Experiment: LB; anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt lytB and fkbP-2 are separated by 1 nucleotides fkbP-2 and lspA overlap by 1 nucleotides lspA and ileS are separated by 9 nucleotides ileS and ribF are separated by 32 nucleotides
SO3529: lytB - penicillin tolerance protein LytB (NCBI ptt file), at 3,683,909 to 3,684,865
lytB
SO3530: fkbP-2 - peptidyl-prolyl cis-trans isomerase FkbP (NCBI ptt file), at 3,684,867 to 3,685,286
fkbP-2
SO3531: lspA - lipoprotein signal peptidase (NCBI ptt file), at 3,685,286 to 3,685,798
lspA
SO3532: ileS - isoleucyl-tRNA synthetase (NCBI ptt file), at 3,685,808 to 3,688,630
ileS
SO3533: ribF - riboflavin biosynthesis protein RibF (NCBI ptt file), at 3,688,663 to 3,689,598
ribF
Position (kb) 3685
3686
3687
3688
3689 Strain fitness (log2 ratio) -2
-1
0
1
2 at 3684.956 kb on - strand, within fkbP-2 at 3685.083 kb on - strand, within fkbP-2 at 3685.083 kb on - strand, within fkbP-2 at 3685.117 kb on - strand, within fkbP-2 at 3685.127 kb on - strand, within fkbP-2 at 3685.208 kb on - strand, within fkbP-2
Per-strain Table
Position Strand Gene LocusTag Fraction LB; anaerobic remove 3,684,956 - fkbP-2 SO3530 0.21 -2.7 3,685,083 - fkbP-2 SO3530 0.51 -2.0 3,685,083 - fkbP-2 SO3530 0.51 -1.7 3,685,117 - fkbP-2 SO3530 0.60 +0.9 3,685,127 - fkbP-2 SO3530 0.62 +1.9 3,685,208 - fkbP-2 SO3530 0.81 -2.2
Or see this region's nucleotide sequence