Experiment: MoLS4 with L-Glutamine as nutrient 10mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU0025 and tRNA-Ala are separated by 173 nucleotides tRNA-Ala and DVU0026 are separated by 364 nucleotides DVU0026 and DVU0027 are separated by 218 nucleotides
DVU0025: DVU0025 - sensory box histidine kinase (TIGR), at 27,714 to 29,873
DVU0025
DVU_tRNA-Ala-2: tRNA-Ala - tRNA with anticodon GGC for Ala (VIMSS-AUTO), at 30,047 to 30,122
tRNA-Ala
DVU0026: DVU0026 - conserved hypothetical protein (TIGR), at 30,487 to 31,059
DVU0026
DVU0027: DVU0027 - membrane protein, putative (TIGR), at 31,278 to 32,702
DVU0027
Position (kb)
30
31
32 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 29.497 kb on + strand, within DVU0025 at 29.546 kb on + strand, within DVU0025 at 29.569 kb on - strand, within DVU0025 at 29.571 kb on + strand, within DVU0025 at 29.579 kb on + strand, within DVU0025 at 29.596 kb on + strand, within DVU0025 at 29.676 kb on + strand at 29.702 kb on + strand at 29.722 kb on - strand at 29.758 kb on - strand at 29.778 kb on - strand at 29.789 kb on + strand at 29.866 kb on - strand at 29.906 kb on - strand at 29.933 kb on - strand at 29.940 kb on + strand at 29.952 kb on - strand at 30.091 kb on + strand, within tRNA-Ala at 30.227 kb on + strand at 30.240 kb on - strand at 30.305 kb on + strand at 30.366 kb on - strand at 30.379 kb on + strand at 30.485 kb on - strand at 30.708 kb on + strand, within DVU0026 at 30.763 kb on - strand, within DVU0026 at 30.809 kb on - strand, within DVU0026 at 30.818 kb on + strand, within DVU0026 at 30.833 kb on + strand, within DVU0026 at 30.849 kb on - strand, within DVU0026 at 30.909 kb on - strand, within DVU0026 at 30.938 kb on + strand, within DVU0026 at 30.938 kb on + strand, within DVU0026 at 31.008 kb on + strand at 31.052 kb on - strand at 31.080 kb on + strand at 31.252 kb on + strand at 31.364 kb on - strand at 31.371 kb on + strand at 31.374 kb on - strand at 31.437 kb on + strand, within DVU0027 at 31.532 kb on - strand, within DVU0027 at 31.591 kb on - strand, within DVU0027 at 31.623 kb on + strand, within DVU0027 at 31.660 kb on - strand, within DVU0027 at 31.832 kb on - strand, within DVU0027 at 31.832 kb on - strand, within DVU0027 at 31.993 kb on + strand, within DVU0027 at 31.993 kb on + strand, within DVU0027 at 31.998 kb on + strand, within DVU0027 at 32.044 kb on - strand, within DVU0027
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Glutamine as nutrient 10mM remove 29,497 + DVU0025 0.83 +0.1 29,546 + DVU0025 0.85 +0.1 29,569 - DVU0025 0.86 -0.5 29,571 + DVU0025 0.86 -0.4 29,579 + DVU0025 0.86 -0.4 29,596 + DVU0025 0.87 -0.2 29,676 + +0.3 29,702 + -2.2 29,722 - +0.8 29,758 - -0.4 29,778 - -0.1 29,789 + +2.0 29,866 - -0.1 29,906 - -2.3 29,933 - -0.5 29,940 + -1.3 29,952 - -0.0 30,091 + tRNA-Ala DVU_tRNA-Ala-2 0.58 +0.4 30,227 + -0.5 30,240 - -2.5 30,305 + -0.2 30,366 - +0.1 30,379 + -0.1 30,485 - +1.2 30,708 + DVU0026 0.39 -0.2 30,763 - DVU0026 0.48 -0.4 30,809 - DVU0026 0.56 -0.5 30,818 + DVU0026 0.58 -3.7 30,833 + DVU0026 0.60 -0.2 30,849 - DVU0026 0.63 +0.1 30,909 - DVU0026 0.74 +0.3 30,938 + DVU0026 0.79 +0.2 30,938 + DVU0026 0.79 -0.0 31,008 + -3.1 31,052 - +0.0 31,080 + -1.3 31,252 + -1.2 31,364 - -0.5 31,371 + -0.1 31,374 - -0.2 31,437 + DVU0027 0.11 -0.2 31,532 - DVU0027 0.18 -3.6 31,591 - DVU0027 0.22 -0.2 31,623 + DVU0027 0.24 -0.4 31,660 - DVU0027 0.27 -0.3 31,832 - DVU0027 0.39 -0.8 31,832 - DVU0027 0.39 -0.5 31,993 + DVU0027 0.50 +1.5 31,993 + DVU0027 0.50 -0.3 31,998 + DVU0027 0.51 -0.1 32,044 - DVU0027 0.54 -0.7
Or see this region's nucleotide sequence