Experiment: RCH2_defined_Glucose_noNitrogen in EcoFAB_3.5mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00970 and BWI76_RS00975 are separated by 60 nucleotides BWI76_RS00975 and BWI76_RS00980 are separated by 2 nucleotides
BWI76_RS00970: BWI76_RS00970 - branched chain amino acid aminotransferase, at 197,304 to 198,233
_RS00970
BWI76_RS00975: BWI76_RS00975 - dihydroxy-acid dehydratase, at 198,294 to 200,144
_RS00975
BWI76_RS00980: BWI76_RS00980 - PLP-dependent threonine dehydratase, at 200,147 to 201,691
_RS00980
Position (kb) 198
199
200
201 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 197.330 kb on - strand at 197.383 kb on + strand at 197.399 kb on - strand, within BWI76_RS00970 at 197.484 kb on + strand, within BWI76_RS00970 at 197.484 kb on + strand, within BWI76_RS00970 at 197.540 kb on + strand, within BWI76_RS00970 at 197.540 kb on + strand, within BWI76_RS00970 at 197.686 kb on + strand, within BWI76_RS00970 at 198.044 kb on + strand, within BWI76_RS00970 at 198.112 kb on + strand, within BWI76_RS00970 at 198.168 kb on + strand at 198.297 kb on + strand at 198.442 kb on + strand at 198.442 kb on + strand at 198.543 kb on + strand, within BWI76_RS00975 at 198.551 kb on - strand, within BWI76_RS00975 at 198.608 kb on + strand, within BWI76_RS00975 at 198.636 kb on - strand, within BWI76_RS00975 at 198.648 kb on + strand, within BWI76_RS00975 at 198.849 kb on + strand, within BWI76_RS00975 at 198.850 kb on - strand, within BWI76_RS00975 at 198.850 kb on - strand, within BWI76_RS00975 at 199.017 kb on - strand, within BWI76_RS00975 at 199.040 kb on + strand, within BWI76_RS00975 at 199.043 kb on - strand, within BWI76_RS00975 at 199.226 kb on + strand, within BWI76_RS00975 at 199.226 kb on + strand, within BWI76_RS00975 at 199.226 kb on + strand, within BWI76_RS00975 at 199.226 kb on + strand, within BWI76_RS00975 at 199.234 kb on - strand, within BWI76_RS00975 at 199.234 kb on - strand, within BWI76_RS00975 at 199.740 kb on - strand, within BWI76_RS00975 at 199.806 kb on + strand, within BWI76_RS00975 at 199.877 kb on - strand, within BWI76_RS00975 at 199.960 kb on - strand at 200.009 kb on + strand at 200.062 kb on + strand at 200.070 kb on - strand at 200.192 kb on - strand at 200.207 kb on - strand at 200.207 kb on - strand at 200.229 kb on - strand at 200.244 kb on - strand at 200.325 kb on + strand, within BWI76_RS00980 at 200.325 kb on + strand, within BWI76_RS00980 at 200.325 kb on + strand, within BWI76_RS00980 at 200.333 kb on - strand, within BWI76_RS00980 at 200.333 kb on - strand, within BWI76_RS00980 at 200.333 kb on - strand, within BWI76_RS00980 at 200.333 kb on - strand, within BWI76_RS00980 at 200.333 kb on - strand, within BWI76_RS00980 at 200.333 kb on - strand, within BWI76_RS00980 at 200.333 kb on - strand, within BWI76_RS00980 at 200.333 kb on - strand, within BWI76_RS00980 at 200.333 kb on - strand, within BWI76_RS00980 at 200.333 kb on - strand, within BWI76_RS00980 at 200.558 kb on + strand, within BWI76_RS00980 at 200.652 kb on - strand, within BWI76_RS00980 at 200.792 kb on + strand, within BWI76_RS00980 at 200.845 kb on - strand, within BWI76_RS00980 at 200.845 kb on - strand, within BWI76_RS00980 at 200.869 kb on - strand, within BWI76_RS00980 at 200.929 kb on + strand, within BWI76_RS00980
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_Glucose_noNitrogen in EcoFAB_3.5mL remove 197,330 - -1.6 197,383 + -2.1 197,399 - BWI76_RS00970 0.10 +0.0 197,484 + BWI76_RS00970 0.19 +0.5 197,484 + BWI76_RS00970 0.19 +0.1 197,540 + BWI76_RS00970 0.25 -1.1 197,540 + BWI76_RS00970 0.25 +1.9 197,686 + BWI76_RS00970 0.41 -1.1 198,044 + BWI76_RS00970 0.80 +0.4 198,112 + BWI76_RS00970 0.87 -0.1 198,168 + +0.4 198,297 + -0.4 198,442 + +0.0 198,442 + -2.0 198,543 + BWI76_RS00975 0.13 -0.7 198,551 - BWI76_RS00975 0.14 -0.5 198,608 + BWI76_RS00975 0.17 +0.4 198,636 - BWI76_RS00975 0.18 -2.0 198,648 + BWI76_RS00975 0.19 -0.9 198,849 + BWI76_RS00975 0.30 +0.7 198,850 - BWI76_RS00975 0.30 -0.9 198,850 - BWI76_RS00975 0.30 -1.4 199,017 - BWI76_RS00975 0.39 -1.2 199,040 + BWI76_RS00975 0.40 -0.9 199,043 - BWI76_RS00975 0.40 -0.5 199,226 + BWI76_RS00975 0.50 -1.1 199,226 + BWI76_RS00975 0.50 -0.1 199,226 + BWI76_RS00975 0.50 -1.7 199,226 + BWI76_RS00975 0.50 +1.8 199,234 - BWI76_RS00975 0.51 -1.9 199,234 - BWI76_RS00975 0.51 +0.1 199,740 - BWI76_RS00975 0.78 -0.9 199,806 + BWI76_RS00975 0.82 -1.1 199,877 - BWI76_RS00975 0.86 -0.9 199,960 - -2.9 200,009 + +0.2 200,062 + -0.3 200,070 - -0.5 200,192 - -1.1 200,207 - +0.2 200,207 - -0.2 200,229 - +0.8 200,244 - -1.2 200,325 + BWI76_RS00980 0.12 -0.7 200,325 + BWI76_RS00980 0.12 -0.9 200,325 + BWI76_RS00980 0.12 -1.0 200,333 - BWI76_RS00980 0.12 -1.8 200,333 - BWI76_RS00980 0.12 -2.5 200,333 - BWI76_RS00980 0.12 +0.1 200,333 - BWI76_RS00980 0.12 -1.0 200,333 - BWI76_RS00980 0.12 -1.6 200,333 - BWI76_RS00980 0.12 -2.5 200,333 - BWI76_RS00980 0.12 +0.0 200,333 - BWI76_RS00980 0.12 -0.6 200,333 - BWI76_RS00980 0.12 -0.2 200,333 - BWI76_RS00980 0.12 -0.6 200,558 + BWI76_RS00980 0.27 -1.6 200,652 - BWI76_RS00980 0.33 +2.0 200,792 + BWI76_RS00980 0.42 +1.2 200,845 - BWI76_RS00980 0.45 +0.1 200,845 - BWI76_RS00980 0.45 -0.3 200,869 - BWI76_RS00980 0.47 -1.9 200,929 + BWI76_RS00980 0.51 -0.9
Or see this region's nucleotide sequence