Experiment: RCH2_defined_Glucose_noNitrogen in EcoFAB_3.5mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS03175 and BWI76_RS03180 are separated by 550 nucleotides BWI76_RS03180 and BWI76_RS03185 overlap by 4 nucleotides BWI76_RS03185 and BWI76_RS03190 overlap by 4 nucleotides BWI76_RS03190 and BWI76_RS03195 overlap by 4 nucleotides BWI76_RS03195 and BWI76_RS03200 are separated by 11 nucleotides
BWI76_RS03175: BWI76_RS03175 - hypothetical protein, at 655,058 to 655,795
_RS03175
BWI76_RS03180: BWI76_RS03180 - phage transcriptional regulator, AlpA, at 656,346 to 656,612
_RS03180
BWI76_RS03185: BWI76_RS03185 - CI repressor, at 656,609 to 657,166
_RS03185
BWI76_RS03190: BWI76_RS03190 - hypothetical protein, at 657,163 to 657,390
_RS03190
BWI76_RS03195: BWI76_RS03195 - P4 phage protein, at 657,387 to 657,707
_RS03195
BWI76_RS03200: BWI76_RS03200 - nucleoside triphosphatase, D5 family protein, at 657,719 to 660,052
_RS03200
Position (kb)
656
657
658 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 655.714 kb on + strand, within BWI76_RS03175 at 655.714 kb on + strand, within BWI76_RS03175 at 655.733 kb on + strand at 656.051 kb on + strand at 656.059 kb on - strand at 656.059 kb on - strand at 656.292 kb on + strand at 656.402 kb on + strand, within BWI76_RS03180 at 656.402 kb on + strand, within BWI76_RS03180 at 656.410 kb on - strand, within BWI76_RS03180 at 656.784 kb on - strand, within BWI76_RS03185 at 656.802 kb on + strand, within BWI76_RS03185 at 656.868 kb on - strand, within BWI76_RS03185 at 656.960 kb on - strand, within BWI76_RS03185 at 656.994 kb on - strand, within BWI76_RS03185 at 657.258 kb on + strand, within BWI76_RS03190 at 657.260 kb on + strand, within BWI76_RS03190 at 657.330 kb on + strand, within BWI76_RS03190 at 657.416 kb on - strand at 657.435 kb on + strand, within BWI76_RS03195 at 657.453 kb on - strand, within BWI76_RS03195 at 657.476 kb on - strand, within BWI76_RS03195 at 657.884 kb on - strand at 657.931 kb on + strand at 658.035 kb on - strand, within BWI76_RS03200 at 658.107 kb on + strand, within BWI76_RS03200
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_Glucose_noNitrogen in EcoFAB_3.5mL remove 655,714 + BWI76_RS03175 0.89 -1.0 655,714 + BWI76_RS03175 0.89 +0.0 655,733 + -0.3 656,051 + -1.9 656,059 - -0.5 656,059 - +3.2 656,292 + +0.0 656,402 + BWI76_RS03180 0.21 -2.1 656,402 + BWI76_RS03180 0.21 -0.7 656,410 - BWI76_RS03180 0.24 +3.7 656,784 - BWI76_RS03185 0.31 +1.9 656,802 + BWI76_RS03185 0.35 +1.1 656,868 - BWI76_RS03185 0.46 +2.4 656,960 - BWI76_RS03185 0.63 +0.5 656,994 - BWI76_RS03185 0.69 -0.1 657,258 + BWI76_RS03190 0.42 -0.2 657,260 + BWI76_RS03190 0.43 +2.0 657,330 + BWI76_RS03190 0.73 +0.5 657,416 - -0.7 657,435 + BWI76_RS03195 0.15 +0.2 657,453 - BWI76_RS03195 0.21 +0.3 657,476 - BWI76_RS03195 0.28 +0.6 657,884 - -0.8 657,931 + +0.1 658,035 - BWI76_RS03200 0.14 -0.2 658,107 + BWI76_RS03200 0.17 -1.1
Or see this region's nucleotide sequence