Experiment: MoLS4 with L-Glutamine as nutrient 10mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU0275 and tRNA-Val are separated by 495 nucleotides tRNA-Val and tRNA-Asp are separated by 3 nucleotides tRNA-Asp and tRNA-Asp are separated by 71 nucleotides tRNA-Asp and DVU0276 are separated by 265 nucleotides DVU0276 and DORF26828 are separated by 147 nucleotides
DVU0275: DVU0275 - polysaccharide deacetylase family protein (TIGR), at 317,644 to 318,339
DVU0275
DVU_tRNA-Val-1: tRNA-Val - tRNA with anticodon CAC for Val (VIMSS-AUTO), at 318,835 to 318,910
tRNA-Val
DVU_tRNA-Asp-1: tRNA-Asp - tRNA with anticodon GTC for Asp (VIMSS-AUTO), at 318,914 to 318,990
tRNA-Asp
DVU_tRNA-Asp-2: tRNA-Asp - tRNA with anticodon GTC for Asp (VIMSS-AUTO), at 319,062 to 319,138
tRNA-Asp
DVU0276: DVU0276 - conserved hypothetical protein (TIGR), at 319,404 to 319,802
DVU0276
DORF26828: DORF26828 - SEED: FIG00605560: hypothetical protein, at 319,950 to 320,180
DORF26828
Position (kb) 318
319 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 317.954 kb on + strand, within DVU0275 at 317.964 kb on + strand, within DVU0275 at 317.972 kb on - strand, within DVU0275 at 318.106 kb on + strand, within DVU0275 at 318.145 kb on - strand, within DVU0275 at 318.169 kb on + strand, within DVU0275 at 318.181 kb on + strand, within DVU0275 at 318.187 kb on - strand, within DVU0275 at 318.189 kb on - strand, within DVU0275 at 318.212 kb on + strand, within DVU0275 at 318.218 kb on + strand, within DVU0275 at 318.287 kb on + strand at 318.292 kb on + strand at 318.324 kb on - strand at 318.342 kb on - strand at 318.424 kb on + strand at 318.462 kb on - strand at 318.585 kb on + strand at 318.585 kb on + strand at 318.593 kb on - strand at 318.667 kb on + strand at 318.667 kb on + strand at 318.677 kb on + strand at 318.685 kb on - strand at 318.692 kb on + strand at 318.779 kb on + strand at 319.161 kb on + strand at 319.265 kb on + strand at 319.364 kb on + strand at 319.394 kb on - strand at 319.401 kb on + strand at 319.411 kb on + strand at 319.411 kb on + strand at 319.419 kb on - strand at 319.458 kb on - strand, within DVU0276 at 319.474 kb on - strand, within DVU0276 at 319.476 kb on + strand, within DVU0276 at 319.553 kb on + strand, within DVU0276 at 319.563 kb on + strand, within DVU0276 at 319.568 kb on + strand, within DVU0276 at 319.583 kb on - strand, within DVU0276 at 319.587 kb on + strand, within DVU0276 at 319.595 kb on - strand, within DVU0276 at 319.605 kb on - strand, within DVU0276 at 319.619 kb on + strand, within DVU0276 at 319.640 kb on - strand, within DVU0276 at 319.658 kb on + strand, within DVU0276 at 319.658 kb on + strand, within DVU0276 at 319.697 kb on - strand, within DVU0276 at 319.711 kb on + strand, within DVU0276 at 319.715 kb on + strand, within DVU0276 at 319.723 kb on - strand, within DVU0276 at 319.723 kb on - strand, within DVU0276 at 319.734 kb on + strand, within DVU0276 at 319.734 kb on + strand, within DVU0276 at 319.743 kb on - strand, within DVU0276 at 319.779 kb on + strand at 319.787 kb on - strand at 319.787 kb on - strand at 319.789 kb on - strand at 319.843 kb on + strand at 319.843 kb on + strand at 319.848 kb on + strand at 319.856 kb on - strand at 319.856 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Glutamine as nutrient 10mM remove 317,954 + DVU0275 0.45 -3.2 317,964 + DVU0275 0.46 -2.6 317,972 - DVU0275 0.47 +1.2 318,106 + DVU0275 0.66 +0.7 318,145 - DVU0275 0.72 +0.3 318,169 + DVU0275 0.75 -0.1 318,181 + DVU0275 0.77 -1.4 318,187 - DVU0275 0.78 -0.0 318,189 - DVU0275 0.78 +0.1 318,212 + DVU0275 0.82 -0.8 318,218 + DVU0275 0.82 -1.6 318,287 + -2.0 318,292 + -3.6 318,324 - -0.3 318,342 - -0.6 318,424 + -0.4 318,462 - -3.4 318,585 + -0.1 318,585 + -2.6 318,593 - -2.7 318,667 + +0.3 318,667 + -0.2 318,677 + -0.3 318,685 - -2.7 318,692 + -0.2 318,779 + +0.3 319,161 + -0.1 319,265 + -3.3 319,364 + -3.2 319,394 - -0.8 319,401 + -1.2 319,411 + -3.7 319,411 + -0.4 319,419 - +0.0 319,458 - DVU0276 0.14 -2.5 319,474 - DVU0276 0.18 -0.0 319,476 + DVU0276 0.18 +1.2 319,553 + DVU0276 0.37 -0.6 319,563 + DVU0276 0.40 +0.0 319,568 + DVU0276 0.41 -1.0 319,583 - DVU0276 0.45 -0.1 319,587 + DVU0276 0.46 +0.4 319,595 - DVU0276 0.48 -0.1 319,605 - DVU0276 0.50 +0.1 319,619 + DVU0276 0.54 +0.5 319,640 - DVU0276 0.59 -4.8 319,658 + DVU0276 0.64 -0.6 319,658 + DVU0276 0.64 -0.1 319,697 - DVU0276 0.73 -0.4 319,711 + DVU0276 0.77 -0.7 319,715 + DVU0276 0.78 +0.4 319,723 - DVU0276 0.80 -1.2 319,723 - DVU0276 0.80 -0.3 319,734 + DVU0276 0.83 -0.1 319,734 + DVU0276 0.83 +0.4 319,743 - DVU0276 0.85 +0.4 319,779 + +0.0 319,787 - -2.1 319,787 - -0.1 319,789 - +0.4 319,843 + -0.6 319,843 + -0.2 319,848 + +0.3 319,856 - +0.1 319,856 - +0.1
Or see this region's nucleotide sequence