,206,410 to 2,208,434 _RS10440 Position (kb) 2205 2206 2207Strain fitness (log2 ratio) -1 0 1at 2204.744 kb on - strandat 2205.247 kb on + strand, within BWI76_RS10430at 2205.360 kb on + strand, within BWI76_RS10430at 2205.445 kb on + strand, within BWI76_RS10430at 2205.491 kb on - strand, within BWI76_RS10430at 2205.589 kb on - strandat 2205.739 kb on - strandat 2205.802 kb on + strand, within BWI76_RS10435at 2206.100 kb on + strand, within BWI76_RS10435at 2206.108 kb on - strand, within BWI76_RS10435at 2206.505 kb on + strandat 2206.705 kb on + strand, within BWI76_RS10440at 2206.789 kb on + strand, within BWI76_RS10440at 2207.397 kb on - strand, within BWI76_RS10440

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_Glucose_noNitrogen in EcoFAB_3.5mL
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2,204,744 - +0.4
2,205,247 + BWI76_RS10430 0.49 +0.7
2,205,360 + BWI76_RS10430 0.64 +0.3
2,205,445 + BWI76_RS10430 0.75 +0.3
2,205,491 - BWI76_RS10430 0.81 +0.4
2,205,589 - +0.5
2,205,739 - -0.4
2,205,802 + BWI76_RS10435 0.17 -1.1
2,206,100 + BWI76_RS10435 0.58 -1.1
2,206,108 - BWI76_RS10435 0.59 -0.8
2,206,505 + -0.6
2,206,705 + BWI76_RS10440 0.15 -0.2
2,206,789 + BWI76_RS10440 0.19 +0.3
2,207,397 - BWI76_RS10440 0.49 -1.1

Or see this region's nucleotide sequence