Experiment: RCH2_defined_Glucose_noNitrogen in EcoFAB_3.5mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01770 and BWI76_RS01775 are separated by 105 nucleotides BWI76_RS01775 and BWI76_RS01780 are separated by 138 nucleotides BWI76_RS01780 and BWI76_RS01785 are separated by 5 nucleotides BWI76_RS01785 and BWI76_RS01790 are separated by 38 nucleotides
BWI76_RS01770: BWI76_RS01770 - transcriptional regulatory protein RtcR, at 361,143 to 362,723
_RS01770
BWI76_RS01775: BWI76_RS01775 - hypothetical protein, at 362,829 to 363,032
_RS01775
BWI76_RS01780: BWI76_RS01780 - SPFH domain / Band 7 family protein, at 363,171 to 364,298
_RS01780
BWI76_RS01785: BWI76_RS01785 - nucleotidyltransferase, at 364,304 to 365,092
_RS01785
BWI76_RS01790: BWI76_RS01790 - lysine-sensitive aspartokinase 3, at 365,131 to 366,480
_RS01790
Position (kb) 363
364
365 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 362.224 kb on - strand, within BWI76_RS01770 at 362.307 kb on - strand, within BWI76_RS01770 at 362.307 kb on - strand, within BWI76_RS01770 at 362.374 kb on - strand, within BWI76_RS01770 at 362.439 kb on + strand at 362.460 kb on - strand, within BWI76_RS01770 at 362.460 kb on - strand, within BWI76_RS01770 at 362.460 kb on - strand, within BWI76_RS01770 at 362.584 kb on + strand at 362.632 kb on - strand at 362.689 kb on - strand at 362.916 kb on - strand, within BWI76_RS01775 at 363.055 kb on - strand at 363.055 kb on - strand at 363.055 kb on - strand at 363.066 kb on + strand at 363.066 kb on + strand at 363.066 kb on + strand at 363.066 kb on + strand at 363.066 kb on + strand at 363.074 kb on - strand at 363.074 kb on - strand at 363.074 kb on - strand at 363.074 kb on - strand at 363.240 kb on - strand at 363.272 kb on + strand at 363.374 kb on + strand, within BWI76_RS01780 at 363.377 kb on - strand, within BWI76_RS01780 at 363.419 kb on - strand, within BWI76_RS01780 at 363.521 kb on + strand, within BWI76_RS01780 at 363.617 kb on + strand, within BWI76_RS01780 at 363.635 kb on + strand, within BWI76_RS01780 at 363.974 kb on + strand, within BWI76_RS01780 at 364.030 kb on + strand, within BWI76_RS01780 at 364.049 kb on + strand, within BWI76_RS01780 at 364.122 kb on - strand, within BWI76_RS01780 at 364.145 kb on + strand, within BWI76_RS01780 at 364.211 kb on + strand at 364.216 kb on + strand at 364.216 kb on + strand at 364.454 kb on - strand, within BWI76_RS01785 at 364.515 kb on + strand, within BWI76_RS01785 at 364.854 kb on + strand, within BWI76_RS01785 at 364.923 kb on - strand, within BWI76_RS01785 at 364.928 kb on - strand, within BWI76_RS01785 at 364.986 kb on + strand, within BWI76_RS01785 at 364.996 kb on + strand, within BWI76_RS01785 at 365.004 kb on - strand, within BWI76_RS01785 at 365.065 kb on + strand at 365.273 kb on - strand, within BWI76_RS01790
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_Glucose_noNitrogen in EcoFAB_3.5mL remove 362,224 - BWI76_RS01770 0.68 +0.6 362,307 - BWI76_RS01770 0.74 -0.1 362,307 - BWI76_RS01770 0.74 +2.3 362,374 - BWI76_RS01770 0.78 -0.8 362,439 + +1.7 362,460 - BWI76_RS01770 0.83 -1.8 362,460 - BWI76_RS01770 0.83 +0.4 362,460 - BWI76_RS01770 0.83 -0.3 362,584 + +0.3 362,632 - +0.9 362,689 - +0.3 362,916 - BWI76_RS01775 0.43 +0.7 363,055 - +1.1 363,055 - -1.1 363,055 - -0.3 363,066 + -0.4 363,066 + +1.7 363,066 + +0.2 363,066 + -0.2 363,066 + +0.1 363,074 - -0.8 363,074 - +1.7 363,074 - +0.1 363,074 - +0.3 363,240 - -0.1 363,272 + -0.5 363,374 + BWI76_RS01780 0.18 +1.9 363,377 - BWI76_RS01780 0.18 +2.4 363,419 - BWI76_RS01780 0.22 +3.6 363,521 + BWI76_RS01780 0.31 -0.5 363,617 + BWI76_RS01780 0.40 +0.0 363,635 + BWI76_RS01780 0.41 +0.4 363,974 + BWI76_RS01780 0.71 +0.7 364,030 + BWI76_RS01780 0.76 -0.3 364,049 + BWI76_RS01780 0.78 +1.8 364,122 - BWI76_RS01780 0.84 +0.1 364,145 + BWI76_RS01780 0.86 +0.5 364,211 + +0.2 364,216 + +1.7 364,216 + -1.3 364,454 - BWI76_RS01785 0.19 +0.2 364,515 + BWI76_RS01785 0.27 -0.3 364,854 + BWI76_RS01785 0.70 +4.2 364,923 - BWI76_RS01785 0.78 -0.0 364,928 - BWI76_RS01785 0.79 -0.2 364,986 + BWI76_RS01785 0.86 -0.1 364,996 + BWI76_RS01785 0.88 -1.9 365,004 - BWI76_RS01785 0.89 -0.5 365,065 + +0.5 365,273 - BWI76_RS01790 0.11 +0.7
Or see this region's nucleotide sequence