Experiment: MoLS4 with L-Glutamine as nutrient 10mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pyrF and DVU0902 are separated by 132 nucleotides DVU0902 and DVU0903 are separated by 4 nucleotides DVU0903 and recJ are separated by 56 nucleotides
DVU0901: pyrF - orotidine 5`-phosphate decarboxylase (TIGR), at 993,049 to 993,756
pyrF
DVU0902: DVU0902 - TPR domain protein (TIGR), at 993,889 to 994,746
DVU0902
DVU0903: DVU0903 - HD domain protein (TIGR), at 994,751 to 995,608
DVU0903
DVU0904: recJ - single-stranded-DNA-specific exonuclease RecJ (TIGR), at 995,665 to 997,380
recJ
Position (kb) 994
995
996 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 993.803 kb on + strand at 993.804 kb on + strand at 993.895 kb on - strand at 994.007 kb on - strand, within DVU0902 at 994.047 kb on - strand, within DVU0902 at 994.074 kb on - strand, within DVU0902 at 994.247 kb on - strand, within DVU0902 at 994.435 kb on - strand, within DVU0902 at 994.435 kb on - strand, within DVU0902 at 994.502 kb on - strand, within DVU0902 at 994.518 kb on + strand, within DVU0902 at 994.518 kb on + strand, within DVU0902 at 994.518 kb on - strand, within DVU0902 at 994.526 kb on - strand, within DVU0902 at 994.635 kb on + strand, within DVU0902 at 994.663 kb on + strand at 994.709 kb on - strand at 994.711 kb on + strand at 994.720 kb on + strand at 994.725 kb on - strand at 994.743 kb on - strand at 994.840 kb on - strand, within DVU0903 at 994.840 kb on - strand, within DVU0903 at 995.099 kb on + strand, within DVU0903 at 995.112 kb on - strand, within DVU0903 at 995.167 kb on + strand, within DVU0903 at 995.167 kb on + strand, within DVU0903 at 995.175 kb on - strand, within DVU0903 at 995.175 kb on - strand, within DVU0903 at 995.223 kb on + strand, within DVU0903 at 995.223 kb on + strand, within DVU0903 at 995.231 kb on - strand, within DVU0903 at 995.269 kb on + strand, within DVU0903 at 995.283 kb on + strand, within DVU0903 at 995.325 kb on + strand, within DVU0903 at 995.338 kb on - strand, within DVU0903 at 995.400 kb on + strand, within DVU0903 at 995.541 kb on - strand at 995.548 kb on + strand at 995.593 kb on - strand at 995.627 kb on - strand at 995.634 kb on - strand at 995.683 kb on - strand at 995.961 kb on - strand, within recJ at 996.290 kb on - strand, within recJ at 996.334 kb on + strand, within recJ at 996.342 kb on - strand, within recJ at 996.385 kb on + strand, within recJ at 996.393 kb on - strand, within recJ at 996.513 kb on + strand, within recJ
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Glutamine as nutrient 10mM remove 993,803 + +0.4 993,804 + -2.7 993,895 - -0.5 994,007 - DVU0902 0.14 -0.8 994,047 - DVU0902 0.18 -0.6 994,074 - DVU0902 0.22 -1.2 994,247 - DVU0902 0.42 +0.1 994,435 - DVU0902 0.64 +0.2 994,435 - DVU0902 0.64 -3.4 994,502 - DVU0902 0.71 +0.4 994,518 + DVU0902 0.73 +0.2 994,518 + DVU0902 0.73 -2.8 994,518 - DVU0902 0.73 -0.7 994,526 - DVU0902 0.74 +0.0 994,635 + DVU0902 0.87 -0.2 994,663 + -3.2 994,709 - -0.6 994,711 + +0.3 994,720 + -2.6 994,725 - -0.6 994,743 - -0.4 994,840 - DVU0903 0.10 -0.1 994,840 - DVU0903 0.10 +0.3 995,099 + DVU0903 0.41 -0.6 995,112 - DVU0903 0.42 -2.2 995,167 + DVU0903 0.48 -0.2 995,167 + DVU0903 0.48 -0.4 995,175 - DVU0903 0.49 +0.1 995,175 - DVU0903 0.49 -0.1 995,223 + DVU0903 0.55 -2.2 995,223 + DVU0903 0.55 +0.4 995,231 - DVU0903 0.56 -3.9 995,269 + DVU0903 0.60 -2.7 995,283 + DVU0903 0.62 -2.8 995,325 + DVU0903 0.67 -0.6 995,338 - DVU0903 0.68 +0.1 995,400 + DVU0903 0.76 +0.1 995,541 - +1.1 995,548 + -1.6 995,593 - -1.9 995,627 - +0.2 995,634 - -1.5 995,683 - -0.2 995,961 - recJ DVU0904 0.17 -2.2 996,290 - recJ DVU0904 0.36 +1.4 996,334 + recJ DVU0904 0.39 +0.5 996,342 - recJ DVU0904 0.39 +0.5 996,385 + recJ DVU0904 0.42 +1.1 996,393 - recJ DVU0904 0.42 -1.1 996,513 + recJ DVU0904 0.49 -2.0
Or see this region's nucleotide sequence