Experiment: MoLS4 with L-Glutamine as nutrient 10mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt topB and DVU2317 are separated by 289 nucleotides DVU2317 and rbr2 are separated by 611 nucleotides
DVU2316: topB - DNA topoisomerase III (TIGR), at 2,408,456 to 2,411,044
topB
DVU2317: DVU2317 - methyl-accepting chemotaxis protein, putative (TIGR), at 2,411,334 to 2,413,103
DVU2317
DVU2318: rbr2 - rubrerythrin, putative (TIGR), at 2,413,715 to 2,414,209
rbr2
Position (kb)
2411
2412
2413
2414 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2410.456 kb on + strand, within topB at 2410.474 kb on + strand, within topB at 2410.608 kb on - strand, within topB at 2410.689 kb on - strand, within topB at 2410.738 kb on + strand, within topB at 2410.745 kb on + strand, within topB at 2410.777 kb on + strand, within topB at 2410.785 kb on - strand, within topB at 2410.887 kb on - strand at 2411.086 kb on - strand at 2411.102 kb on + strand at 2411.103 kb on - strand at 2411.180 kb on - strand at 2411.289 kb on + strand at 2411.442 kb on - strand at 2411.458 kb on + strand at 2411.564 kb on + strand, within DVU2317 at 2411.616 kb on + strand, within DVU2317 at 2412.015 kb on - strand, within DVU2317 at 2412.098 kb on + strand, within DVU2317 at 2412.103 kb on + strand, within DVU2317 at 2412.128 kb on - strand, within DVU2317 at 2412.135 kb on - strand, within DVU2317 at 2412.160 kb on - strand, within DVU2317 at 2412.197 kb on - strand, within DVU2317 at 2412.277 kb on - strand, within DVU2317 at 2412.280 kb on + strand, within DVU2317 at 2412.293 kb on - strand, within DVU2317 at 2412.301 kb on + strand, within DVU2317 at 2412.329 kb on + strand, within DVU2317 at 2412.383 kb on - strand, within DVU2317 at 2412.405 kb on + strand, within DVU2317 at 2412.427 kb on + strand, within DVU2317 at 2412.517 kb on - strand, within DVU2317 at 2412.548 kb on + strand, within DVU2317 at 2412.585 kb on + strand, within DVU2317 at 2412.626 kb on - strand, within DVU2317 at 2412.916 kb on - strand, within DVU2317 at 2412.916 kb on - strand, within DVU2317 at 2412.939 kb on - strand at 2412.978 kb on - strand at 2413.020 kb on - strand at 2413.031 kb on + strand at 2413.088 kb on - strand at 2413.176 kb on - strand at 2413.192 kb on + strand at 2413.203 kb on + strand at 2413.203 kb on + strand at 2413.211 kb on - strand at 2413.211 kb on - strand at 2413.211 kb on - strand at 2413.244 kb on - strand at 2413.325 kb on + strand at 2413.350 kb on - strand at 2413.415 kb on + strand at 2413.538 kb on + strand at 2413.553 kb on + strand at 2413.597 kb on - strand at 2413.611 kb on - strand at 2413.759 kb on + strand at 2413.767 kb on - strand, within rbr2 at 2413.767 kb on - strand, within rbr2 at 2413.837 kb on - strand, within rbr2 at 2414.037 kb on - strand, within rbr2 at 2414.037 kb on - strand, within rbr2 at 2414.037 kb on - strand, within rbr2
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Glutamine as nutrient 10mM remove 2,410,456 + topB DVU2316 0.77 -0.3 2,410,474 + topB DVU2316 0.78 -3.2 2,410,608 - topB DVU2316 0.83 -0.5 2,410,689 - topB DVU2316 0.86 +0.1 2,410,738 + topB DVU2316 0.88 -0.5 2,410,745 + topB DVU2316 0.88 -0.9 2,410,777 + topB DVU2316 0.90 -0.2 2,410,785 - topB DVU2316 0.90 -1.1 2,410,887 - -0.1 2,411,086 - -0.9 2,411,102 + -0.0 2,411,103 - -2.6 2,411,180 - +0.7 2,411,289 + +0.1 2,411,442 - +0.4 2,411,458 + -0.1 2,411,564 + DVU2317 0.13 -0.2 2,411,616 + DVU2317 0.16 +0.0 2,412,015 - DVU2317 0.38 -0.3 2,412,098 + DVU2317 0.43 -3.1 2,412,103 + DVU2317 0.43 +0.1 2,412,128 - DVU2317 0.45 +1.2 2,412,135 - DVU2317 0.45 +0.2 2,412,160 - DVU2317 0.47 +0.4 2,412,197 - DVU2317 0.49 +0.3 2,412,277 - DVU2317 0.53 -1.8 2,412,280 + DVU2317 0.53 -0.3 2,412,293 - DVU2317 0.54 +0.0 2,412,301 + DVU2317 0.55 +0.5 2,412,329 + DVU2317 0.56 +0.0 2,412,383 - DVU2317 0.59 +1.1 2,412,405 + DVU2317 0.61 +2.2 2,412,427 + DVU2317 0.62 -0.8 2,412,517 - DVU2317 0.67 +0.2 2,412,548 + DVU2317 0.69 -0.3 2,412,585 + DVU2317 0.71 -0.2 2,412,626 - DVU2317 0.73 -0.1 2,412,916 - DVU2317 0.89 +0.8 2,412,916 - DVU2317 0.89 +0.3 2,412,939 - -0.2 2,412,978 - +0.5 2,413,020 - -0.6 2,413,031 + +0.1 2,413,088 - +0.8 2,413,176 - -0.5 2,413,192 + -0.2 2,413,203 + -2.2 2,413,203 + -0.0 2,413,211 - +0.3 2,413,211 - -0.4 2,413,211 - -0.5 2,413,244 - -0.1 2,413,325 + -0.8 2,413,350 - -2.7 2,413,415 + -0.1 2,413,538 + +0.1 2,413,553 + -0.5 2,413,597 - +0.5 2,413,611 - +0.2 2,413,759 + -2.3 2,413,767 - rbr2 DVU2318 0.11 -3.3 2,413,767 - rbr2 DVU2318 0.11 -0.0 2,413,837 - rbr2 DVU2318 0.25 +0.1 2,414,037 - rbr2 DVU2318 0.65 +0.3 2,414,037 - rbr2 DVU2318 0.65 -3.1 2,414,037 - rbr2 DVU2318 0.65 +0.0
Or see this region's nucleotide sequence