Experiment: RCH2_defined_Glucose_noNitrogen in EcoFAB_3.5mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS06945 and BWI76_RS06950 overlap by 63 nucleotides BWI76_RS06950 and BWI76_RS06955 overlap by 4 nucleotides BWI76_RS06955 and BWI76_RS06960 are separated by 70 nucleotides BWI76_RS06960 and BWI76_RS06965 are separated by 209 nucleotides
BWI76_RS06945: BWI76_RS06945 - multifunctional acyl-CoA thioesterase I/protease I/lysophospholipase L1, at 1,458,356 to 1,459,012
_RS06945
BWI76_RS06950: BWI76_RS06950 - ABC transporter ATP-binding protein, at 1,458,950 to 1,459,636
_RS06950
BWI76_RS06955: BWI76_RS06955 - sugar ABC transporter permease, at 1,459,633 to 1,462,050
_RS06955
BWI76_RS06960: BWI76_RS06960 - monooxygenase, at 1,462,121 to 1,462,426
_RS06960
BWI76_RS06965: BWI76_RS06965 - membrane protein, at 1,462,636 to 1,463,781
_RS06965
Position (kb) 1459
1460
1461
1462
1463 Strain fitness (log2 ratio) -1
0
1
2
3
4
5
6
7 at 1458.679 kb on + strand, within BWI76_RS06945 at 1458.819 kb on + strand, within BWI76_RS06945 at 1458.827 kb on - strand, within BWI76_RS06945 at 1459.254 kb on + strand, within BWI76_RS06950 at 1459.345 kb on - strand, within BWI76_RS06950 at 1459.445 kb on + strand, within BWI76_RS06950 at 1459.816 kb on - strand at 1459.821 kb on - strand at 1459.849 kb on + strand at 1459.993 kb on - strand, within BWI76_RS06955 at 1460.075 kb on + strand, within BWI76_RS06955 at 1460.075 kb on + strand, within BWI76_RS06955 at 1460.123 kb on + strand, within BWI76_RS06955 at 1460.321 kb on + strand, within BWI76_RS06955 at 1460.849 kb on - strand, within BWI76_RS06955 at 1461.119 kb on + strand, within BWI76_RS06955 at 1461.143 kb on - strand, within BWI76_RS06955 at 1461.243 kb on - strand, within BWI76_RS06955 at 1461.256 kb on + strand, within BWI76_RS06955 at 1461.366 kb on - strand, within BWI76_RS06955 at 1461.869 kb on + strand at 1462.278 kb on + strand, within BWI76_RS06960 at 1462.692 kb on - strand at 1462.801 kb on + strand, within BWI76_RS06965 at 1462.891 kb on - strand, within BWI76_RS06965 at 1462.938 kb on - strand, within BWI76_RS06965 at 1462.991 kb on - strand, within BWI76_RS06965 at 1463.012 kb on - strand, within BWI76_RS06965 at 1463.012 kb on - strand, within BWI76_RS06965
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_Glucose_noNitrogen in EcoFAB_3.5mL remove 1,458,679 + BWI76_RS06945 0.49 -1.4 1,458,819 + BWI76_RS06945 0.70 -0.1 1,458,827 - BWI76_RS06945 0.72 +1.4 1,459,254 + BWI76_RS06950 0.44 +0.3 1,459,345 - BWI76_RS06950 0.57 -0.8 1,459,445 + BWI76_RS06950 0.72 -0.1 1,459,816 - -0.5 1,459,821 - -1.4 1,459,849 + +0.0 1,459,993 - BWI76_RS06955 0.15 -0.2 1,460,075 + BWI76_RS06955 0.18 +0.5 1,460,075 + BWI76_RS06955 0.18 +0.2 1,460,123 + BWI76_RS06955 0.20 +0.6 1,460,321 + BWI76_RS06955 0.28 -0.1 1,460,849 - BWI76_RS06955 0.50 +6.8 1,461,119 + BWI76_RS06955 0.61 +0.6 1,461,143 - BWI76_RS06955 0.62 +0.4 1,461,243 - BWI76_RS06955 0.67 -0.4 1,461,256 + BWI76_RS06955 0.67 +0.4 1,461,366 - BWI76_RS06955 0.72 -0.6 1,461,869 + +2.2 1,462,278 + BWI76_RS06960 0.51 -1.4 1,462,692 - +0.6 1,462,801 + BWI76_RS06965 0.14 +0.6 1,462,891 - BWI76_RS06965 0.22 -0.6 1,462,938 - BWI76_RS06965 0.26 +0.7 1,462,991 - BWI76_RS06965 0.31 -0.2 1,463,012 - BWI76_RS06965 0.33 -0.6 1,463,012 - BWI76_RS06965 0.33 -0.3
Or see this region's nucleotide sequence